Experiment: Growth at pH8 and (C) D-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0591 and PP_0592 are separated by 85 nucleotides PP_0592 and PP_0593 are separated by 368 nucleotides PP_0593 and PP_0594 are separated by 9 nucleotides
PP_0591: PP_0591 - Adenine deaminase, at 689,689 to 690,636
_0591
PP_0592: PP_0592 - Oxidoreductase, short chain dehydrogenase/reductase family, at 690,722 to 691,408
_0592
PP_0593: PP_0593 - putative ribosome-binding factor, at 691,777 to 692,361
_0593
PP_0594: PP_0594 - Transcriptional regulator, TetR family, at 692,371 to 693,114
_0594
Position (kb) 690
691
692 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 689.761 kb on - strand at 689.790 kb on - strand, within PP_0591 at 689.874 kb on + strand, within PP_0591 at 689.991 kb on + strand, within PP_0591 at 689.991 kb on + strand, within PP_0591 at 689.991 kb on + strand, within PP_0591 at 689.992 kb on - strand, within PP_0591 at 690.030 kb on - strand, within PP_0591 at 690.396 kb on - strand, within PP_0591 at 690.414 kb on - strand, within PP_0591 at 690.414 kb on - strand, within PP_0591 at 690.414 kb on - strand, within PP_0591 at 690.453 kb on - strand, within PP_0591 at 690.455 kb on + strand, within PP_0591 at 690.491 kb on + strand, within PP_0591 at 690.491 kb on + strand at 690.492 kb on - strand, within PP_0591 at 690.632 kb on + strand at 690.632 kb on + strand at 690.632 kb on + strand at 690.632 kb on + strand at 690.632 kb on + strand at 690.632 kb on + strand at 690.632 kb on + strand at 690.632 kb on + strand at 690.632 kb on + strand at 690.632 kb on + strand at 690.632 kb on + strand at 690.632 kb on + strand at 690.633 kb on - strand at 690.633 kb on - strand at 690.633 kb on - strand at 690.633 kb on - strand at 690.633 kb on - strand at 690.633 kb on - strand at 690.633 kb on - strand at 690.633 kb on - strand at 690.633 kb on - strand at 690.633 kb on - strand at 690.633 kb on - strand at 690.633 kb on - strand at 690.633 kb on - strand at 690.636 kb on + strand at 690.637 kb on - strand at 690.673 kb on + strand at 690.674 kb on - strand at 690.674 kb on - strand at 690.674 kb on - strand at 690.747 kb on + strand at 690.748 kb on - strand at 690.960 kb on + strand, within PP_0592 at 690.960 kb on + strand, within PP_0592 at 690.960 kb on + strand, within PP_0592 at 690.961 kb on - strand, within PP_0592 at 690.961 kb on - strand, within PP_0592 at 691.044 kb on - strand, within PP_0592 at 691.124 kb on + strand, within PP_0592 at 691.175 kb on + strand, within PP_0592 at 691.176 kb on - strand, within PP_0592 at 691.176 kb on - strand, within PP_0592 at 691.176 kb on - strand, within PP_0592 at 691.402 kb on + strand at 691.450 kb on + strand at 691.450 kb on + strand at 691.450 kb on + strand at 691.450 kb on + strand at 691.450 kb on + strand at 691.450 kb on + strand at 691.451 kb on - strand at 691.451 kb on - strand at 691.451 kb on - strand at 691.451 kb on - strand at 691.451 kb on - strand at 691.465 kb on - strand at 691.465 kb on - strand at 691.575 kb on + strand at 691.575 kb on + strand at 691.576 kb on - strand at 691.576 kb on - strand at 691.576 kb on - strand at 691.576 kb on - strand at 691.631 kb on + strand at 691.631 kb on + strand at 691.632 kb on - strand at 691.654 kb on + strand at 691.682 kb on + strand at 691.714 kb on + strand at 691.740 kb on + strand at 691.797 kb on + strand at 691.798 kb on - strand at 691.798 kb on - strand at 691.798 kb on - strand at 691.798 kb on - strand at 692.039 kb on + strand, within PP_0593 at 692.331 kb on - strand at 692.352 kb on + strand at 692.352 kb on + strand at 692.352 kb on + strand at 692.353 kb on - strand at 692.353 kb on - strand at 692.361 kb on + strand at 692.361 kb on + strand at 692.362 kb on - strand at 692.362 kb on - strand at 692.362 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Growth at pH8 and (C) D-Glucose remove 689,761 - -0.3 689,790 - PP_0591 0.11 +0.9 689,874 + PP_0591 0.20 +1.4 689,991 + PP_0591 0.32 -0.5 689,991 + PP_0591 0.32 +0.4 689,991 + PP_0591 0.32 -0.7 689,992 - PP_0591 0.32 +0.7 690,030 - PP_0591 0.36 +0.0 690,396 - PP_0591 0.75 -0.6 690,414 - PP_0591 0.76 +0.0 690,414 - PP_0591 0.76 +0.8 690,414 - PP_0591 0.76 -0.7 690,453 - PP_0591 0.81 -0.7 690,455 + PP_0591 0.81 +0.7 690,491 + PP_0591 0.85 +0.2 690,491 + +2.8 690,492 - PP_0591 0.85 -2.3 690,632 + -1.1 690,632 + +2.0 690,632 + +0.4 690,632 + +0.3 690,632 + -0.1 690,632 + -0.2 690,632 + -1.1 690,632 + -3.3 690,632 + +0.9 690,632 + -1.3 690,632 + -0.3 690,632 + -0.8 690,633 - +0.8 690,633 - +1.5 690,633 - +0.5 690,633 - +0.4 690,633 - -1.0 690,633 - +0.5 690,633 - +1.5 690,633 - -1.0 690,633 - -0.1 690,633 - +0.1 690,633 - +1.0 690,633 - +1.4 690,633 - +1.8 690,636 + -0.5 690,637 - -0.5 690,673 + +0.0 690,674 - -0.0 690,674 - -0.4 690,674 - +1.4 690,747 + +0.3 690,748 - -0.5 690,960 + PP_0592 0.35 -1.1 690,960 + PP_0592 0.35 -1.1 690,960 + PP_0592 0.35 -1.3 690,961 - PP_0592 0.35 -0.1 690,961 - PP_0592 0.35 -2.6 691,044 - PP_0592 0.47 -1.6 691,124 + PP_0592 0.59 -1.2 691,175 + PP_0592 0.66 -1.2 691,176 - PP_0592 0.66 -0.6 691,176 - PP_0592 0.66 -0.8 691,176 - PP_0592 0.66 -0.4 691,402 + +0.7 691,450 + -3.6 691,450 + +0.5 691,450 + +0.5 691,450 + +0.7 691,450 + +0.9 691,450 + +0.5 691,451 - +0.6 691,451 - +0.8 691,451 - -0.3 691,451 - -1.0 691,451 - +0.8 691,465 - +1.3 691,465 - +0.3 691,575 + -0.8 691,575 + +0.2 691,576 - +0.3 691,576 - +1.3 691,576 - +0.8 691,576 - +0.8 691,631 + +0.0 691,631 + +0.4 691,632 - -0.9 691,654 + +0.7 691,682 + -1.9 691,714 + +1.0 691,740 + -0.4 691,797 + -0.1 691,798 - -2.0 691,798 - -0.0 691,798 - +1.5 691,798 - -0.4 692,039 + PP_0593 0.45 -0.5 692,331 - -0.2 692,352 + +0.5 692,352 + +0.9 692,352 + -1.1 692,353 - +0.5 692,353 - +1.2 692,361 + +0.0 692,361 + -0.2 692,362 - -1.3 692,362 - +0.5 692,362 - -0.6
Or see this region's nucleotide sequence