Strain Fitness in Escherichia coli ECOR27 around NOLOHH_23600

Experiment: Bas67

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMet_trna and Leu_trna are separated by 8 nucleotidesLeu_trna and Gln_trna are separated by 23 nucleotidesGln_trna and Gln_trna are separated by 34 nucleotidesGln_trna and Met_trna are separated by 15 nucleotidesMet_trna and Gln_trna are separated by 48 nucleotidesGln_trna and Gln_trna are separated by 37 nucleotidesGln_trna and ubiF are separated by 153 nucleotidesubiF and miaB are separated by 145 nucleotides NOLOHH_23565: Met_trna - tRNA-Met(cat), at 4,641,975 to 4,642,051 _trna NOLOHH_23570: Leu_trna - tRNA-Leu(tag), at 4,642,060 to 4,642,144 _trna NOLOHH_23575: Gln_trna - tRNA-Gln(ttg), at 4,642,168 to 4,642,242 _trna NOLOHH_23580: Gln_trna - tRNA-Gln(ttg), at 4,642,277 to 4,642,351 _trna NOLOHH_23585: Met_trna - tRNA-Met(cat), at 4,642,367 to 4,642,443 _trna NOLOHH_23590: Gln_trna - tRNA-Gln(ctg), at 4,642,492 to 4,642,566 _trna NOLOHH_23595: Gln_trna - tRNA-Gln(ctg), at 4,642,604 to 4,642,678 _trna NOLOHH_23600: ubiF - 3-demethoxyubiquinol 3-hydroxylase, at 4,642,832 to 4,644,007 ubiF NOLOHH_23605: miaB - tRNA (N6-isopentenyl adenosine(37)-C2)-methylthiotransferase MiaB, at 4,644,153 to 4,645,577 miaB Position (kb) 4642 4643 4644 4645Strain fitness (log2 ratio) -2 -1 0 1 2 3at 4641.840 kb on - strandat 4641.842 kb on - strandat 4642.254 kb on + strandat 4642.570 kb on + strandat 4642.583 kb on + strandat 4642.583 kb on + strandat 4642.583 kb on + strandat 4642.583 kb on + strandat 4642.590 kb on + strandat 4642.590 kb on + strandat 4642.682 kb on + strandat 4642.828 kb on - strandat 4642.828 kb on - strandat 4642.828 kb on - strandat 4642.828 kb on - strandat 4642.834 kb on - strandat 4642.849 kb on - strandat 4642.849 kb on - strandat 4642.849 kb on - strandat 4644.030 kb on - strandat 4644.030 kb on - strandat 4644.030 kb on - strandat 4644.037 kb on - strandat 4644.037 kb on - strandat 4644.079 kb on - strandat 4644.079 kb on - strandat 4644.104 kb on + strandat 4644.104 kb on + strandat 4644.104 kb on + strandat 4644.105 kb on - strandat 4644.105 kb on - strandat 4644.105 kb on - strandat 4644.105 kb on - strandat 4644.105 kb on - strandat 4644.109 kb on + strandat 4644.133 kb on + strandat 4644.133 kb on + strandat 4644.171 kb on - strandat 4644.359 kb on - strand, within miaBat 4644.444 kb on + strand, within miaBat 4644.445 kb on - strand, within miaBat 4644.529 kb on + strand, within miaBat 4644.534 kb on - strand, within miaBat 4644.627 kb on - strand, within miaBat 4644.630 kb on + strand, within miaBat 4644.630 kb on + strand, within miaBat 4644.630 kb on + strand, within miaBat 4644.631 kb on - strand, within miaBat 4644.655 kb on - strand, within miaBat 4644.860 kb on + strand, within miaBat 4644.860 kb on + strand, within miaB

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas67
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4,641,840 - +1.4
4,641,842 - -0.3
4,642,254 + -0.6
4,642,570 + -0.2
4,642,583 + -0.8
4,642,583 + +0.3
4,642,583 + -1.7
4,642,583 + -0.1
4,642,590 + -0.5
4,642,590 + -0.8
4,642,682 + +0.2
4,642,828 - -0.4
4,642,828 - -0.6
4,642,828 - +0.2
4,642,828 - -1.1
4,642,834 - -1.4
4,642,849 - +0.2
4,642,849 - -0.4
4,642,849 - +2.4
4,644,030 - +1.1
4,644,030 - +0.2
4,644,030 - +1.0
4,644,037 - +0.1
4,644,037 - -0.2
4,644,079 - +2.0
4,644,079 - -0.6
4,644,104 + +2.8
4,644,104 + +1.1
4,644,104 + -1.3
4,644,105 - +2.0
4,644,105 - -1.9
4,644,105 - +0.1
4,644,105 - +2.8
4,644,105 - +0.9
4,644,109 + +1.1
4,644,133 + +0.1
4,644,133 + -1.1
4,644,171 - +0.8
4,644,359 - miaB NOLOHH_23605 0.14 -0.4
4,644,444 + miaB NOLOHH_23605 0.20 -1.9
4,644,445 - miaB NOLOHH_23605 0.20 +0.1
4,644,529 + miaB NOLOHH_23605 0.26 -1.1
4,644,534 - miaB NOLOHH_23605 0.27 -1.4
4,644,627 - miaB NOLOHH_23605 0.33 +0.4
4,644,630 + miaB NOLOHH_23605 0.33 -1.4
4,644,630 + miaB NOLOHH_23605 0.33 +0.9
4,644,630 + miaB NOLOHH_23605 0.33 +0.4
4,644,631 - miaB NOLOHH_23605 0.34 +0.2
4,644,655 - miaB NOLOHH_23605 0.35 -0.8
4,644,860 + miaB NOLOHH_23605 0.50 -1.6
4,644,860 + miaB NOLOHH_23605 0.50 -1.9

Or see this region's nucleotide sequence