Experiment: Bas67
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cmoM and elyC are separated by 135 nucleotides elyC and ycbJ overlap by 24 nucleotides ycbJ and kdsB are separated by 153 nucleotides kdsB and ycaR overlap by 4 nucleotides
NOLOHH_21980: cmoM - tRNA uridine 5-oxyacetic acid(34) methyltransferase CmoM, at 4,328,650 to 4,329,435
cmoM
NOLOHH_21985: elyC - envelope biogenesis factor ElyC, at 4,329,571 to 4,330,350
elyC
NOLOHH_21990: ycbJ - Uncharacterized protein YcbJ, at 4,330,327 to 4,331,220
ycbJ
NOLOHH_21995: kdsB - 3-deoxy-manno-octulosonate cytidylyltransferase, at 4,331,374 to 4,332,120
kdsB
NOLOHH_22000: ycaR - protein YcaR, at 4,332,117 to 4,332,299
ycaR
Position (kb) 4330
4331
4332 Strain fitness (log2 ratio) -2
-1
0
1
2 at 4329.384 kb on - strand at 4329.520 kb on + strand at 4329.521 kb on - strand at 4329.521 kb on - strand at 4329.543 kb on + strand at 4329.543 kb on + strand at 4329.543 kb on + strand at 4329.544 kb on - strand at 4329.544 kb on - strand at 4329.544 kb on - strand at 4329.544 kb on - strand at 4329.544 kb on - strand at 4329.544 kb on - strand at 4329.544 kb on - strand at 4329.544 kb on - strand at 4329.544 kb on - strand at 4329.544 kb on - strand at 4329.545 kb on + strand at 4329.545 kb on + strand at 4329.632 kb on + strand at 4329.632 kb on + strand at 4329.636 kb on + strand at 4329.636 kb on + strand at 4329.700 kb on - strand, within elyC at 4329.792 kb on + strand, within elyC at 4329.792 kb on + strand, within elyC at 4329.793 kb on - strand, within elyC at 4329.811 kb on + strand, within elyC at 4329.811 kb on + strand, within elyC at 4329.837 kb on + strand, within elyC at 4329.837 kb on + strand, within elyC at 4329.872 kb on + strand, within elyC at 4329.906 kb on + strand, within elyC at 4329.914 kb on + strand, within elyC at 4329.981 kb on + strand, within elyC at 4329.992 kb on + strand, within elyC at 4329.992 kb on + strand, within elyC at 4329.992 kb on + strand, within elyC at 4329.992 kb on + strand, within elyC at 4330.038 kb on + strand, within elyC at 4330.038 kb on + strand, within elyC at 4330.176 kb on - strand, within elyC at 4330.427 kb on + strand, within ycbJ at 4330.427 kb on + strand, within ycbJ at 4330.428 kb on - strand, within ycbJ at 4330.517 kb on - strand, within ycbJ at 4330.579 kb on + strand, within ycbJ at 4330.666 kb on + strand, within ycbJ at 4330.732 kb on - strand, within ycbJ at 4330.756 kb on + strand, within ycbJ at 4330.806 kb on + strand, within ycbJ at 4331.076 kb on + strand, within ycbJ at 4331.167 kb on - strand at 4331.236 kb on - strand at 4331.267 kb on - strand at 4331.376 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas67 remove 4,329,384 - +0.3 4,329,520 + +1.1 4,329,521 - +0.3 4,329,521 - -0.7 4,329,543 + -0.7 4,329,543 + -0.1 4,329,543 + -1.5 4,329,544 - -0.2 4,329,544 - +1.6 4,329,544 - -0.7 4,329,544 - +0.3 4,329,544 - +0.3 4,329,544 - +2.3 4,329,544 - +0.9 4,329,544 - +0.8 4,329,544 - +1.3 4,329,544 - -0.2 4,329,545 + +0.3 4,329,545 + +1.3 4,329,632 + +1.1 4,329,632 + +0.5 4,329,636 + +0.7 4,329,636 + +1.3 4,329,700 - elyC NOLOHH_21985 0.17 +1.3 4,329,792 + elyC NOLOHH_21985 0.28 +1.3 4,329,792 + elyC NOLOHH_21985 0.28 +0.8 4,329,793 - elyC NOLOHH_21985 0.28 +1.3 4,329,811 + elyC NOLOHH_21985 0.31 +0.3 4,329,811 + elyC NOLOHH_21985 0.31 +0.8 4,329,837 + elyC NOLOHH_21985 0.34 -1.2 4,329,837 + elyC NOLOHH_21985 0.34 -0.4 4,329,872 + elyC NOLOHH_21985 0.39 -0.4 4,329,906 + elyC NOLOHH_21985 0.43 -0.7 4,329,914 + elyC NOLOHH_21985 0.44 -0.7 4,329,981 + elyC NOLOHH_21985 0.53 -0.4 4,329,992 + elyC NOLOHH_21985 0.54 -1.7 4,329,992 + elyC NOLOHH_21985 0.54 +0.3 4,329,992 + elyC NOLOHH_21985 0.54 +0.7 4,329,992 + elyC NOLOHH_21985 0.54 +1.9 4,330,038 + elyC NOLOHH_21985 0.60 -0.1 4,330,038 + elyC NOLOHH_21985 0.60 -0.5 4,330,176 - elyC NOLOHH_21985 0.78 +0.3 4,330,427 + ycbJ NOLOHH_21990 0.11 -1.2 4,330,427 + ycbJ NOLOHH_21990 0.11 -0.9 4,330,428 - ycbJ NOLOHH_21990 0.11 -1.2 4,330,517 - ycbJ NOLOHH_21990 0.21 -2.1 4,330,579 + ycbJ NOLOHH_21990 0.28 -0.2 4,330,666 + ycbJ NOLOHH_21990 0.38 +0.3 4,330,732 - ycbJ NOLOHH_21990 0.45 +0.3 4,330,756 + ycbJ NOLOHH_21990 0.48 +0.3 4,330,806 + ycbJ NOLOHH_21990 0.54 +0.3 4,331,076 + ycbJ NOLOHH_21990 0.84 -0.1 4,331,167 - +0.3 4,331,236 - +1.1 4,331,267 - +0.7 4,331,376 - +0.3
Or see this region's nucleotide sequence