Experiment: Growth at pH6 and (C) D-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_3047 and PP_3048 overlap by 4 nucleotides PP_3048 and PP_3049 overlap by 4 nucleotides PP_3049 and PP_3050 overlap by 8 nucleotides PP_3050 and PP_3051 overlap by 4 nucleotides PP_3051 and PP_3052 are separated by 42 nucleotides PP_3052 and PP_3053 are separated by 2 nucleotides
PP_3047: PP_3047 - conserved protein of unknown function, at 3,431,358 to 3,432,353
_3047
PP_3048: PP_3048 - conserved protein of unknown function, at 3,432,350 to 3,432,670
_3048
PP_3049: PP_3049 - conserved protein of unknown function, at 3,432,667 to 3,433,329
_3049
PP_3050: PP_3050 - conserved protein of unknown function, at 3,433,322 to 3,433,852
_3050
PP_3051: PP_3051 - Pyocin R2_PP, tail spike protein, at 3,433,849 to 3,434,451
_3051
PP_3052: PP_3052 - conserved protein of unknown function, at 3,434,494 to 3,434,763
_3052
PP_3053: PP_3053 - Pyocin R2_PP, baseplate protein, at 3,434,766 to 3,435,092
_3053
Position (kb)
3433
3434 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3432.602 kb on + strand, within PP_3048 at 3432.603 kb on - strand at 3432.603 kb on - strand, within PP_3048 at 3432.603 kb on - strand, within PP_3048 at 3432.642 kb on + strand at 3432.643 kb on - strand at 3432.643 kb on - strand at 3432.666 kb on + strand at 3432.667 kb on - strand at 3432.679 kb on - strand at 3432.679 kb on - strand at 3432.679 kb on - strand at 3432.682 kb on + strand at 3432.682 kb on + strand at 3432.852 kb on - strand, within PP_3049 at 3432.852 kb on - strand, within PP_3049 at 3432.852 kb on - strand, within PP_3049 at 3432.873 kb on + strand, within PP_3049 at 3432.873 kb on + strand, within PP_3049 at 3432.873 kb on + strand, within PP_3049 at 3433.021 kb on + strand, within PP_3049 at 3433.048 kb on + strand, within PP_3049 at 3433.077 kb on + strand, within PP_3049 at 3433.125 kb on - strand, within PP_3049 at 3433.262 kb on + strand, within PP_3049 at 3433.262 kb on + strand, within PP_3049 at 3433.262 kb on + strand, within PP_3049 at 3433.303 kb on + strand at 3433.355 kb on + strand at 3433.403 kb on + strand, within PP_3050 at 3433.404 kb on - strand, within PP_3050 at 3433.562 kb on - strand, within PP_3050 at 3433.562 kb on - strand, within PP_3050 at 3433.575 kb on + strand, within PP_3050 at 3433.575 kb on + strand, within PP_3050 at 3433.575 kb on + strand, within PP_3050 at 3433.575 kb on + strand, within PP_3050 at 3433.575 kb on + strand, within PP_3050 at 3433.575 kb on + strand, within PP_3050 at 3433.576 kb on - strand, within PP_3050 at 3433.576 kb on - strand, within PP_3050 at 3433.576 kb on - strand, within PP_3050 at 3433.576 kb on - strand, within PP_3050 at 3433.576 kb on - strand, within PP_3050 at 3433.826 kb on + strand at 3433.827 kb on - strand at 3433.827 kb on - strand at 3433.827 kb on - strand at 3433.906 kb on + strand at 3433.906 kb on + strand at 3433.907 kb on - strand at 3434.179 kb on + strand, within PP_3051 at 3434.196 kb on + strand, within PP_3051 at 3434.197 kb on - strand, within PP_3051 at 3434.200 kb on + strand, within PP_3051 at 3434.201 kb on - strand, within PP_3051 at 3434.201 kb on - strand, within PP_3051 at 3434.382 kb on - strand, within PP_3051 at 3434.494 kb on - strand at 3434.617 kb on + strand, within PP_3052 at 3434.799 kb on - strand, within PP_3053 at 3434.799 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Growth at pH6 and (C) D-Glucose remove 3,432,602 + PP_3048 0.79 +0.1 3,432,603 - +2.6 3,432,603 - PP_3048 0.79 +0.4 3,432,603 - PP_3048 0.79 +0.4 3,432,642 + +0.1 3,432,643 - +0.7 3,432,643 - +1.1 3,432,666 + -1.4 3,432,667 - +0.1 3,432,679 - -1.0 3,432,679 - +0.7 3,432,679 - +0.3 3,432,682 + +0.1 3,432,682 + -0.5 3,432,852 - PP_3049 0.28 +0.7 3,432,852 - PP_3049 0.28 -1.6 3,432,852 - PP_3049 0.28 -1.1 3,432,873 + PP_3049 0.31 +0.6 3,432,873 + PP_3049 0.31 -1.8 3,432,873 + PP_3049 0.31 +0.8 3,433,021 + PP_3049 0.53 +0.2 3,433,048 + PP_3049 0.57 -3.0 3,433,077 + PP_3049 0.62 -0.5 3,433,125 - PP_3049 0.69 -0.1 3,433,262 + PP_3049 0.90 +0.0 3,433,262 + PP_3049 0.90 +0.3 3,433,262 + PP_3049 0.90 +0.2 3,433,303 + +0.4 3,433,355 + -0.7 3,433,403 + PP_3050 0.15 +0.6 3,433,404 - PP_3050 0.15 +0.3 3,433,562 - PP_3050 0.45 -0.3 3,433,562 - PP_3050 0.45 -0.9 3,433,575 + PP_3050 0.48 +0.0 3,433,575 + PP_3050 0.48 -1.0 3,433,575 + PP_3050 0.48 +2.3 3,433,575 + PP_3050 0.48 +0.3 3,433,575 + PP_3050 0.48 +0.5 3,433,575 + PP_3050 0.48 +1.2 3,433,576 - PP_3050 0.48 +0.8 3,433,576 - PP_3050 0.48 +0.8 3,433,576 - PP_3050 0.48 +1.6 3,433,576 - PP_3050 0.48 +0.4 3,433,576 - PP_3050 0.48 +0.7 3,433,826 + +0.0 3,433,827 - +0.4 3,433,827 - -3.0 3,433,827 - +0.1 3,433,906 + -0.6 3,433,906 + -1.0 3,433,907 - +0.6 3,434,179 + PP_3051 0.55 -1.5 3,434,196 + PP_3051 0.58 +0.6 3,434,197 - PP_3051 0.58 +0.3 3,434,200 + PP_3051 0.58 -0.5 3,434,201 - PP_3051 0.58 +0.0 3,434,201 - PP_3051 0.58 -0.6 3,434,382 - PP_3051 0.88 +1.0 3,434,494 - +0.6 3,434,617 + PP_3052 0.46 +0.3 3,434,799 - PP_3053 0.10 -1.9 3,434,799 - +1.0
Or see this region's nucleotide sequence