Experiment: Growth at pH9 and (C) D-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_3417 and PP_3418 are separated by 86 nucleotides PP_3418 and PP_3419 are separated by 14 nucleotides PP_3419 and PP_3420 overlap by 32 nucleotides
PP_3417: PP_3417 - D-gluconate transporter, at 3,867,735 to 3,869,087
_3417
PP_3418: PP_3418 - conserved protein of unknown function, at 3,869,174 to 3,869,476
_3418
PP_3419: PP_3419 - Sigma-54 dependent transcriptional regulator/response regulator, at 3,869,491 to 3,870,918
_3419
PP_3420: PP_3420 - Sensor histidine kinase, at 3,870,887 to 3,872,380
_3420
Position (kb)
3869
3870
3871 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3868.856 kb on + strand, within PP_3417 at 3868.909 kb on + strand, within PP_3417 at 3868.981 kb on - strand at 3868.981 kb on - strand at 3868.981 kb on - strand at 3869.101 kb on + strand at 3869.140 kb on + strand at 3869.140 kb on + strand at 3869.140 kb on + strand at 3869.141 kb on - strand at 3869.141 kb on - strand at 3869.141 kb on - strand at 3869.141 kb on - strand at 3869.156 kb on - strand at 3869.156 kb on - strand at 3869.347 kb on - strand, within PP_3418 at 3869.467 kb on + strand at 3869.490 kb on - strand at 3869.490 kb on - strand at 3869.492 kb on + strand at 3869.832 kb on + strand, within PP_3419 at 3869.832 kb on + strand, within PP_3419 at 3869.832 kb on + strand, within PP_3419 at 3869.832 kb on + strand, within PP_3419 at 3869.833 kb on - strand, within PP_3419 at 3869.833 kb on - strand, within PP_3419 at 3870.184 kb on - strand, within PP_3419 at 3870.601 kb on + strand, within PP_3419 at 3870.839 kb on + strand at 3870.840 kb on - strand at 3870.857 kb on + strand at 3870.858 kb on - strand at 3870.938 kb on - strand at 3870.946 kb on + strand at 3870.946 kb on + strand at 3870.947 kb on - strand at 3870.947 kb on - strand at 3870.947 kb on - strand at 3870.947 kb on - strand at 3871.508 kb on + strand, within PP_3420 at 3871.508 kb on + strand, within PP_3420 at 3871.509 kb on - strand, within PP_3420 at 3871.509 kb on - strand, within PP_3420 at 3871.509 kb on - strand, within PP_3420 at 3871.572 kb on + strand, within PP_3420 at 3871.573 kb on - strand, within PP_3420 at 3871.615 kb on + strand, within PP_3420 at 3871.615 kb on + strand, within PP_3420 at 3871.615 kb on + strand, within PP_3420 at 3871.615 kb on + strand, within PP_3420 at 3871.615 kb on + strand, within PP_3420 at 3871.615 kb on + strand, within PP_3420 at 3871.615 kb on + strand, within PP_3420 at 3871.615 kb on + strand, within PP_3420 at 3871.615 kb on + strand, within PP_3420 at 3871.616 kb on - strand, within PP_3420 at 3871.616 kb on - strand, within PP_3420 at 3871.616 kb on - strand, within PP_3420 at 3871.616 kb on - strand, within PP_3420 at 3871.616 kb on - strand, within PP_3420 at 3871.653 kb on + strand, within PP_3420 at 3871.653 kb on + strand, within PP_3420 at 3871.654 kb on - strand, within PP_3420 at 3871.654 kb on - strand, within PP_3420 at 3871.698 kb on + strand, within PP_3420 at 3871.699 kb on - strand, within PP_3420 at 3871.761 kb on - strand, within PP_3420 at 3871.761 kb on - strand, within PP_3420 at 3871.794 kb on + strand, within PP_3420 at 3871.812 kb on + strand, within PP_3420 at 3871.813 kb on - strand, within PP_3420 at 3871.813 kb on - strand, within PP_3420 at 3871.867 kb on - strand, within PP_3420 at 3871.867 kb on - strand, within PP_3420
Per-strain Table
Position Strand Gene LocusTag Fraction Growth at pH9 and (C) D-Glucose remove 3,868,856 + PP_3417 0.83 -3.1 3,868,909 + PP_3417 0.87 -3.7 3,868,981 - -3.1 3,868,981 - -2.0 3,868,981 - -1.7 3,869,101 + -1.0 3,869,140 + -1.8 3,869,140 + -0.4 3,869,140 + -0.9 3,869,141 - -1.3 3,869,141 - -1.9 3,869,141 - -3.4 3,869,141 - +0.7 3,869,156 - -0.5 3,869,156 - -0.1 3,869,347 - PP_3418 0.57 +0.1 3,869,467 + +0.7 3,869,490 - -1.6 3,869,490 - -0.9 3,869,492 + -0.3 3,869,832 + PP_3419 0.24 +0.2 3,869,832 + PP_3419 0.24 -0.1 3,869,832 + PP_3419 0.24 -0.2 3,869,832 + PP_3419 0.24 +0.2 3,869,833 - PP_3419 0.24 -0.7 3,869,833 - PP_3419 0.24 -0.3 3,870,184 - PP_3419 0.49 -1.2 3,870,601 + PP_3419 0.78 -0.4 3,870,839 + +0.7 3,870,840 - -0.2 3,870,857 + +0.6 3,870,858 - +0.1 3,870,938 - -1.6 3,870,946 + +2.1 3,870,946 + +0.3 3,870,947 - -1.1 3,870,947 - -3.8 3,870,947 - -2.6 3,870,947 - -0.0 3,871,508 + PP_3420 0.42 -0.3 3,871,508 + PP_3420 0.42 -0.2 3,871,509 - PP_3420 0.42 +1.1 3,871,509 - PP_3420 0.42 -3.0 3,871,509 - PP_3420 0.42 +0.1 3,871,572 + PP_3420 0.46 +0.8 3,871,573 - PP_3420 0.46 +0.3 3,871,615 + PP_3420 0.49 +0.7 3,871,615 + PP_3420 0.49 +0.3 3,871,615 + PP_3420 0.49 -0.9 3,871,615 + PP_3420 0.49 +0.1 3,871,615 + PP_3420 0.49 +0.4 3,871,615 + PP_3420 0.49 +0.7 3,871,615 + PP_3420 0.49 +0.2 3,871,615 + PP_3420 0.49 -0.0 3,871,615 + PP_3420 0.49 -3.1 3,871,616 - PP_3420 0.49 +0.3 3,871,616 - PP_3420 0.49 -0.2 3,871,616 - PP_3420 0.49 -1.8 3,871,616 - PP_3420 0.49 +0.2 3,871,616 - PP_3420 0.49 +0.4 3,871,653 + PP_3420 0.51 +0.7 3,871,653 + PP_3420 0.51 +0.4 3,871,654 - PP_3420 0.51 -0.8 3,871,654 - PP_3420 0.51 -2.8 3,871,698 + PP_3420 0.54 +0.9 3,871,699 - PP_3420 0.54 +0.9 3,871,761 - PP_3420 0.59 +0.2 3,871,761 - PP_3420 0.59 +0.8 3,871,794 + PP_3420 0.61 +0.6 3,871,812 + PP_3420 0.62 -0.1 3,871,813 - PP_3420 0.62 +0.1 3,871,813 - PP_3420 0.62 +0.3 3,871,867 - PP_3420 0.66 +0.5 3,871,867 - PP_3420 0.66 -2.4
Or see this region's nucleotide sequence