Experiment: Growth at pH9 and (C) D-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_2028 and PP_2029 overlap by 4 nucleotides PP_2029 and PP_2030 overlap by 8 nucleotides PP_2030 and PP_2031 overlap by 4 nucleotides
PP_2028: PP_2028 - conserved protein of unknown function containing tetratricopeptide (TPR) domain repeat, at 2,304,838 to 2,306,556
_2028
PP_2029: PP_2029 - von Willebrand factor type A domain protein, at 2,306,553 to 2,307,629
_2029
PP_2030: PP_2030 - conserved protein of unknown function, at 2,307,622 to 2,308,116
_2030
PP_2031: PP_2031 - conserved protein of unknown function, at 2,308,113 to 2,309,069
_2031
Position (kb)
2306
2307
2308 Strain fitness (log2 ratio)
-1
0
1
2 at 2305.832 kb on + strand at 2305.833 kb on - strand, within PP_2028 at 2305.961 kb on - strand, within PP_2028 at 2306.141 kb on + strand, within PP_2028 at 2306.141 kb on + strand, within PP_2028 at 2306.141 kb on + strand, within PP_2028 at 2306.142 kb on - strand, within PP_2028 at 2306.413 kb on + strand at 2306.413 kb on + strand at 2306.413 kb on + strand at 2306.414 kb on - strand at 2306.414 kb on - strand at 2306.483 kb on + strand at 2306.484 kb on - strand at 2306.719 kb on + strand, within PP_2029 at 2306.828 kb on - strand, within PP_2029 at 2306.951 kb on - strand, within PP_2029 at 2306.951 kb on - strand, within PP_2029 at 2307.193 kb on + strand at 2307.193 kb on + strand, within PP_2029 at 2307.704 kb on + strand, within PP_2030 at 2307.705 kb on - strand, within PP_2030 at 2307.715 kb on + strand, within PP_2030 at 2307.715 kb on + strand, within PP_2030 at 2307.715 kb on + strand, within PP_2030 at 2307.715 kb on + strand, within PP_2030 at 2307.715 kb on + strand, within PP_2030 at 2307.716 kb on - strand, within PP_2030 at 2307.716 kb on - strand at 2307.722 kb on - strand, within PP_2030 at 2307.722 kb on - strand, within PP_2030 at 2307.887 kb on + strand, within PP_2030
Per-strain Table
Position Strand Gene LocusTag Fraction Growth at pH9 and (C) D-Glucose remove 2,305,832 + +2.1 2,305,833 - PP_2028 0.58 +0.1 2,305,961 - PP_2028 0.65 -0.3 2,306,141 + PP_2028 0.76 -1.0 2,306,141 + PP_2028 0.76 -0.1 2,306,141 + PP_2028 0.76 -0.4 2,306,142 - PP_2028 0.76 -0.8 2,306,413 + +1.4 2,306,413 + -1.5 2,306,413 + -0.6 2,306,414 - -0.5 2,306,414 - +0.1 2,306,483 + -1.6 2,306,484 - +1.2 2,306,719 + PP_2029 0.15 +0.6 2,306,828 - PP_2029 0.26 +1.7 2,306,951 - PP_2029 0.37 -0.2 2,306,951 - PP_2029 0.37 +1.0 2,307,193 + +1.6 2,307,193 + PP_2029 0.59 -0.5 2,307,704 + PP_2030 0.17 +0.6 2,307,705 - PP_2030 0.17 +1.1 2,307,715 + PP_2030 0.19 +1.3 2,307,715 + PP_2030 0.19 +0.4 2,307,715 + PP_2030 0.19 +0.4 2,307,715 + PP_2030 0.19 +1.1 2,307,715 + PP_2030 0.19 +0.6 2,307,716 - PP_2030 0.19 +0.9 2,307,716 - -0.7 2,307,722 - PP_2030 0.20 +0.3 2,307,722 - PP_2030 0.20 -1.3 2,307,887 + PP_2030 0.54 +0.2
Or see this region's nucleotide sequence