Strain Fitness in Escherichia coli ECOR27 around NOLOHH_21725

Experiment: Bas67

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nthyaA and Ser_trna are separated by 426 nucleotidesSer_trna and yccA are separated by 206 nucleotidesyccA and tusE are separated by 90 nucleotidestusE and yccX overlap by 4 nucleotidesyccX and rlmI are separated by 109 nucleotides NOLOHH_21715: hyaA - hydrogenase 1 small subunit, at 4,269,549 to 4,270,667 hyaA NOLOHH_21720: Ser_trna - tRNA-Ser(tga), at 4,271,094 to 4,271,181 _trna NOLOHH_21725: yccA - FtsH protease modulator YccA, at 4,271,388 to 4,272,047 yccA NOLOHH_21730: tusE - sulfurtransferase TusE, at 4,272,138 to 4,272,467 tusE NOLOHH_21735: yccX - acylphosphatase, at 4,272,464 to 4,272,742 yccX NOLOHH_21740: rlmI - 23S rRNA (cytosine(1962)-C(5))-methyltransferase RlmI, at 4,272,852 to 4,274,027 rlmI Position (kb) 4271 4272 4273Strain fitness (log2 ratio) -2 -1 0 1 2 3at 4270.395 kb on - strand, within hyaAat 4270.567 kb on - strandat 4270.593 kb on - strandat 4270.597 kb on + strandat 4270.646 kb on - strandat 4270.819 kb on - strandat 4270.828 kb on - strandat 4270.900 kb on + strandat 4271.184 kb on + strandat 4271.184 kb on + strandat 4271.201 kb on + strandat 4271.201 kb on + strandat 4271.201 kb on + strandat 4271.201 kb on + strandat 4271.250 kb on + strandat 4271.333 kb on + strandat 4271.359 kb on + strandat 4271.363 kb on + strandat 4271.421 kb on - strandat 4271.441 kb on + strandat 4271.565 kb on + strand, within yccAat 4271.676 kb on + strand, within yccAat 4271.676 kb on + strand, within yccAat 4271.677 kb on - strand, within yccAat 4271.702 kb on - strand, within yccAat 4271.754 kb on + strand, within yccAat 4271.755 kb on - strand, within yccAat 4271.777 kb on + strand, within yccAat 4271.831 kb on + strand, within yccAat 4271.958 kb on + strand, within yccAat 4271.982 kb on + strandat 4271.982 kb on + strandat 4271.994 kb on + strandat 4271.994 kb on + strandat 4271.994 kb on + strandat 4272.080 kb on + strandat 4272.080 kb on + strandat 4272.127 kb on + strandat 4272.465 kb on + strandat 4272.466 kb on - strandat 4272.570 kb on - strand, within yccXat 4272.670 kb on - strand, within yccXat 4272.712 kb on - strand, within yccXat 4272.832 kb on - strandat 4272.832 kb on - strandat 4272.844 kb on + strandat 4272.845 kb on - strandat 4272.845 kb on - strandat 4272.845 kb on - strandat 4272.851 kb on - strandat 4272.932 kb on + strandat 4272.999 kb on + strand, within rlmI

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas67
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4,270,395 - hyaA NOLOHH_21715 0.76 +0.9
4,270,567 - +0.8
4,270,593 - +3.1
4,270,597 + -1.2
4,270,646 - +0.0
4,270,819 - -0.5
4,270,828 - -0.0
4,270,900 + +0.3
4,271,184 + -2.5
4,271,184 + +0.3
4,271,201 + +0.7
4,271,201 + -1.2
4,271,201 + +0.6
4,271,201 + -0.3
4,271,250 + -0.3
4,271,333 + -0.8
4,271,359 + +0.3
4,271,363 + -0.2
4,271,421 - -0.7
4,271,441 + -1.2
4,271,565 + yccA NOLOHH_21725 0.27 -0.1
4,271,676 + yccA NOLOHH_21725 0.44 +1.3
4,271,676 + yccA NOLOHH_21725 0.44 +0.9
4,271,677 - yccA NOLOHH_21725 0.44 -0.7
4,271,702 - yccA NOLOHH_21725 0.48 +0.3
4,271,754 + yccA NOLOHH_21725 0.55 +0.3
4,271,755 - yccA NOLOHH_21725 0.56 +0.9
4,271,777 + yccA NOLOHH_21725 0.59 +0.3
4,271,831 + yccA NOLOHH_21725 0.67 +0.1
4,271,958 + yccA NOLOHH_21725 0.86 +1.3
4,271,982 + -0.7
4,271,982 + +0.3
4,271,994 + -0.2
4,271,994 + -1.1
4,271,994 + -1.7
4,272,080 + +0.3
4,272,080 + +0.5
4,272,127 + -0.7
4,272,465 + +0.3
4,272,466 - -0.7
4,272,570 - yccX NOLOHH_21735 0.38 +1.3
4,272,670 - yccX NOLOHH_21735 0.74 +0.1
4,272,712 - yccX NOLOHH_21735 0.89 -0.4
4,272,832 - -0.8
4,272,832 - +1.6
4,272,844 + +0.3
4,272,845 - +0.3
4,272,845 - +1.1
4,272,845 - +0.6
4,272,851 - +2.9
4,272,932 + -1.7
4,272,999 + rlmI NOLOHH_21740 0.12 +1.3

Or see this region's nucleotide sequence