Experiment: Bas67
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Met_trna and Leu_trna are separated by 8 nucleotides Leu_trna and Gln_trna are separated by 23 nucleotides Gln_trna and Gln_trna are separated by 34 nucleotides Gln_trna and Met_trna are separated by 15 nucleotides Met_trna and Gln_trna are separated by 48 nucleotides Gln_trna and Gln_trna are separated by 37 nucleotides Gln_trna and ubiF are separated by 153 nucleotides ubiF and miaB are separated by 145 nucleotides
NOLOHH_23565: Met_trna - tRNA-Met(cat), at 4,641,975 to 4,642,051
_trna
NOLOHH_23570: Leu_trna - tRNA-Leu(tag), at 4,642,060 to 4,642,144
_trna
NOLOHH_23575: Gln_trna - tRNA-Gln(ttg), at 4,642,168 to 4,642,242
_trna
NOLOHH_23580: Gln_trna - tRNA-Gln(ttg), at 4,642,277 to 4,642,351
_trna
NOLOHH_23585: Met_trna - tRNA-Met(cat), at 4,642,367 to 4,642,443
_trna
NOLOHH_23590: Gln_trna - tRNA-Gln(ctg), at 4,642,492 to 4,642,566
_trna
NOLOHH_23595: Gln_trna - tRNA-Gln(ctg), at 4,642,604 to 4,642,678
_trna
NOLOHH_23600: ubiF - 3-demethoxyubiquinol 3-hydroxylase, at 4,642,832 to 4,644,007
ubiF
NOLOHH_23605: miaB - tRNA (N6-isopentenyl adenosine(37)-C2)-methylthiotransferase MiaB, at 4,644,153 to 4,645,577
miaB
Position (kb)
4642
4643
4644
4645 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 4641.840 kb on - strand at 4641.842 kb on - strand at 4642.254 kb on + strand at 4642.570 kb on + strand at 4642.583 kb on + strand at 4642.583 kb on + strand at 4642.583 kb on + strand at 4642.583 kb on + strand at 4642.590 kb on + strand at 4642.590 kb on + strand at 4642.682 kb on + strand at 4642.828 kb on - strand at 4642.828 kb on - strand at 4642.828 kb on - strand at 4642.828 kb on - strand at 4642.834 kb on - strand at 4642.849 kb on - strand at 4642.849 kb on - strand at 4642.849 kb on - strand at 4644.030 kb on - strand at 4644.030 kb on - strand at 4644.030 kb on - strand at 4644.037 kb on - strand at 4644.037 kb on - strand at 4644.079 kb on - strand at 4644.079 kb on - strand at 4644.104 kb on + strand at 4644.104 kb on + strand at 4644.104 kb on + strand at 4644.105 kb on - strand at 4644.105 kb on - strand at 4644.105 kb on - strand at 4644.105 kb on - strand at 4644.105 kb on - strand at 4644.109 kb on + strand at 4644.133 kb on + strand at 4644.133 kb on + strand at 4644.171 kb on - strand at 4644.359 kb on - strand, within miaB at 4644.444 kb on + strand, within miaB at 4644.445 kb on - strand, within miaB at 4644.529 kb on + strand, within miaB at 4644.534 kb on - strand, within miaB at 4644.627 kb on - strand, within miaB at 4644.630 kb on + strand, within miaB at 4644.630 kb on + strand, within miaB at 4644.630 kb on + strand, within miaB at 4644.631 kb on - strand, within miaB at 4644.655 kb on - strand, within miaB at 4644.860 kb on + strand, within miaB at 4644.860 kb on + strand, within miaB
Per-strain Table
Position Strand Gene LocusTag Fraction Bas67 remove 4,641,840 - +1.4 4,641,842 - -0.3 4,642,254 + -0.6 4,642,570 + -0.2 4,642,583 + -0.8 4,642,583 + +0.3 4,642,583 + -1.7 4,642,583 + -0.1 4,642,590 + -0.5 4,642,590 + -0.8 4,642,682 + +0.2 4,642,828 - -0.4 4,642,828 - -0.6 4,642,828 - +0.2 4,642,828 - -1.1 4,642,834 - -1.4 4,642,849 - +0.2 4,642,849 - -0.4 4,642,849 - +2.4 4,644,030 - +1.1 4,644,030 - +0.2 4,644,030 - +1.0 4,644,037 - +0.1 4,644,037 - -0.2 4,644,079 - +2.0 4,644,079 - -0.6 4,644,104 + +2.8 4,644,104 + +1.1 4,644,104 + -1.3 4,644,105 - +2.0 4,644,105 - -1.9 4,644,105 - +0.1 4,644,105 - +2.8 4,644,105 - +0.9 4,644,109 + +1.1 4,644,133 + +0.1 4,644,133 + -1.1 4,644,171 - +0.8 4,644,359 - miaB NOLOHH_23605 0.14 -0.4 4,644,444 + miaB NOLOHH_23605 0.20 -1.9 4,644,445 - miaB NOLOHH_23605 0.20 +0.1 4,644,529 + miaB NOLOHH_23605 0.26 -1.1 4,644,534 - miaB NOLOHH_23605 0.27 -1.4 4,644,627 - miaB NOLOHH_23605 0.33 +0.4 4,644,630 + miaB NOLOHH_23605 0.33 -1.4 4,644,630 + miaB NOLOHH_23605 0.33 +0.9 4,644,630 + miaB NOLOHH_23605 0.33 +0.4 4,644,631 - miaB NOLOHH_23605 0.34 +0.2 4,644,655 - miaB NOLOHH_23605 0.35 -0.8 4,644,860 + miaB NOLOHH_23605 0.50 -1.6 4,644,860 + miaB NOLOHH_23605 0.50 -1.9
Or see this region's nucleotide sequence