Strain Fitness in Escherichia coli ECOR27 around NOLOHH_23750

Experiment: Bas67

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntmrdB and rlpA are separated by 10 nucleotidesrlpA and dacA are separated by 66 nucleotides NOLOHH_23745: mrdB - peptidoglycan glycosyltransferase MrdB, at 4,673,608 to 4,674,720 mrdB NOLOHH_23750: rlpA - endolytic peptidoglycan transglycosylase RlpA, at 4,674,731 to 4,675,819 rlpA NOLOHH_23755: dacA - D-alanyl-D-alanine carboxypeptidase DacA, at 4,675,886 to 4,677,169 dacA Position (kb) 4674 4675 4676Strain fitness (log2 ratio) -1 0 1 2 3at 4674.784 kb on + strandat 4674.834 kb on + strandat 4674.834 kb on + strandat 4674.986 kb on + strand, within rlpAat 4674.986 kb on + strand, within rlpAat 4674.987 kb on - strand, within rlpAat 4675.165 kb on + strand, within rlpAat 4675.199 kb on - strand, within rlpAat 4675.274 kb on - strand, within rlpAat 4675.282 kb on + strand, within rlpAat 4675.304 kb on + strand, within rlpAat 4675.467 kb on + strand, within rlpAat 4675.603 kb on + strand, within rlpAat 4675.604 kb on - strand, within rlpAat 4675.604 kb on - strand, within rlpAat 4675.604 kb on - strand, within rlpAat 4675.791 kb on + strandat 4675.801 kb on + strandat 4675.801 kb on + strandat 4675.801 kb on + strandat 4675.801 kb on + strandat 4675.802 kb on - strandat 4675.802 kb on - strandat 4675.818 kb on - strandat 4675.822 kb on + strandat 4675.911 kb on + strandat 4675.911 kb on + strandat 4675.911 kb on + strandat 4675.911 kb on + strandat 4675.912 kb on - strandat 4675.947 kb on + strandat 4675.947 kb on + strandat 4675.982 kb on - strandat 4676.027 kb on - strand, within dacAat 4676.027 kb on - strand, within dacAat 4676.065 kb on + strand, within dacAat 4676.102 kb on + strand, within dacAat 4676.118 kb on - strand, within dacAat 4676.118 kb on - strand, within dacAat 4676.119 kb on + strand, within dacAat 4676.198 kb on + strand, within dacAat 4676.199 kb on - strand, within dacAat 4676.199 kb on - strand, within dacAat 4676.362 kb on + strand, within dacAat 4676.450 kb on + strand, within dacAat 4676.450 kb on + strand, within dacAat 4676.450 kb on + strand, within dacAat 4676.451 kb on - strand, within dacAat 4676.451 kb on - strand, within dacAat 4676.493 kb on + strand, within dacAat 4676.536 kb on + strand, within dacAat 4676.591 kb on + strand, within dacAat 4676.593 kb on + strand, within dacAat 4676.621 kb on - strand, within dacA

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas67
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4,674,784 + +1.4
4,674,834 + -0.2
4,674,834 + +1.4
4,674,986 + rlpA NOLOHH_23750 0.23 +0.4
4,674,986 + rlpA NOLOHH_23750 0.23 +0.9
4,674,987 - rlpA NOLOHH_23750 0.24 +0.4
4,675,165 + rlpA NOLOHH_23750 0.40 -0.6
4,675,199 - rlpA NOLOHH_23750 0.43 -1.6
4,675,274 - rlpA NOLOHH_23750 0.50 +1.4
4,675,282 + rlpA NOLOHH_23750 0.51 +1.0
4,675,304 + rlpA NOLOHH_23750 0.53 -0.6
4,675,467 + rlpA NOLOHH_23750 0.68 -1.0
4,675,603 + rlpA NOLOHH_23750 0.80 -0.5
4,675,604 - rlpA NOLOHH_23750 0.80 -1.6
4,675,604 - rlpA NOLOHH_23750 0.80 +1.1
4,675,604 - rlpA NOLOHH_23750 0.80 -0.6
4,675,791 + -0.3
4,675,801 + +1.8
4,675,801 + +1.2
4,675,801 + -0.4
4,675,801 + -0.0
4,675,802 - -1.7
4,675,802 - +1.8
4,675,818 - +1.4
4,675,822 + -0.4
4,675,911 + +1.0
4,675,911 + -0.2
4,675,911 + -0.1
4,675,911 + +0.9
4,675,912 - -0.3
4,675,947 + +0.1
4,675,947 + +0.4
4,675,982 - -0.1
4,676,027 - dacA NOLOHH_23755 0.11 +0.4
4,676,027 - dacA NOLOHH_23755 0.11 +1.4
4,676,065 + dacA NOLOHH_23755 0.14 +1.8
4,676,102 + dacA NOLOHH_23755 0.17 -0.9
4,676,118 - dacA NOLOHH_23755 0.18 +0.4
4,676,118 - dacA NOLOHH_23755 0.18 +2.2
4,676,119 + dacA NOLOHH_23755 0.18 +3.4
4,676,198 + dacA NOLOHH_23755 0.24 +0.0
4,676,199 - dacA NOLOHH_23755 0.24 +0.9
4,676,199 - dacA NOLOHH_23755 0.24 +1.2
4,676,362 + dacA NOLOHH_23755 0.37 +2.0
4,676,450 + dacA NOLOHH_23755 0.44 -0.6
4,676,450 + dacA NOLOHH_23755 0.44 -1.1
4,676,450 + dacA NOLOHH_23755 0.44 -1.6
4,676,451 - dacA NOLOHH_23755 0.44 +1.0
4,676,451 - dacA NOLOHH_23755 0.44 -0.1
4,676,493 + dacA NOLOHH_23755 0.47 +1.4
4,676,536 + dacA NOLOHH_23755 0.51 +1.4
4,676,591 + dacA NOLOHH_23755 0.55 +0.6
4,676,593 + dacA NOLOHH_23755 0.55 +1.0
4,676,621 - dacA NOLOHH_23755 0.57 -1.1

Or see this region's nucleotide sequence