Strain Fitness in Escherichia coli ECOR27 around NOLOHH_17665

Experiment: Bas67

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntppsR and ppsA are separated by 331 nucleotidesppsA and fadK are separated by 56 nucleotides NOLOHH_17660: ppsR - posphoenolpyruvate synthetase regulatory kinase/phosphorylase PpsR, at 3,464,939 to 3,465,772 ppsR NOLOHH_17665: ppsA - phosphoenolpyruvate synthase, at 3,466,104 to 3,468,482 ppsA NOLOHH_17670: fadK - medium-chain fatty-acid--CoA ligase, at 3,468,539 to 3,470,185 fadK Position (kb) 3466 3467 3468 3469Strain fitness (log2 ratio) -2 -1 0 1 2at 3465.397 kb on - strand, within ppsRat 3465.995 kb on + strandat 3466.038 kb on + strandat 3466.165 kb on + strandat 3466.437 kb on + strand, within ppsAat 3466.505 kb on + strand, within ppsAat 3466.835 kb on + strand, within ppsAat 3467.024 kb on - strand, within ppsAat 3467.510 kb on + strand, within ppsAat 3468.055 kb on + strand, within ppsAat 3468.342 kb on + strandat 3468.342 kb on + strandat 3468.480 kb on + strandat 3468.945 kb on + strand, within fadKat 3469.304 kb on + strand, within fadKat 3469.304 kb on + strand, within fadKat 3469.320 kb on + strand, within fadKat 3469.427 kb on + strand, within fadK

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas67
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3,465,397 - ppsR NOLOHH_17660 0.55 -0.8
3,465,995 + -1.2
3,466,038 + +0.2
3,466,165 + -0.5
3,466,437 + ppsA NOLOHH_17665 0.14 +1.8
3,466,505 + ppsA NOLOHH_17665 0.17 +1.8
3,466,835 + ppsA NOLOHH_17665 0.31 +0.2
3,467,024 - ppsA NOLOHH_17665 0.39 -1.0
3,467,510 + ppsA NOLOHH_17665 0.59 -0.4
3,468,055 + ppsA NOLOHH_17665 0.82 +0.2
3,468,342 + +0.5
3,468,342 + +1.0
3,468,480 + -2.1
3,468,945 + fadK NOLOHH_17670 0.25 +0.6
3,469,304 + fadK NOLOHH_17670 0.46 +0.8
3,469,304 + fadK NOLOHH_17670 0.46 +0.2
3,469,320 + fadK NOLOHH_17670 0.47 -0.1
3,469,427 + fadK NOLOHH_17670 0.54 +0.2

Or see this region's nucleotide sequence