Experiment: Bas67
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt iscU and iscA are separated by 16 nucleotides iscA and hscB are separated by 95 nucleotides hscB and hscA are separated by 16 nucleotides hscA and fdx are separated by 1 nucleotides fdx and iscX are separated by 11 nucleotides iscX and pepB are separated by 177 nucleotides
NOLOHH_13295: iscU - Iron-sulfur cluster assembly scaffold protein IscU, at 2,577,991 to 2,578,377
iscU
NOLOHH_13300: iscA - iron-sulfur cluster assembly protein IscA, at 2,578,394 to 2,578,717
iscA
NOLOHH_13305: hscB - co-chaperone HscB, at 2,578,813 to 2,579,328
hscB
NOLOHH_13310: hscA - Fe-S protein assembly chaperone HscA, at 2,579,345 to 2,581,195
hscA
NOLOHH_13315: fdx - ISC system 2Fe-2S type ferredoxin, at 2,581,197 to 2,581,532
fdx
NOLOHH_13320: iscX - Fe-S cluster assembly protein IscX, at 2,581,544 to 2,581,744
iscX
NOLOHH_13325: pepB - aminopeptidase PepB, at 2,581,922 to 2,583,205
pepB
Position (kb)
2579
2580
2581
2582 Strain fitness (log2 ratio)
-2
-1
0
1 at 2578.353 kb on + strand at 2578.353 kb on + strand at 2578.353 kb on + strand at 2578.393 kb on + strand at 2578.431 kb on + strand, within iscA at 2578.435 kb on + strand, within iscA at 2578.511 kb on + strand, within iscA at 2578.515 kb on + strand, within iscA at 2579.590 kb on + strand, within hscA at 2579.799 kb on + strand, within hscA at 2579.961 kb on - strand, within hscA at 2580.075 kb on + strand, within hscA at 2580.280 kb on - strand, within hscA at 2580.359 kb on - strand, within hscA at 2580.586 kb on + strand, within hscA at 2580.654 kb on + strand, within hscA at 2580.654 kb on + strand, within hscA at 2580.655 kb on - strand, within hscA at 2580.899 kb on + strand, within hscA at 2580.915 kb on + strand, within hscA at 2581.193 kb on + strand at 2581.194 kb on - strand at 2581.508 kb on + strand at 2581.530 kb on + strand at 2581.531 kb on - strand at 2581.540 kb on + strand at 2581.540 kb on + strand at 2581.541 kb on - strand at 2581.552 kb on + strand at 2581.552 kb on + strand at 2581.552 kb on + strand at 2581.553 kb on - strand at 2581.553 kb on - strand at 2581.553 kb on - strand at 2581.553 kb on - strand at 2581.719 kb on + strand, within iscX at 2581.804 kb on + strand at 2581.804 kb on + strand at 2581.804 kb on + strand at 2581.804 kb on + strand at 2581.804 kb on + strand at 2581.804 kb on + strand at 2581.805 kb on - strand at 2581.805 kb on - strand at 2581.805 kb on - strand at 2581.805 kb on - strand at 2581.805 kb on - strand at 2581.805 kb on - strand at 2581.805 kb on - strand at 2581.808 kb on + strand at 2581.808 kb on + strand at 2581.808 kb on + strand at 2581.808 kb on + strand at 2581.808 kb on + strand at 2581.808 kb on + strand at 2581.808 kb on + strand at 2581.809 kb on - strand at 2581.809 kb on - strand at 2581.812 kb on + strand at 2581.812 kb on + strand at 2581.812 kb on + strand at 2581.812 kb on + strand at 2581.812 kb on + strand at 2581.812 kb on + strand at 2581.812 kb on + strand at 2581.813 kb on - strand at 2581.813 kb on - strand at 2581.918 kb on + strand at 2581.919 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas67 remove 2,578,353 + -1.7 2,578,353 + +0.3 2,578,353 + -0.7 2,578,393 + +0.3 2,578,431 + iscA NOLOHH_13300 0.11 +0.3 2,578,435 + iscA NOLOHH_13300 0.13 +0.3 2,578,511 + iscA NOLOHH_13300 0.36 +0.3 2,578,515 + iscA NOLOHH_13300 0.37 -0.1 2,579,590 + hscA NOLOHH_13310 0.13 +0.3 2,579,799 + hscA NOLOHH_13310 0.25 +0.3 2,579,961 - hscA NOLOHH_13310 0.33 +0.3 2,580,075 + hscA NOLOHH_13310 0.39 +0.3 2,580,280 - hscA NOLOHH_13310 0.51 -0.7 2,580,359 - hscA NOLOHH_13310 0.55 +0.3 2,580,586 + hscA NOLOHH_13310 0.67 +0.3 2,580,654 + hscA NOLOHH_13310 0.71 -1.3 2,580,654 + hscA NOLOHH_13310 0.71 +0.3 2,580,655 - hscA NOLOHH_13310 0.71 +0.3 2,580,899 + hscA NOLOHH_13310 0.84 +0.3 2,580,915 + hscA NOLOHH_13310 0.85 +0.3 2,581,193 + -0.7 2,581,194 - +1.3 2,581,508 + +0.3 2,581,530 + -0.3 2,581,531 - +0.3 2,581,540 + -0.4 2,581,540 + +0.7 2,581,541 - -0.3 2,581,552 + -0.5 2,581,552 + +1.1 2,581,552 + -0.3 2,581,553 - -0.7 2,581,553 - -1.7 2,581,553 - -0.2 2,581,553 - -1.5 2,581,719 + iscX NOLOHH_13320 0.87 +1.2 2,581,804 + -0.7 2,581,804 + -0.1 2,581,804 + -0.3 2,581,804 + -0.0 2,581,804 + +0.8 2,581,804 + -0.8 2,581,805 - +0.6 2,581,805 - +0.3 2,581,805 - +0.1 2,581,805 - -1.8 2,581,805 - +0.3 2,581,805 - -0.3 2,581,805 - -1.7 2,581,808 + -1.5 2,581,808 + -0.1 2,581,808 + -0.9 2,581,808 + -1.1 2,581,808 + +0.3 2,581,808 + +0.1 2,581,808 + -0.1 2,581,809 - +0.3 2,581,809 - -0.7 2,581,812 + +0.3 2,581,812 + -0.1 2,581,812 + -0.4 2,581,812 + +0.2 2,581,812 + -1.1 2,581,812 + -0.4 2,581,812 + +0.0 2,581,813 - -1.3 2,581,813 - +1.3 2,581,918 + +1.1 2,581,919 - -0.3
Or see this region's nucleotide sequence