Experiment: Growth at pH9 and (C) D-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0272 and PP_0273 are separated by 65 nucleotides PP_0273 and PP_5435 are separated by 175 nucleotides PP_5435 and PP_0274 are separated by 41 nucleotides PP_0274 and PP_0275 are separated by 419 nucleotides PP_0275 and PP_0276 overlap by 4 nucleotides
PP_0272: PP_0272 - Outer membrane ferric siderophore receptor, at 330,111 to 332,408
_0272
PP_0273: PP_0273 - conserved protein of unknown function, at 332,474 to 332,686
_0273
PP_5435: PP_5435 - conserved protein of unknown function, at 332,862 to 333,215
_5435
PP_0274: PP_0274 - putative transcriptional regulator containing HTH domain, at 333,257 to 333,730
_0274
PP_0275: PP_0275 - conserved protein of unknown function, at 334,150 to 334,365
_0275
PP_0276: PP_0276 - Transcriptional regulator, Cro/CI family, at 334,362 to 334,685
_0276
Position (kb) 333
334 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 332.265 kb on + strand at 332.265 kb on + strand at 332.266 kb on - strand at 332.325 kb on + strand at 332.326 kb on - strand at 332.326 kb on - strand at 332.326 kb on - strand at 332.326 kb on - strand at 332.326 kb on - strand at 332.326 kb on - strand at 332.326 kb on - strand at 332.328 kb on + strand at 332.328 kb on + strand at 332.329 kb on - strand at 332.329 kb on - strand at 332.329 kb on - strand at 332.329 kb on - strand at 332.329 kb on - strand at 332.329 kb on - strand at 332.346 kb on + strand at 332.346 kb on + strand at 332.347 kb on - strand at 332.347 kb on - strand at 332.376 kb on - strand at 332.475 kb on + strand at 332.476 kb on - strand at 332.487 kb on - strand at 332.616 kb on + strand, within PP_0273 at 332.616 kb on + strand, within PP_0273 at 332.616 kb on + strand, within PP_0273 at 332.617 kb on - strand, within PP_0273 at 332.617 kb on - strand, within PP_0273 at 332.715 kb on + strand at 332.715 kb on + strand at 332.778 kb on + strand at 332.779 kb on - strand at 332.795 kb on + strand at 332.796 kb on - strand at 332.837 kb on + strand at 332.837 kb on + strand at 332.837 kb on + strand at 332.837 kb on + strand at 332.837 kb on + strand at 332.838 kb on - strand at 332.838 kb on - strand at 332.838 kb on - strand at 332.838 kb on - strand at 332.838 kb on - strand at 332.929 kb on + strand, within PP_5435 at 333.081 kb on - strand, within PP_5435 at 333.081 kb on - strand, within PP_5435 at 333.081 kb on - strand, within PP_5435 at 333.081 kb on - strand, within PP_5435 at 333.084 kb on + strand, within PP_5435 at 333.084 kb on + strand, within PP_5435 at 333.084 kb on + strand, within PP_5435 at 333.084 kb on + strand, within PP_5435 at 333.085 kb on - strand, within PP_5435 at 333.085 kb on - strand, within PP_5435 at 333.085 kb on - strand, within PP_5435 at 333.146 kb on + strand, within PP_5435 at 333.153 kb on + strand, within PP_5435 at 333.153 kb on + strand, within PP_5435 at 333.154 kb on - strand, within PP_5435 at 333.522 kb on + strand, within PP_0274 at 333.522 kb on + strand, within PP_0274 at 333.830 kb on - strand at 333.864 kb on - strand at 333.940 kb on + strand at 333.940 kb on + strand at 333.940 kb on + strand at 334.004 kb on + strand at 334.020 kb on + strand at 334.085 kb on - strand at 334.112 kb on + strand at 334.112 kb on + strand at 334.112 kb on + strand at 334.112 kb on + strand at 334.112 kb on + strand at 334.112 kb on + strand at 334.112 kb on + strand at 334.113 kb on - strand at 334.113 kb on - strand at 334.113 kb on - strand at 334.113 kb on - strand at 334.117 kb on + strand at 334.117 kb on + strand at 334.118 kb on - strand at 334.118 kb on - strand at 334.219 kb on + strand, within PP_0275 at 334.219 kb on + strand, within PP_0275 at 334.219 kb on + strand, within PP_0275 at 334.219 kb on + strand, within PP_0275 at 334.219 kb on + strand, within PP_0275 at 334.220 kb on - strand, within PP_0275 at 334.220 kb on - strand, within PP_0275 at 334.220 kb on - strand, within PP_0275 at 334.220 kb on - strand, within PP_0275 at 334.363 kb on + strand at 334.364 kb on - strand at 334.364 kb on - strand at 334.364 kb on - strand at 334.531 kb on - strand, within PP_0276
Per-strain Table
Position Strand Gene LocusTag Fraction Growth at pH9 and (C) D-Glucose remove 332,265 + -0.0 332,265 + +0.1 332,266 - -0.2 332,325 + -0.0 332,326 - -1.0 332,326 - +0.6 332,326 - -0.7 332,326 - +0.4 332,326 - -0.3 332,326 - +0.8 332,326 - +0.2 332,328 + -0.3 332,328 + +1.1 332,329 - +0.0 332,329 - +0.7 332,329 - +1.2 332,329 - +0.3 332,329 - +1.1 332,329 - -0.3 332,346 + -0.5 332,346 + -0.6 332,347 - -0.6 332,347 - -0.2 332,376 - +0.1 332,475 + +0.9 332,476 - +1.3 332,487 - +2.3 332,616 + PP_0273 0.67 -1.1 332,616 + PP_0273 0.67 +1.4 332,616 + PP_0273 0.67 +0.3 332,617 - PP_0273 0.67 +0.9 332,617 - PP_0273 0.67 +0.1 332,715 + +1.0 332,715 + -0.9 332,778 + -1.9 332,779 - +1.1 332,795 + -0.1 332,796 - -1.0 332,837 + +0.4 332,837 + -3.7 332,837 + +1.1 332,837 + -1.7 332,837 + -0.4 332,838 - -0.6 332,838 - -0.4 332,838 - +1.1 332,838 - +0.9 332,838 - +1.5 332,929 + PP_5435 0.19 -0.5 333,081 - PP_5435 0.62 +1.5 333,081 - PP_5435 0.62 -0.1 333,081 - PP_5435 0.62 -1.0 333,081 - PP_5435 0.62 +0.9 333,084 + PP_5435 0.63 +0.3 333,084 + PP_5435 0.63 -1.4 333,084 + PP_5435 0.63 -0.2 333,084 + PP_5435 0.63 -0.3 333,085 - PP_5435 0.63 +0.0 333,085 - PP_5435 0.63 -0.0 333,085 - PP_5435 0.63 -1.4 333,146 + PP_5435 0.80 +0.5 333,153 + PP_5435 0.82 +0.6 333,153 + PP_5435 0.82 -1.9 333,154 - PP_5435 0.82 +0.0 333,522 + PP_0274 0.56 +0.7 333,522 + PP_0274 0.56 +0.8 333,830 - +0.6 333,864 - -0.7 333,940 + -2.7 333,940 + -0.3 333,940 + -0.2 334,004 + -0.2 334,020 + +0.3 334,085 - -0.1 334,112 + -0.0 334,112 + -0.6 334,112 + -1.2 334,112 + -0.6 334,112 + +1.0 334,112 + +0.3 334,112 + +1.1 334,113 - -0.1 334,113 - +1.0 334,113 - -0.8 334,113 - +2.3 334,117 + +0.2 334,117 + +1.6 334,118 - -0.5 334,118 - +0.2 334,219 + PP_0275 0.32 -0.2 334,219 + PP_0275 0.32 -2.3 334,219 + PP_0275 0.32 -0.1 334,219 + PP_0275 0.32 +0.2 334,219 + PP_0275 0.32 -0.4 334,220 - PP_0275 0.32 -0.2 334,220 - PP_0275 0.32 +0.5 334,220 - PP_0275 0.32 +0.6 334,220 - PP_0275 0.32 +0.1 334,363 + -1.0 334,364 - -0.5 334,364 - +0.3 334,364 - +1.6 334,531 - PP_0276 0.52 +1.0
Or see this region's nucleotide sequence