Experiment: Bas67
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt traI and vapB are separated by 81 nucleotides vapB and vapC overlap by 1 nucleotides vapC and traD are separated by 8 nucleotides
NOLOHH_24685: traI - conjugative transfer relaxase/helicase TraI, at 75,927 to 81,197
traI
NOLOHH_24695: vapB - toxin-antitoxin system antitoxin VapB, at 81,279 to 81,506
vapB
NOLOHH_24700: vapC - tRNA(fMet)-specific endonuclease VapC, at 81,506 to 81,904
vapC
NOLOHH_24705: traD - type IV conjugative transfer system coupling protein TraD, at 81,913 to 84,102
traD
Position (kb) 81
82 Strain fitness (log2 ratio) -2
-1
0
1
2 at 80.630 kb on - strand, within traI at 80.630 kb on - strand, within traI at 80.730 kb on + strand at 80.730 kb on + strand at 80.731 kb on - strand at 80.731 kb on - strand at 80.766 kb on - strand at 80.879 kb on + strand at 80.880 kb on - strand at 80.891 kb on + strand at 81.042 kb on - strand at 81.127 kb on + strand at 81.127 kb on + strand at 81.128 kb on - strand at 81.128 kb on - strand at 81.128 kb on - strand at 81.128 kb on - strand at 81.130 kb on + strand at 81.130 kb on + strand at 81.130 kb on + strand at 81.131 kb on - strand at 81.131 kb on - strand at 81.131 kb on - strand at 81.134 kb on + strand at 81.149 kb on - strand at 81.151 kb on + strand at 81.151 kb on + strand at 81.151 kb on + strand at 81.151 kb on + strand at 81.151 kb on + strand at 81.152 kb on - strand at 81.152 kb on - strand at 81.188 kb on + strand at 81.246 kb on + strand at 81.246 kb on + strand at 81.247 kb on - strand at 81.247 kb on - strand at 81.247 kb on - strand at 81.258 kb on - strand at 81.258 kb on - strand at 81.265 kb on + strand at 81.293 kb on - strand at 81.293 kb on - strand at 81.355 kb on + strand, within vapB at 81.459 kb on - strand, within vapB at 81.459 kb on - strand, within vapB at 81.459 kb on - strand, within vapB at 81.544 kb on + strand at 81.636 kb on + strand, within vapC at 81.636 kb on + strand, within vapC at 81.636 kb on + strand, within vapC at 81.636 kb on + strand, within vapC at 81.636 kb on + strand, within vapC at 81.637 kb on - strand, within vapC at 81.638 kb on + strand, within vapC at 81.638 kb on + strand, within vapC at 81.638 kb on + strand, within vapC at 81.638 kb on + strand, within vapC at 81.638 kb on + strand, within vapC at 81.638 kb on + strand, within vapC at 81.639 kb on - strand, within vapC at 81.639 kb on - strand, within vapC at 81.639 kb on - strand, within vapC at 81.639 kb on - strand, within vapC at 81.639 kb on - strand, within vapC at 81.848 kb on - strand, within vapC at 82.340 kb on - strand, within traD at 82.416 kb on + strand, within traD at 82.466 kb on + strand, within traD at 82.466 kb on + strand, within traD at 82.466 kb on + strand, within traD at 82.466 kb on + strand, within traD at 82.467 kb on - strand, within traD at 82.467 kb on - strand, within traD at 82.467 kb on - strand, within traD at 82.502 kb on + strand, within traD at 82.503 kb on - strand, within traD at 82.503 kb on - strand, within traD at 82.556 kb on - strand, within traD at 82.642 kb on - strand, within traD at 82.712 kb on + strand, within traD at 82.712 kb on + strand, within traD at 82.712 kb on + strand, within traD at 82.713 kb on - strand, within traD at 82.713 kb on - strand, within traD at 82.713 kb on - strand, within traD at 82.717 kb on - strand, within traD at 82.717 kb on - strand, within traD at 82.754 kb on + strand, within traD at 82.755 kb on - strand, within traD
Per-strain Table
Position Strand Gene LocusTag Fraction Bas67 remove 80,630 - traI NOLOHH_24685 0.89 -0.1 80,630 - traI NOLOHH_24685 0.89 -0.4 80,730 + +0.9 80,730 + -0.7 80,731 - +1.8 80,731 - -2.0 80,766 - +0.9 80,879 + +1.1 80,880 - +1.9 80,891 + +0.9 81,042 - -0.5 81,127 + +0.3 81,127 + +2.2 81,128 - +0.8 81,128 - -0.7 81,128 - +0.9 81,128 - +0.7 81,130 + -0.7 81,130 + +0.7 81,130 + +0.9 81,131 - +0.9 81,131 - +0.6 81,131 - -0.4 81,134 + +0.2 81,149 - -0.1 81,151 + +1.4 81,151 + +0.4 81,151 + +0.4 81,151 + -0.1 81,151 + +0.3 81,152 - -1.8 81,152 - -0.5 81,188 + -0.7 81,246 + +0.6 81,246 + -0.5 81,247 - -1.5 81,247 - -0.5 81,247 - +0.7 81,258 - +1.4 81,258 - -0.7 81,265 + +1.7 81,293 - +0.1 81,293 - -2.0 81,355 + vapB NOLOHH_24695 0.33 -0.5 81,459 - vapB NOLOHH_24695 0.79 +0.9 81,459 - vapB NOLOHH_24695 0.79 +0.3 81,459 - vapB NOLOHH_24695 0.79 -0.9 81,544 + +1.3 81,636 + vapC NOLOHH_24700 0.33 +0.4 81,636 + vapC NOLOHH_24700 0.33 -0.9 81,636 + vapC NOLOHH_24700 0.33 -1.3 81,636 + vapC NOLOHH_24700 0.33 -1.3 81,636 + vapC NOLOHH_24700 0.33 +0.2 81,637 - vapC NOLOHH_24700 0.33 -0.9 81,638 + vapC NOLOHH_24700 0.33 +0.4 81,638 + vapC NOLOHH_24700 0.33 -0.4 81,638 + vapC NOLOHH_24700 0.33 -0.1 81,638 + vapC NOLOHH_24700 0.33 +1.6 81,638 + vapC NOLOHH_24700 0.33 +0.9 81,638 + vapC NOLOHH_24700 0.33 +0.3 81,639 - vapC NOLOHH_24700 0.33 -0.3 81,639 - vapC NOLOHH_24700 0.33 -1.6 81,639 - vapC NOLOHH_24700 0.33 -1.9 81,639 - vapC NOLOHH_24700 0.33 -1.1 81,639 - vapC NOLOHH_24700 0.33 -1.0 81,848 - vapC NOLOHH_24700 0.86 +0.4 82,340 - traD NOLOHH_24705 0.19 +0.2 82,416 + traD NOLOHH_24705 0.23 -0.1 82,466 + traD NOLOHH_24705 0.25 -0.4 82,466 + traD NOLOHH_24705 0.25 -0.7 82,466 + traD NOLOHH_24705 0.25 +0.9 82,466 + traD NOLOHH_24705 0.25 +2.4 82,467 - traD NOLOHH_24705 0.25 +0.3 82,467 - traD NOLOHH_24705 0.25 -0.0 82,467 - traD NOLOHH_24705 0.25 +0.9 82,502 + traD NOLOHH_24705 0.27 +0.3 82,503 - traD NOLOHH_24705 0.27 +0.9 82,503 - traD NOLOHH_24705 0.27 -0.6 82,556 - traD NOLOHH_24705 0.29 -0.5 82,642 - traD NOLOHH_24705 0.33 -0.6 82,712 + traD NOLOHH_24705 0.36 -1.5 82,712 + traD NOLOHH_24705 0.36 +1.9 82,712 + traD NOLOHH_24705 0.36 -0.1 82,713 - traD NOLOHH_24705 0.37 -0.8 82,713 - traD NOLOHH_24705 0.37 -0.1 82,713 - traD NOLOHH_24705 0.37 -0.4 82,717 - traD NOLOHH_24705 0.37 -0.4 82,717 - traD NOLOHH_24705 0.37 +0.1 82,754 + traD NOLOHH_24705 0.38 +0.9 82,755 - traD NOLOHH_24705 0.38 +0.9
Or see this region's nucleotide sequence