Strain Fitness in Escherichia coli ECOR27 around NOLOHH_16450

Experiment: Bas67

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntuvrC and pgsA are separated by 56 nucleotidespgsA and Gly_trna are separated by 151 nucleotidesGly_trna and Cys_trna are separated by 54 nucleotidesCys_trna and Leu_trna are separated by 12 nucleotidesLeu_trna and yecA are separated by 195 nucleotides NOLOHH_16445: uvrC - excinuclease ABC subunit UvrC, at 3,236,594 to 3,238,426 uvrC NOLOHH_16450: pgsA - CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase, at 3,238,483 to 3,239,031 pgsA NOLOHH_16455: Gly_trna - tRNA-Gly(gcc), at 3,239,183 to 3,239,258 _trna NOLOHH_16460: Cys_trna - tRNA-Cys(gca), at 3,239,313 to 3,239,386 _trna NOLOHH_16465: Leu_trna - tRNA-Leu(taa), at 3,239,399 to 3,239,485 _trna NOLOHH_16470: yecA - UPF0149 family protein YecA, at 3,239,681 to 3,240,346 yecA Position (kb) 3238 3239 3240Strain fitness (log2 ratio) -1 0 1 2at 3237.617 kb on - strand, within uvrCat 3237.883 kb on + strand, within uvrCat 3237.909 kb on - strand, within uvrCat 3237.964 kb on + strand, within uvrCat 3238.071 kb on + strand, within uvrCat 3238.147 kb on + strand, within uvrCat 3238.147 kb on + strand, within uvrCat 3238.180 kb on + strand, within uvrCat 3238.231 kb on + strand, within uvrCat 3238.316 kb on + strandat 3238.318 kb on + strandat 3238.468 kb on + strandat 3239.640 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas67
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3,237,617 - uvrC NOLOHH_16445 0.56 +1.3
3,237,883 + uvrC NOLOHH_16445 0.70 +0.8
3,237,909 - uvrC NOLOHH_16445 0.72 +0.3
3,237,964 + uvrC NOLOHH_16445 0.75 +1.0
3,238,071 + uvrC NOLOHH_16445 0.81 +0.3
3,238,147 + uvrC NOLOHH_16445 0.85 +0.5
3,238,147 + uvrC NOLOHH_16445 0.85 -0.7
3,238,180 + uvrC NOLOHH_16445 0.87 +0.3
3,238,231 + uvrC NOLOHH_16445 0.89 -0.7
3,238,316 + -0.3
3,238,318 + +2.3
3,238,468 + -1.7
3,239,640 - +0.3

Or see this region's nucleotide sequence