Experiment: Bas67
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt uvrC and pgsA are separated by 56 nucleotides pgsA and Gly_trna are separated by 151 nucleotides Gly_trna and Cys_trna are separated by 54 nucleotides Cys_trna and Leu_trna are separated by 12 nucleotides Leu_trna and yecA are separated by 195 nucleotides
NOLOHH_16445: uvrC - excinuclease ABC subunit UvrC, at 3,236,594 to 3,238,426
uvrC
NOLOHH_16450: pgsA - CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase, at 3,238,483 to 3,239,031
pgsA
NOLOHH_16455: Gly_trna - tRNA-Gly(gcc), at 3,239,183 to 3,239,258
_trna
NOLOHH_16460: Cys_trna - tRNA-Cys(gca), at 3,239,313 to 3,239,386
_trna
NOLOHH_16465: Leu_trna - tRNA-Leu(taa), at 3,239,399 to 3,239,485
_trna
NOLOHH_16470: yecA - UPF0149 family protein YecA, at 3,239,681 to 3,240,346
yecA
Position (kb)
3239
3240 Strain fitness (log2 ratio)
-1
0
1
2 at 3238.231 kb on + strand, within uvrC at 3238.316 kb on + strand at 3238.318 kb on + strand at 3238.468 kb on + strand at 3239.640 kb on - strand at 3240.042 kb on - strand, within yecA at 3240.183 kb on + strand, within yecA at 3240.213 kb on + strand, within yecA at 3240.213 kb on + strand, within yecA
Per-strain Table
Position Strand Gene LocusTag Fraction Bas67 remove 3,238,231 + uvrC NOLOHH_16445 0.89 -0.7 3,238,316 + -0.3 3,238,318 + +2.3 3,238,468 + -1.7 3,239,640 - +0.3 3,240,042 - yecA NOLOHH_16470 0.54 +0.3 3,240,183 + yecA NOLOHH_16470 0.75 -1.1 3,240,213 + yecA NOLOHH_16470 0.80 +0.3 3,240,213 + yecA NOLOHH_16470 0.80 -1.5
Or see this region's nucleotide sequence