Strain Fitness in Escherichia coli ECOR27 around NOLOHH_23745

Experiment: Bas67

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntmrdA and mrdB are separated by 2 nucleotidesmrdB and rlpA are separated by 10 nucleotides NOLOHH_23740: mrdA - peptidoglycan DD-transpeptidase MrdA, at 4,671,704 to 4,673,605 mrdA NOLOHH_23745: mrdB - peptidoglycan glycosyltransferase MrdB, at 4,673,608 to 4,674,720 mrdB NOLOHH_23750: rlpA - endolytic peptidoglycan transglycosylase RlpA, at 4,674,731 to 4,675,819 rlpA Position (kb) 4673 4674 4675Strain fitness (log2 ratio) -1 0 1at 4674.784 kb on + strandat 4674.834 kb on + strandat 4674.834 kb on + strandat 4674.986 kb on + strand, within rlpAat 4674.986 kb on + strand, within rlpAat 4674.987 kb on - strand, within rlpAat 4675.165 kb on + strand, within rlpAat 4675.199 kb on - strand, within rlpAat 4675.274 kb on - strand, within rlpAat 4675.282 kb on + strand, within rlpAat 4675.304 kb on + strand, within rlpAat 4675.467 kb on + strand, within rlpAat 4675.603 kb on + strand, within rlpAat 4675.604 kb on - strand, within rlpAat 4675.604 kb on - strand, within rlpAat 4675.604 kb on - strand, within rlpA

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas67
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4,674,784 + +1.4
4,674,834 + -0.2
4,674,834 + +1.4
4,674,986 + rlpA NOLOHH_23750 0.23 +0.4
4,674,986 + rlpA NOLOHH_23750 0.23 +0.9
4,674,987 - rlpA NOLOHH_23750 0.24 +0.4
4,675,165 + rlpA NOLOHH_23750 0.40 -0.6
4,675,199 - rlpA NOLOHH_23750 0.43 -1.6
4,675,274 - rlpA NOLOHH_23750 0.50 +1.4
4,675,282 + rlpA NOLOHH_23750 0.51 +1.0
4,675,304 + rlpA NOLOHH_23750 0.53 -0.6
4,675,467 + rlpA NOLOHH_23750 0.68 -1.0
4,675,603 + rlpA NOLOHH_23750 0.80 -0.5
4,675,604 - rlpA NOLOHH_23750 0.80 -1.6
4,675,604 - rlpA NOLOHH_23750 0.80 +1.1
4,675,604 - rlpA NOLOHH_23750 0.80 -0.6

Or see this region's nucleotide sequence