Strain Fitness in Escherichia coli ECOR27 around NOLOHH_12865

Experiment: Bas67

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntrimM and trmD are separated by 30 nucleotidestrmD and rplS are separated by 41 nucleotidesrplS and yfiB are separated by 76 nucleotidesyfiB and dgcN are separated by 15 nucleotides NOLOHH_12855: rimM - ribosome maturation factor RimM, at 2,492,657 to 2,493,205 rimM NOLOHH_12860: trmD - tRNA (guanosine(37)-N1)-methyltransferase TrmD, at 2,493,236 to 2,494,003 trmD NOLOHH_12865: rplS - 50S ribosomal protein L19, at 2,494,045 to 2,494,392 rplS NOLOHH_12870: yfiB - OmpA family protein, at 2,494,469 to 2,494,951 yfiB NOLOHH_12875: dgcN - diguanylate cyclase DgcN, at 2,494,967 to 2,496,193 dgcN Position (kb) 2494 2495Strain fitness (log2 ratio) -2 -1 0 1at 2494.481 kb on - strandat 2494.481 kb on - strandat 2494.481 kb on - strandat 2494.654 kb on - strand, within yfiBat 2494.654 kb on - strand, within yfiBat 2494.668 kb on + strand, within yfiBat 2494.668 kb on + strand, within yfiBat 2494.669 kb on - strand, within yfiBat 2494.669 kb on - strand, within yfiBat 2494.669 kb on - strand, within yfiBat 2494.669 kb on - strand, within yfiBat 2494.803 kb on - strand, within yfiBat 2494.908 kb on - strandat 2494.960 kb on - strandat 2495.011 kb on + strandat 2495.011 kb on + strandat 2495.012 kb on - strandat 2495.012 kb on - strandat 2495.012 kb on - strandat 2495.012 kb on - strandat 2495.012 kb on - strandat 2495.012 kb on - strandat 2495.012 kb on - strandat 2495.012 kb on - strandat 2495.012 kb on - strandat 2495.082 kb on - strandat 2495.180 kb on + strand, within dgcNat 2495.181 kb on - strand, within dgcNat 2495.181 kb on - strand, within dgcNat 2495.320 kb on - strand, within dgcNat 2495.346 kb on - strand, within dgcN

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas67
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2,494,481 - -0.7
2,494,481 - -0.7
2,494,481 - -0.7
2,494,654 - yfiB NOLOHH_12870 0.38 +1.3
2,494,654 - yfiB NOLOHH_12870 0.38 +0.0
2,494,668 + yfiB NOLOHH_12870 0.41 +0.0
2,494,668 + yfiB NOLOHH_12870 0.41 -1.7
2,494,669 - yfiB NOLOHH_12870 0.41 -1.3
2,494,669 - yfiB NOLOHH_12870 0.41 -0.3
2,494,669 - yfiB NOLOHH_12870 0.41 -2.0
2,494,669 - yfiB NOLOHH_12870 0.41 -0.3
2,494,803 - yfiB NOLOHH_12870 0.69 -0.7
2,494,908 - -0.8
2,494,960 - -0.8
2,495,011 + +0.3
2,495,011 + -0.7
2,495,012 - -0.1
2,495,012 - -0.5
2,495,012 - -1.3
2,495,012 - -0.7
2,495,012 - +0.1
2,495,012 - +1.0
2,495,012 - +0.9
2,495,012 - -1.3
2,495,012 - -0.7
2,495,082 - -1.8
2,495,180 + dgcN NOLOHH_12875 0.17 +0.3
2,495,181 - dgcN NOLOHH_12875 0.17 -2.3
2,495,181 - dgcN NOLOHH_12875 0.17 +0.1
2,495,320 - dgcN NOLOHH_12875 0.29 +0.3
2,495,346 - dgcN NOLOHH_12875 0.31 -1.3

Or see this region's nucleotide sequence