Strain Fitness in Escherichia coli ECOR27 around NOLOHH_23740

Experiment: Bas67

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntrsfS and rlmH are separated by 3 nucleotidesrlmH and mrdA are separated by 30 nucleotidesmrdA and mrdB are separated by 2 nucleotides NOLOHH_23730: rsfS - ribosome silencing factor, at 4,670,885 to 4,671,202 rsfS NOLOHH_23735: rlmH - 23S rRNA (pseudouridine(1915)-N(3))-methyltransferase RlmH, at 4,671,206 to 4,671,673 rlmH NOLOHH_23740: mrdA - peptidoglycan DD-transpeptidase MrdA, at 4,671,704 to 4,673,605 mrdA NOLOHH_23745: mrdB - peptidoglycan glycosyltransferase MrdB, at 4,673,608 to 4,674,720 mrdB Position (kb) 4671 4672 4673 4674Strain fitness (log2 ratio) -1 0 1at 4670.725 kb on + strandat 4670.837 kb on + strandat 4670.837 kb on + strandat 4670.860 kb on + strandat 4670.958 kb on + strand, within rsfSat 4671.004 kb on + strand, within rsfSat 4671.028 kb on + strand, within rsfSat 4671.028 kb on + strand, within rsfSat 4671.028 kb on + strand, within rsfSat 4671.028 kb on + strand, within rsfSat 4671.028 kb on + strand, within rsfSat 4671.272 kb on + strand, within rlmHat 4671.504 kb on + strand, within rlmHat 4671.541 kb on + strand, within rlmHat 4671.674 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas67
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4,670,725 + +0.3
4,670,837 + +0.3
4,670,837 + +0.2
4,670,860 + +0.8
4,670,958 + rsfS NOLOHH_23730 0.23 +0.4
4,671,004 + rsfS NOLOHH_23730 0.37 +1.3
4,671,028 + rsfS NOLOHH_23730 0.45 +1.4
4,671,028 + rsfS NOLOHH_23730 0.45 +1.4
4,671,028 + rsfS NOLOHH_23730 0.45 -0.1
4,671,028 + rsfS NOLOHH_23730 0.45 -0.4
4,671,028 + rsfS NOLOHH_23730 0.45 +1.0
4,671,272 + rlmH NOLOHH_23735 0.14 +0.4
4,671,504 + rlmH NOLOHH_23735 0.64 +0.4
4,671,541 + rlmH NOLOHH_23735 0.72 +1.6
4,671,674 + -0.8

Or see this region's nucleotide sequence