Experiment: Bas67
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nrdI and nrdH overlap by 4 nucleotides nrdH and ygaM are separated by 247 nucleotides ygaM and ygaC are separated by 151 nucleotides ygaC and alaE are separated by 36 nucleotides
NOLOHH_12675: nrdI - class Ib ribonucleoside-diphosphate reductase assembly flavoprotein NrdI, at 2,461,423 to 2,461,833
nrdI
NOLOHH_12680: nrdH - glutaredoxin-like protein NrdH, at 2,461,830 to 2,462,075
nrdH
NOLOHH_12685: ygaM - Uncharacterized protein YgaM, at 2,462,323 to 2,462,652
ygaM
NOLOHH_12690: ygaC - DUF2002 domain-containing protein, at 2,462,804 to 2,463,148
ygaC
NOLOHH_12695: alaE - L-alanine exporter AlaE, at 2,463,185 to 2,463,634
alaE
Position (kb)
2462
2463
2464 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2461.817 kb on + strand at 2461.817 kb on + strand at 2461.818 kb on - strand at 2461.899 kb on - strand, within nrdH at 2461.982 kb on + strand, within nrdH at 2461.983 kb on - strand, within nrdH at 2462.186 kb on + strand at 2462.186 kb on + strand at 2462.186 kb on + strand at 2462.187 kb on - strand at 2462.304 kb on - strand at 2462.307 kb on + strand at 2462.308 kb on - strand at 2462.308 kb on - strand at 2462.308 kb on - strand at 2462.310 kb on + strand at 2462.361 kb on + strand, within ygaM at 2462.361 kb on + strand, within ygaM at 2462.365 kb on + strand, within ygaM at 2462.461 kb on - strand, within ygaM at 2462.509 kb on - strand, within ygaM at 2462.558 kb on - strand, within ygaM at 2462.558 kb on - strand, within ygaM at 2462.652 kb on + strand at 2462.652 kb on + strand at 2462.653 kb on - strand at 2462.653 kb on - strand at 2462.689 kb on + strand at 2462.766 kb on + strand at 2462.767 kb on - strand at 2462.789 kb on + strand at 2462.803 kb on + strand at 2462.804 kb on - strand at 2462.804 kb on - strand at 2462.804 kb on - strand at 2462.807 kb on + strand at 2462.808 kb on - strand at 2462.808 kb on - strand at 2462.808 kb on - strand at 2462.808 kb on - strand at 2462.808 kb on - strand at 2462.854 kb on + strand, within ygaC at 2462.854 kb on + strand, within ygaC at 2462.883 kb on - strand, within ygaC at 2462.906 kb on + strand, within ygaC at 2462.912 kb on + strand, within ygaC at 2462.912 kb on + strand, within ygaC at 2462.912 kb on + strand, within ygaC at 2462.913 kb on - strand, within ygaC at 2462.913 kb on - strand, within ygaC at 2462.967 kb on + strand, within ygaC at 2463.044 kb on + strand, within ygaC at 2463.048 kb on + strand, within ygaC at 2463.049 kb on - strand, within ygaC at 2463.071 kb on + strand, within ygaC at 2463.072 kb on - strand, within ygaC at 2463.072 kb on - strand, within ygaC at 2463.076 kb on + strand, within ygaC at 2463.195 kb on - strand at 2463.247 kb on - strand, within alaE at 2463.253 kb on - strand, within alaE at 2463.304 kb on + strand, within alaE at 2463.348 kb on + strand, within alaE at 2463.348 kb on + strand, within alaE at 2463.349 kb on - strand, within alaE at 2463.349 kb on - strand, within alaE at 2463.450 kb on + strand, within alaE at 2463.451 kb on - strand, within alaE at 2463.454 kb on + strand, within alaE at 2463.455 kb on - strand, within alaE at 2463.474 kb on - strand, within alaE at 2463.474 kb on - strand, within alaE at 2463.474 kb on - strand, within alaE at 2463.474 kb on - strand, within alaE at 2463.474 kb on - strand, within alaE at 2463.508 kb on - strand, within alaE at 2463.551 kb on - strand, within alaE at 2463.574 kb on - strand, within alaE at 2463.592 kb on + strand at 2463.592 kb on + strand at 2463.592 kb on + strand at 2463.592 kb on + strand at 2463.593 kb on - strand at 2463.637 kb on + strand at 2463.637 kb on + strand at 2463.651 kb on - strand at 2463.659 kb on - strand at 2463.721 kb on + strand at 2463.721 kb on + strand at 2463.721 kb on + strand at 2463.721 kb on + strand at 2463.721 kb on + strand at 2463.722 kb on - strand at 2463.722 kb on - strand at 2463.722 kb on - strand at 2463.722 kb on - strand at 2463.812 kb on - strand at 2463.911 kb on + strand at 2463.939 kb on + strand at 2463.939 kb on + strand at 2463.939 kb on + strand at 2463.939 kb on + strand at 2463.939 kb on + strand at 2463.939 kb on + strand at 2463.940 kb on - strand at 2463.940 kb on - strand at 2463.986 kb on - strand at 2463.986 kb on - strand at 2464.122 kb on - strand at 2464.141 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas67 remove 2,461,817 + +1.3 2,461,817 + +0.2 2,461,818 - +1.3 2,461,899 - nrdH NOLOHH_12680 0.28 -0.4 2,461,982 + nrdH NOLOHH_12680 0.62 -2.0 2,461,983 - nrdH NOLOHH_12680 0.62 +1.1 2,462,186 + -0.4 2,462,186 + +0.3 2,462,186 + +3.3 2,462,187 - -2.5 2,462,304 - +0.9 2,462,307 + -0.7 2,462,308 - -0.8 2,462,308 - +0.5 2,462,308 - +0.4 2,462,310 + +0.6 2,462,361 + ygaM NOLOHH_12685 0.12 +0.3 2,462,361 + ygaM NOLOHH_12685 0.12 -0.7 2,462,365 + ygaM NOLOHH_12685 0.13 -0.7 2,462,461 - ygaM NOLOHH_12685 0.42 -1.7 2,462,509 - ygaM NOLOHH_12685 0.56 +0.5 2,462,558 - ygaM NOLOHH_12685 0.71 -0.1 2,462,558 - ygaM NOLOHH_12685 0.71 +1.3 2,462,652 + +1.9 2,462,652 + -0.9 2,462,653 - +1.3 2,462,653 - -0.3 2,462,689 + -0.7 2,462,766 + -0.9 2,462,767 - -1.1 2,462,789 + +1.3 2,462,803 + +0.8 2,462,804 - -0.9 2,462,804 - +0.3 2,462,804 - -0.0 2,462,807 + -0.8 2,462,808 - -0.5 2,462,808 - -1.2 2,462,808 - +2.5 2,462,808 - -0.8 2,462,808 - -0.0 2,462,854 + ygaC NOLOHH_12690 0.14 +1.7 2,462,854 + ygaC NOLOHH_12690 0.14 -0.8 2,462,883 - ygaC NOLOHH_12690 0.23 -0.1 2,462,906 + ygaC NOLOHH_12690 0.30 +0.6 2,462,912 + ygaC NOLOHH_12690 0.31 +0.6 2,462,912 + ygaC NOLOHH_12690 0.31 +1.1 2,462,912 + ygaC NOLOHH_12690 0.31 +0.5 2,462,913 - ygaC NOLOHH_12690 0.32 -0.2 2,462,913 - ygaC NOLOHH_12690 0.32 -2.2 2,462,967 + ygaC NOLOHH_12690 0.47 -0.4 2,463,044 + ygaC NOLOHH_12690 0.70 -0.7 2,463,048 + ygaC NOLOHH_12690 0.71 -0.7 2,463,049 - ygaC NOLOHH_12690 0.71 +0.3 2,463,071 + ygaC NOLOHH_12690 0.77 +1.1 2,463,072 - ygaC NOLOHH_12690 0.78 -1.2 2,463,072 - ygaC NOLOHH_12690 0.78 +2.3 2,463,076 + ygaC NOLOHH_12690 0.79 +0.1 2,463,195 - -1.0 2,463,247 - alaE NOLOHH_12695 0.14 +1.1 2,463,253 - alaE NOLOHH_12695 0.15 +0.3 2,463,304 + alaE NOLOHH_12695 0.26 -0.2 2,463,348 + alaE NOLOHH_12695 0.36 -2.0 2,463,348 + alaE NOLOHH_12695 0.36 -0.2 2,463,349 - alaE NOLOHH_12695 0.36 +0.2 2,463,349 - alaE NOLOHH_12695 0.36 -0.7 2,463,450 + alaE NOLOHH_12695 0.59 +0.6 2,463,451 - alaE NOLOHH_12695 0.59 -0.0 2,463,454 + alaE NOLOHH_12695 0.60 +0.3 2,463,455 - alaE NOLOHH_12695 0.60 +1.6 2,463,474 - alaE NOLOHH_12695 0.64 -0.7 2,463,474 - alaE NOLOHH_12695 0.64 -0.3 2,463,474 - alaE NOLOHH_12695 0.64 -0.8 2,463,474 - alaE NOLOHH_12695 0.64 +0.8 2,463,474 - alaE NOLOHH_12695 0.64 -0.5 2,463,508 - alaE NOLOHH_12695 0.72 +0.9 2,463,551 - alaE NOLOHH_12695 0.81 +0.8 2,463,574 - alaE NOLOHH_12695 0.86 +0.1 2,463,592 + -1.4 2,463,592 + -0.1 2,463,592 + +0.3 2,463,592 + -2.0 2,463,593 - +0.0 2,463,637 + +0.6 2,463,637 + +0.1 2,463,651 - +0.3 2,463,659 - +1.7 2,463,721 + -0.1 2,463,721 + -0.1 2,463,721 + +0.6 2,463,721 + -0.3 2,463,721 + +0.6 2,463,722 - +0.9 2,463,722 - -0.2 2,463,722 - +1.3 2,463,722 - -0.9 2,463,812 - -0.3 2,463,911 + +1.3 2,463,939 + -0.3 2,463,939 + +0.5 2,463,939 + -0.2 2,463,939 + +1.3 2,463,939 + -0.2 2,463,939 + +0.9 2,463,940 - -0.7 2,463,940 - +0.3 2,463,986 - -0.5 2,463,986 - +0.6 2,464,122 - -0.3 2,464,141 - +1.9
Or see this region's nucleotide sequence