Experiment: Bas67
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pdeD and sdaA are separated by 130 nucleotides sdaA and nudL are separated by 183 nucleotides nudL and pabB are separated by 3 nucleotides
NOLOHH_17065: pdeD - putative cyclic di-GMP phosphodiesterase PdeD, at 3,349,923 to 3,351,521
pdeD
NOLOHH_17070: sdaA - L-serine ammonia-lyase, at 3,351,652 to 3,353,016
sdaA
NOLOHH_17075: nudL - CoA pyrophosphatase, at 3,353,200 to 3,353,778
nudL
NOLOHH_17080: pabB - aminodeoxychorismate synthase component 1, at 3,353,782 to 3,355,143
pabB
Position (kb)
3351
3352
3353
3354 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3350.769 kb on + strand, within pdeD at 3350.803 kb on + strand, within pdeD at 3351.009 kb on + strand, within pdeD at 3351.047 kb on + strand, within pdeD at 3351.047 kb on + strand, within pdeD at 3351.264 kb on + strand, within pdeD at 3351.360 kb on + strand, within pdeD at 3351.400 kb on - strand at 3351.490 kb on + strand at 3351.490 kb on + strand at 3351.576 kb on + strand at 3351.577 kb on - strand at 3351.586 kb on + strand at 3351.589 kb on + strand at 3351.612 kb on + strand at 3351.651 kb on - strand at 3351.651 kb on - strand at 3352.024 kb on - strand, within sdaA at 3352.024 kb on - strand, within sdaA at 3352.041 kb on - strand, within sdaA at 3352.069 kb on - strand, within sdaA at 3352.083 kb on - strand, within sdaA at 3352.083 kb on - strand, within sdaA at 3352.119 kb on - strand, within sdaA at 3352.119 kb on - strand, within sdaA at 3352.277 kb on - strand, within sdaA at 3352.360 kb on - strand, within sdaA at 3352.395 kb on - strand, within sdaA at 3352.479 kb on - strand, within sdaA at 3352.567 kb on - strand, within sdaA at 3353.063 kb on - strand at 3353.073 kb on - strand at 3353.078 kb on - strand at 3353.078 kb on - strand at 3353.102 kb on - strand at 3353.185 kb on + strand at 3353.186 kb on - strand at 3353.195 kb on + strand at 3353.196 kb on - strand at 3353.324 kb on - strand, within nudL at 3353.348 kb on - strand, within nudL at 3353.348 kb on - strand, within nudL at 3353.698 kb on + strand, within nudL at 3353.783 kb on + strand at 3353.784 kb on - strand at 3353.801 kb on - strand at 3353.812 kb on - strand at 3353.835 kb on - strand at 3353.885 kb on + strand at 3353.888 kb on - strand at 3353.888 kb on - strand at 3353.888 kb on - strand at 3353.888 kb on - strand at 3353.888 kb on - strand at 3353.888 kb on - strand at 3353.923 kb on - strand, within pabB at 3353.958 kb on - strand, within pabB at 3353.983 kb on - strand, within pabB
Per-strain Table
Position Strand Gene LocusTag Fraction Bas67 remove 3,350,769 + pdeD NOLOHH_17065 0.53 +0.2 3,350,803 + pdeD NOLOHH_17065 0.55 -0.3 3,351,009 + pdeD NOLOHH_17065 0.68 +1.2 3,351,047 + pdeD NOLOHH_17065 0.70 +0.2 3,351,047 + pdeD NOLOHH_17065 0.70 +1.2 3,351,264 + pdeD NOLOHH_17065 0.84 -1.6 3,351,360 + pdeD NOLOHH_17065 0.90 +1.2 3,351,400 - -0.0 3,351,490 + -0.3 3,351,490 + -0.8 3,351,576 + -1.3 3,351,577 - +0.8 3,351,586 + -0.4 3,351,589 + -0.8 3,351,612 + -1.4 3,351,651 - -1.1 3,351,651 - +1.8 3,352,024 - sdaA NOLOHH_17070 0.27 +0.2 3,352,024 - sdaA NOLOHH_17070 0.27 +1.6 3,352,041 - sdaA NOLOHH_17070 0.28 +1.4 3,352,069 - sdaA NOLOHH_17070 0.31 +1.2 3,352,083 - sdaA NOLOHH_17070 0.32 +0.0 3,352,083 - sdaA NOLOHH_17070 0.32 +0.8 3,352,119 - sdaA NOLOHH_17070 0.34 -1.6 3,352,119 - sdaA NOLOHH_17070 0.34 +0.1 3,352,277 - sdaA NOLOHH_17070 0.46 +0.5 3,352,360 - sdaA NOLOHH_17070 0.52 +0.2 3,352,395 - sdaA NOLOHH_17070 0.54 +0.2 3,352,479 - sdaA NOLOHH_17070 0.61 -2.4 3,352,567 - sdaA NOLOHH_17070 0.67 +1.8 3,353,063 - +0.2 3,353,073 - +0.2 3,353,078 - +0.6 3,353,078 - +1.2 3,353,102 - -1.4 3,353,185 + +0.2 3,353,186 - +1.2 3,353,195 + +0.2 3,353,196 - -0.2 3,353,324 - nudL NOLOHH_17075 0.21 +0.2 3,353,348 - nudL NOLOHH_17075 0.26 -2.1 3,353,348 - nudL NOLOHH_17075 0.26 +1.2 3,353,698 + nudL NOLOHH_17075 0.86 +0.5 3,353,783 + -1.8 3,353,784 - -0.4 3,353,801 - +0.8 3,353,812 - -0.8 3,353,835 - -1.2 3,353,885 + -0.5 3,353,888 - +0.2 3,353,888 - -1.4 3,353,888 - +1.2 3,353,888 - -1.0 3,353,888 - -0.5 3,353,888 - -2.1 3,353,923 - pabB NOLOHH_17080 0.10 -0.1 3,353,958 - pabB NOLOHH_17080 0.13 -0.4 3,353,983 - pabB NOLOHH_17080 0.15 -0.2
Or see this region's nucleotide sequence