Strain Fitness in Escherichia coli ECOR27 around NOLOHH_24280

Experiment: Bas67

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntpsiA and psiB overlap by 4 nucleotidespsiB and yubM are separated by 54 nucleotidesyubM and yubL are separated by 65 nucleotidesyubL and ssb are separated by 57 nucleotides NOLOHH_24270: psiA - Protein PsiA, at 6,275 to 6,994 psiA NOLOHH_24275: psiB - conjugation system SOS inhibitor PsiB, at 6,991 to 7,425 psiB NOLOHH_24280: yubM - Uncharacterized protein YubM, at 7,480 to 9,444 yubM NOLOHH_24285: yubL - UPF0401 protein YubL, at 9,510 to 9,743 yubL NOLOHH_24290: ssb - single-stranded DNA-binding protein, at 9,801 to 10,328 ssb Position (kb) 7 8 9 10Strain fitness (log2 ratio) -2 -1 0 1 2 3at 6.613 kb on - strand, within psiAat 6.756 kb on + strand, within psiAat 6.756 kb on + strand, within psiAat 6.757 kb on - strand, within psiAat 6.876 kb on + strand, within psiAat 7.020 kb on + strandat 7.195 kb on + strand, within psiBat 7.196 kb on - strand, within psiBat 7.196 kb on - strand, within psiBat 7.373 kb on + strand, within psiBat 7.396 kb on + strandat 7.396 kb on + strandat 7.397 kb on - strandat 7.397 kb on - strandat 7.399 kb on + strandat 7.399 kb on + strandat 7.399 kb on + strandat 7.508 kb on + strandat 7.540 kb on + strandat 7.540 kb on + strandat 7.540 kb on + strandat 7.540 kb on + strandat 7.541 kb on - strandat 7.581 kb on + strandat 7.920 kb on + strand, within yubMat 7.920 kb on + strand, within yubMat 7.920 kb on + strand, within yubMat 8.004 kb on + strand, within yubMat 8.005 kb on - strand, within yubMat 8.005 kb on - strand, within yubMat 8.032 kb on - strand, within yubMat 8.484 kb on - strand, within yubMat 8.484 kb on - strand, within yubMat 8.489 kb on + strand, within yubMat 8.489 kb on + strand, within yubMat 8.489 kb on + strand, within yubMat 8.490 kb on - strand, within yubMat 8.490 kb on - strand, within yubMat 8.490 kb on - strand, within yubMat 8.490 kb on - strand, within yubMat 8.490 kb on - strand, within yubMat 8.530 kb on - strand, within yubMat 8.630 kb on + strand, within yubMat 8.631 kb on - strand, within yubMat 8.631 kb on - strand, within yubMat 8.710 kb on - strand, within yubMat 8.815 kb on + strand, within yubMat 8.815 kb on + strand, within yubMat 9.107 kb on - strand, within yubMat 9.244 kb on - strand, within yubMat 9.312 kb on + strandat 9.396 kb on + strandat 9.426 kb on + strandat 9.435 kb on + strandat 9.444 kb on + strandat 9.444 kb on + strandat 9.444 kb on + strandat 9.444 kb on + strandat 9.444 kb on + strandat 9.444 kb on + strandat 9.445 kb on - strandat 9.448 kb on + strandat 9.448 kb on + strandat 9.448 kb on + strandat 9.448 kb on + strandat 9.448 kb on + strandat 9.448 kb on + strandat 9.448 kb on + strandat 9.449 kb on - strandat 9.449 kb on - strandat 9.449 kb on - strandat 9.449 kb on - strandat 9.452 kb on - strandat 9.452 kb on - strandat 9.469 kb on + strandat 9.469 kb on + strandat 9.515 kb on + strandat 9.723 kb on + strandat 9.878 kb on + strand, within ssbat 9.879 kb on - strand, within ssbat 10.040 kb on - strand, within ssbat 10.050 kb on - strand, within ssbat 10.093 kb on + strand, within ssbat 10.118 kb on - strand, within ssbat 10.118 kb on - strand, within ssbat 10.262 kb on - strand, within ssbat 10.262 kb on - strand, within ssbat 10.334 kb on - strandat 10.334 kb on - strandat 10.334 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas67
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6,613 - psiA NOLOHH_24270 0.47 +0.3
6,756 + psiA NOLOHH_24270 0.67 -0.6
6,756 + psiA NOLOHH_24270 0.67 +0.4
6,757 - psiA NOLOHH_24270 0.67 +1.9
6,876 + psiA NOLOHH_24270 0.83 -1.9
7,020 + -0.1
7,195 + psiB NOLOHH_24275 0.47 +0.1
7,196 - psiB NOLOHH_24275 0.47 +0.6
7,196 - psiB NOLOHH_24275 0.47 +1.6
7,373 + psiB NOLOHH_24275 0.88 +1.3
7,396 + +0.6
7,396 + +1.9
7,397 - +0.4
7,397 - -0.1
7,399 + -1.0
7,399 + +0.3
7,399 + +0.2
7,508 + +1.4
7,540 + +1.9
7,540 + -0.1
7,540 + -1.1
7,540 + -1.5
7,541 - +1.1
7,581 + +1.2
7,920 + yubM NOLOHH_24280 0.22 +1.9
7,920 + yubM NOLOHH_24280 0.22 +0.9
7,920 + yubM NOLOHH_24280 0.22 -0.7
8,004 + yubM NOLOHH_24280 0.27 +0.3
8,005 - yubM NOLOHH_24280 0.27 -1.1
8,005 - yubM NOLOHH_24280 0.27 -2.1
8,032 - yubM NOLOHH_24280 0.28 +0.7
8,484 - yubM NOLOHH_24280 0.51 -0.1
8,484 - yubM NOLOHH_24280 0.51 +0.1
8,489 + yubM NOLOHH_24280 0.51 +0.3
8,489 + yubM NOLOHH_24280 0.51 +0.9
8,489 + yubM NOLOHH_24280 0.51 -0.7
8,490 - yubM NOLOHH_24280 0.51 -1.1
8,490 - yubM NOLOHH_24280 0.51 +0.4
8,490 - yubM NOLOHH_24280 0.51 +1.9
8,490 - yubM NOLOHH_24280 0.51 +0.3
8,490 - yubM NOLOHH_24280 0.51 +1.6
8,530 - yubM NOLOHH_24280 0.53 +1.6
8,630 + yubM NOLOHH_24280 0.59 -0.9
8,631 - yubM NOLOHH_24280 0.59 +1.4
8,631 - yubM NOLOHH_24280 0.59 -0.7
8,710 - yubM NOLOHH_24280 0.63 -0.4
8,815 + yubM NOLOHH_24280 0.68 +3.2
8,815 + yubM NOLOHH_24280 0.68 +0.9
9,107 - yubM NOLOHH_24280 0.83 +0.9
9,244 - yubM NOLOHH_24280 0.90 +1.4
9,312 + +0.4
9,396 + -0.1
9,426 + +1.1
9,435 + +0.9
9,444 + +0.4
9,444 + -0.7
9,444 + -0.4
9,444 + +2.1
9,444 + +0.2
9,444 + -1.7
9,445 - +0.3
9,448 + +1.3
9,448 + +0.2
9,448 + +0.4
9,448 + +0.3
9,448 + +1.1
9,448 + +0.3
9,448 + -0.3
9,449 - -0.4
9,449 - +0.4
9,449 - -0.9
9,449 - +0.5
9,452 - +0.3
9,452 - -0.7
9,469 + -0.7
9,469 + +1.4
9,515 + -1.1
9,723 + +1.9
9,878 + ssb NOLOHH_24290 0.15 -0.1
9,879 - ssb NOLOHH_24290 0.15 +0.9
10,040 - ssb NOLOHH_24290 0.45 -0.7
10,050 - ssb NOLOHH_24290 0.47 +1.4
10,093 + ssb NOLOHH_24290 0.55 -0.1
10,118 - ssb NOLOHH_24290 0.60 -1.9
10,118 - ssb NOLOHH_24290 0.60 -0.7
10,262 - ssb NOLOHH_24290 0.87 +0.9
10,262 - ssb NOLOHH_24290 0.87 -1.5
10,334 - +0.9
10,334 - +0.9
10,334 - +0.9

Or see this region's nucleotide sequence