Strain Fitness in Escherichia coli ECOR27 around NOLOHH_22000

Experiment: Bas67

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntycbJ and kdsB are separated by 153 nucleotideskdsB and ycaR overlap by 4 nucleotidesycaR and ycaQ are separated by 51 nucleotides NOLOHH_21990: ycbJ - Uncharacterized protein YcbJ, at 4,330,327 to 4,331,220 ycbJ NOLOHH_21995: kdsB - 3-deoxy-manno-octulosonate cytidylyltransferase, at 4,331,374 to 4,332,120 kdsB NOLOHH_22000: ycaR - protein YcaR, at 4,332,117 to 4,332,299 ycaR NOLOHH_22005: ycaQ - Uncharacterized protein YcaQ, at 4,332,351 to 4,333,583 ycaQ Position (kb) 4332 4333Strain fitness (log2 ratio) -2 -1 0 1at 4331.167 kb on - strandat 4331.236 kb on - strandat 4331.267 kb on - strandat 4331.376 kb on - strandat 4332.333 kb on - strandat 4332.333 kb on - strandat 4332.367 kb on - strandat 4332.528 kb on - strand, within ycaQat 4332.580 kb on - strand, within ycaQat 4332.594 kb on + strand, within ycaQat 4332.597 kb on + strand, within ycaQat 4332.644 kb on - strand, within ycaQat 4332.644 kb on - strand, within ycaQat 4332.663 kb on - strand, within ycaQat 4332.895 kb on + strand, within ycaQat 4333.080 kb on - strand, within ycaQat 4333.198 kb on - strand, within ycaQ

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas67
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4,331,167 - +0.3
4,331,236 - +1.1
4,331,267 - +0.7
4,331,376 - +0.3
4,332,333 - -0.2
4,332,333 - +1.3
4,332,367 - -2.0
4,332,528 - ycaQ NOLOHH_22005 0.14 -1.0
4,332,580 - ycaQ NOLOHH_22005 0.19 +0.8
4,332,594 + ycaQ NOLOHH_22005 0.20 -0.3
4,332,597 + ycaQ NOLOHH_22005 0.20 +1.7
4,332,644 - ycaQ NOLOHH_22005 0.24 +1.1
4,332,644 - ycaQ NOLOHH_22005 0.24 -0.7
4,332,663 - ycaQ NOLOHH_22005 0.25 -0.7
4,332,895 + ycaQ NOLOHH_22005 0.44 -0.7
4,333,080 - ycaQ NOLOHH_22005 0.59 +0.2
4,333,198 - ycaQ NOLOHH_22005 0.69 -1.5

Or see this region's nucleotide sequence