Strain Fitness in Escherichia coli ECOR27 around NOLOHH_22030

Experiment: Bas67

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntycaI and ihfB are separated by 206 nucleotidesihfB and rpsA are separated by 159 nucleotidesrpsA and cmk are separated by 110 nucleotidescmk and ycaL are separated by 172 nucleotides NOLOHH_22020: ycaI - ComEC family protein, at 4,336,388 to 4,338,652 ycaI NOLOHH_22025: ihfB - integration host factor subunit beta, at 4,338,859 to 4,339,143 ihfB NOLOHH_22030: rpsA - 30S ribosomal protein S1, at 4,339,303 to 4,340,976 rpsA NOLOHH_22035: cmk - (d)CMP kinase, at 4,341,087 to 4,341,770 cmk NOLOHH_22040: ycaL - metallopeptidase YcaL, at 4,341,943 to 4,342,707 ycaL Position (kb) 4339 4340 4341Strain fitness (log2 ratio) -1 0 1 2at 4338.309 kb on + strand, within ycaIat 4338.337 kb on + strand, within ycaIat 4338.338 kb on - strand, within ycaIat 4338.338 kb on - strand, within ycaIat 4338.338 kb on - strand, within ycaIat 4338.350 kb on + strand, within ycaIat 4338.351 kb on - strand, within ycaIat 4338.505 kb on + strandat 4338.507 kb on + strandat 4338.529 kb on + strandat 4338.629 kb on + strandat 4338.867 kb on - strandat 4338.958 kb on - strand, within ihfBat 4339.220 kb on - strandat 4341.805 kb on - strandat 4341.873 kb on + strandat 4341.889 kb on + strandat 4341.900 kb on + strandat 4341.901 kb on - strandat 4341.941 kb on - strandat 4341.944 kb on + strandat 4341.945 kb on - strandat 4341.948 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas67
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4,338,309 + ycaI NOLOHH_22020 0.85 +0.1
4,338,337 + ycaI NOLOHH_22020 0.86 -0.1
4,338,338 - ycaI NOLOHH_22020 0.86 +2.3
4,338,338 - ycaI NOLOHH_22020 0.86 +0.3
4,338,338 - ycaI NOLOHH_22020 0.86 +0.3
4,338,350 + ycaI NOLOHH_22020 0.87 -0.7
4,338,351 - ycaI NOLOHH_22020 0.87 +0.3
4,338,505 + -0.7
4,338,507 + +0.3
4,338,529 + +0.6
4,338,629 + -0.2
4,338,867 - -0.9
4,338,958 - ihfB NOLOHH_22025 0.35 -1.2
4,339,220 - +0.8
4,341,805 - -1.1
4,341,873 + -0.9
4,341,889 + -0.4
4,341,900 + +1.9
4,341,901 - +0.9
4,341,941 - -0.1
4,341,944 + +0.3
4,341,945 - -0.7
4,341,948 + -0.4

Or see this region's nucleotide sequence