Strain Fitness in Escherichia coli ECOR27 around NOLOHH_18250

Experiment: Bas67

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntmlc and bioD are separated by 124 nucleotidesbioD and clcB overlap by 48 nucleotides NOLOHH_18245: mlc - DNA-binding transcriptional repressor Mlc, at 3,584,477 to 3,585,697 mlc NOLOHH_18250: bioD - dethiobiotin synthase, at 3,585,822 to 3,586,517 bioD NOLOHH_18255: clcB - voltage-gated ClC-type chloride channel ClcB, at 3,586,470 to 3,587,726 clcB Position (kb) 3585 3586 3587Strain fitness (log2 ratio) -2 -1 0 1 2at 3584.967 kb on + strand, within mlcat 3584.994 kb on + strand, within mlcat 3585.180 kb on + strand, within mlcat 3585.345 kb on + strand, within mlcat 3585.792 kb on + strandat 3586.461 kb on + strandat 3586.461 kb on + strandat 3586.461 kb on + strandat 3586.597 kb on - strand, within clcBat 3586.677 kb on - strand, within clcBat 3586.702 kb on - strand, within clcBat 3586.720 kb on + strand, within clcBat 3586.721 kb on - strand, within clcBat 3587.290 kb on - strand, within clcB

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas67
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3,584,967 + mlc NOLOHH_18245 0.40 +2.2
3,584,994 + mlc NOLOHH_18245 0.42 +1.2
3,585,180 + mlc NOLOHH_18245 0.58 +0.2
3,585,345 + mlc NOLOHH_18245 0.71 -0.4
3,585,792 + +1.2
3,586,461 + +1.0
3,586,461 + -0.8
3,586,461 + -1.8
3,586,597 - clcB NOLOHH_18255 0.10 +0.6
3,586,677 - clcB NOLOHH_18255 0.16 +0.2
3,586,702 - clcB NOLOHH_18255 0.18 +0.5
3,586,720 + clcB NOLOHH_18255 0.20 -0.5
3,586,721 - clcB NOLOHH_18255 0.20 +1.2
3,587,290 - clcB NOLOHH_18255 0.65 +0.2

Or see this region's nucleotide sequence