Experiment: Growth at pH9 and (C) D-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0065 and PP_0066 are separated by 23 nucleotides PP_0066 and PP_0067 overlap by 4 nucleotides PP_0067 and PP_0068 are separated by 60 nucleotides
PP_0065: PP_0065 - NAD-binding component of TrK potassium transporter, at 74,836 to 76,209
_0065
PP_0066: PP_0066 - Ribosomal RNA small subunit methyltransferase B, at 76,233 to 77,543
_0066
PP_0067: PP_0067 - Methionyl-tRNA formyltransferase, at 77,540 to 78,472
_0067
PP_0068: PP_0068 - peptide deformylase, at 78,533 to 79,039
_0068
Position (kb) 76
77
78 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 75.431 kb on + strand, within PP_0065 at 75.517 kb on - strand, within PP_0065 at 75.728 kb on + strand, within PP_0065 at 75.729 kb on - strand, within PP_0065 at 75.729 kb on - strand, within PP_0065 at 75.732 kb on - strand at 75.818 kb on + strand, within PP_0065 at 75.818 kb on + strand, within PP_0065 at 75.818 kb on + strand, within PP_0065 at 75.818 kb on + strand, within PP_0065 at 75.819 kb on - strand, within PP_0065 at 76.131 kb on + strand at 76.131 kb on + strand at 76.222 kb on - strand at 76.234 kb on + strand at 76.235 kb on - strand at 76.270 kb on + strand at 76.271 kb on - strand at 76.271 kb on - strand at 76.273 kb on + strand at 76.273 kb on + strand at 76.273 kb on + strand at 76.273 kb on + strand at 76.274 kb on - strand at 76.274 kb on - strand at 76.435 kb on + strand, within PP_0066 at 76.435 kb on + strand, within PP_0066 at 76.435 kb on + strand, within PP_0066 at 76.436 kb on - strand, within PP_0066 at 76.436 kb on - strand, within PP_0066 at 76.436 kb on - strand, within PP_0066 at 76.436 kb on - strand, within PP_0066 at 76.437 kb on + strand, within PP_0066 at 76.437 kb on + strand, within PP_0066 at 76.437 kb on + strand, within PP_0066 at 76.438 kb on - strand, within PP_0066 at 76.438 kb on - strand, within PP_0066 at 76.438 kb on - strand, within PP_0066 at 76.942 kb on + strand, within PP_0066 at 76.942 kb on + strand, within PP_0066 at 76.990 kb on + strand, within PP_0066 at 76.991 kb on - strand, within PP_0066 at 76.991 kb on - strand, within PP_0066 at 76.991 kb on - strand, within PP_0066 at 77.299 kb on + strand, within PP_0066 at 77.448 kb on + strand at 77.448 kb on + strand at 77.449 kb on - strand at 77.449 kb on - strand at 77.449 kb on - strand at 77.449 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Growth at pH9 and (C) D-Glucose remove 75,431 + PP_0065 0.43 -0.2 75,517 - PP_0065 0.50 -1.3 75,728 + PP_0065 0.65 -0.6 75,729 - PP_0065 0.65 -0.8 75,729 - PP_0065 0.65 -0.4 75,732 - +1.4 75,818 + PP_0065 0.71 +0.7 75,818 + PP_0065 0.71 -1.3 75,818 + PP_0065 0.71 -1.3 75,818 + PP_0065 0.71 +0.9 75,819 - PP_0065 0.72 -1.3 76,131 + -1.8 76,131 + +0.9 76,222 - -1.6 76,234 + -1.9 76,235 - -0.0 76,270 + +0.9 76,271 - -0.9 76,271 - +0.7 76,273 + -3.7 76,273 + +0.9 76,273 + -0.6 76,273 + +0.2 76,274 - +0.2 76,274 - -0.8 76,435 + PP_0066 0.15 +0.1 76,435 + PP_0066 0.15 +2.6 76,435 + PP_0066 0.15 +0.8 76,436 - PP_0066 0.15 +0.6 76,436 - PP_0066 0.15 +0.9 76,436 - PP_0066 0.15 +0.4 76,436 - PP_0066 0.15 +0.4 76,437 + PP_0066 0.16 +1.3 76,437 + PP_0066 0.16 +0.1 76,437 + PP_0066 0.16 +0.6 76,438 - PP_0066 0.16 -0.2 76,438 - PP_0066 0.16 +1.1 76,438 - PP_0066 0.16 -0.5 76,942 + PP_0066 0.54 -0.4 76,942 + PP_0066 0.54 +1.5 76,990 + PP_0066 0.58 +1.8 76,991 - PP_0066 0.58 +0.6 76,991 - PP_0066 0.58 +0.8 76,991 - PP_0066 0.58 +1.0 77,299 + PP_0066 0.81 +1.4 77,448 + +0.5 77,448 + +0.7 77,449 - +0.8 77,449 - -0.2 77,449 - +0.0 77,449 - +0.3
Or see this region's nucleotide sequence