Experiment: Bas67
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fusA and tufA_E379K are separated by 70 nucleotides tufA_E379K and bfd are separated by 182 nucleotides bfd and bfr are separated by 72 nucleotides bfr and NOLOHH_09150 overlap by 4 nucleotides
NOLOHH_09130: fusA - elongation factor G, at 1,774,310 to 1,776,424
fusA
NOLOHH_09135: tufA_E379K - translation elongation factor EF-Tu 1, at 1,776,495 to 1,777,679
_E379K
NOLOHH_09140: bfd - bacterioferritin-associated ferredoxin, at 1,777,862 to 1,778,056
bfd
NOLOHH_09145: bfr - bacterioferritin, at 1,778,129 to 1,778,605
bfr
NOLOHH_09150: NOLOHH_09150 - prepilin peptidase, at 1,778,602 to 1,779,069
_09150
Position (kb)
1776
1777
1778 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1776.477 kb on - strand at 1776.478 kb on + strand at 1776.479 kb on - strand at 1776.481 kb on + strand at 1776.488 kb on + strand at 1776.488 kb on + strand at 1776.489 kb on - strand at 1777.688 kb on + strand at 1777.692 kb on + strand at 1777.765 kb on - strand at 1777.783 kb on - strand at 1777.809 kb on + strand at 1777.865 kb on + strand at 1777.865 kb on + strand at 1777.865 kb on + strand at 1777.865 kb on + strand at 1777.866 kb on - strand at 1777.866 kb on - strand at 1777.866 kb on - strand at 1777.879 kb on + strand at 1777.879 kb on + strand at 1777.880 kb on - strand at 1777.887 kb on + strand, within bfd at 1777.887 kb on + strand, within bfd at 1777.888 kb on - strand, within bfd at 1777.957 kb on + strand, within bfd at 1777.981 kb on + strand, within bfd at 1778.095 kb on + strand at 1778.102 kb on + strand at 1778.102 kb on + strand at 1778.103 kb on - strand at 1778.103 kb on - strand at 1778.123 kb on + strand at 1778.125 kb on + strand at 1778.125 kb on + strand at 1778.126 kb on - strand at 1778.126 kb on - strand at 1778.156 kb on + strand at 1778.156 kb on + strand at 1778.164 kb on + strand at 1778.164 kb on + strand at 1778.165 kb on - strand at 1778.201 kb on + strand, within bfr at 1778.202 kb on - strand, within bfr at 1778.261 kb on + strand, within bfr at 1778.296 kb on + strand, within bfr at 1778.296 kb on + strand, within bfr at 1778.296 kb on + strand, within bfr at 1778.300 kb on + strand, within bfr at 1778.300 kb on + strand, within bfr at 1778.300 kb on + strand, within bfr at 1778.301 kb on - strand, within bfr at 1778.301 kb on - strand, within bfr at 1778.301 kb on - strand, within bfr at 1778.301 kb on - strand, within bfr at 1778.302 kb on + strand, within bfr at 1778.312 kb on - strand, within bfr at 1778.312 kb on - strand, within bfr at 1778.340 kb on + strand, within bfr at 1778.340 kb on + strand, within bfr at 1778.468 kb on + strand, within bfr at 1778.468 kb on + strand, within bfr at 1778.468 kb on + strand, within bfr at 1778.468 kb on + strand, within bfr at 1778.468 kb on + strand, within bfr at 1778.469 kb on - strand, within bfr at 1778.469 kb on - strand, within bfr at 1778.469 kb on - strand, within bfr at 1778.469 kb on - strand, within bfr at 1778.469 kb on - strand, within bfr at 1778.483 kb on - strand, within bfr at 1778.589 kb on + strand at 1778.590 kb on - strand at 1778.597 kb on - strand at 1778.614 kb on + strand at 1778.632 kb on + strand at 1778.633 kb on - strand at 1778.633 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas67 remove 1,776,477 - +0.5 1,776,478 + -1.5 1,776,479 - +0.5 1,776,481 + -1.1 1,776,488 + -1.5 1,776,488 + +0.5 1,776,489 - -0.5 1,777,688 + -0.7 1,777,692 + +1.1 1,777,765 - -1.1 1,777,783 - -0.1 1,777,809 + +0.5 1,777,865 + +1.6 1,777,865 + +0.2 1,777,865 + +1.2 1,777,865 + +0.1 1,777,866 - -2.5 1,777,866 - +0.3 1,777,866 - -1.0 1,777,879 + +0.1 1,777,879 + -1.1 1,777,880 - -0.5 1,777,887 + bfd NOLOHH_09140 0.13 +0.5 1,777,887 + bfd NOLOHH_09140 0.13 -1.1 1,777,888 - bfd NOLOHH_09140 0.13 -1.0 1,777,957 + bfd NOLOHH_09140 0.49 -0.1 1,777,981 + bfd NOLOHH_09140 0.61 -0.1 1,778,095 + +0.9 1,778,102 + +0.5 1,778,102 + -0.7 1,778,103 - -0.7 1,778,103 - -0.5 1,778,123 + -3.1 1,778,125 + +0.6 1,778,125 + +0.5 1,778,126 - +0.2 1,778,126 - +0.5 1,778,156 + -0.7 1,778,156 + +0.9 1,778,164 + -0.7 1,778,164 + -0.5 1,778,165 - -0.1 1,778,201 + bfr NOLOHH_09145 0.15 -0.8 1,778,202 - bfr NOLOHH_09145 0.15 -0.8 1,778,261 + bfr NOLOHH_09145 0.28 +0.8 1,778,296 + bfr NOLOHH_09145 0.35 +0.5 1,778,296 + bfr NOLOHH_09145 0.35 +0.1 1,778,296 + bfr NOLOHH_09145 0.35 -0.7 1,778,300 + bfr NOLOHH_09145 0.36 -0.6 1,778,300 + bfr NOLOHH_09145 0.36 -0.5 1,778,300 + bfr NOLOHH_09145 0.36 -1.2 1,778,301 - bfr NOLOHH_09145 0.36 -0.8 1,778,301 - bfr NOLOHH_09145 0.36 -1.5 1,778,301 - bfr NOLOHH_09145 0.36 +0.9 1,778,301 - bfr NOLOHH_09145 0.36 +0.2 1,778,302 + bfr NOLOHH_09145 0.36 +0.8 1,778,312 - bfr NOLOHH_09145 0.38 +0.2 1,778,312 - bfr NOLOHH_09145 0.38 -1.1 1,778,340 + bfr NOLOHH_09145 0.44 -0.4 1,778,340 + bfr NOLOHH_09145 0.44 +0.8 1,778,468 + bfr NOLOHH_09145 0.71 -0.5 1,778,468 + bfr NOLOHH_09145 0.71 +1.3 1,778,468 + bfr NOLOHH_09145 0.71 +0.2 1,778,468 + bfr NOLOHH_09145 0.71 -0.9 1,778,468 + bfr NOLOHH_09145 0.71 +0.2 1,778,469 - bfr NOLOHH_09145 0.71 -0.6 1,778,469 - bfr NOLOHH_09145 0.71 -0.8 1,778,469 - bfr NOLOHH_09145 0.71 -0.3 1,778,469 - bfr NOLOHH_09145 0.71 +0.3 1,778,469 - bfr NOLOHH_09145 0.71 +1.2 1,778,483 - bfr NOLOHH_09145 0.74 +2.6 1,778,589 + +1.1 1,778,590 - +0.5 1,778,597 - +0.8 1,778,614 + -0.5 1,778,632 + +0.5 1,778,633 - -0.5 1,778,633 - -1.5
Or see this region's nucleotide sequence