Strain Fitness in Escherichia coli ECOR27 around NOLOHH_03185

Experiment: Bas67

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntpdxA and rsmA overlap by 4 nucleotidesrsmA and apaG are separated by 2 nucleotidesapaG and apaH are separated by 6 nucleotidesapaH and NOLOHH_03195 are separated by 77 nucleotides NOLOHH_03175: pdxA - 4-hydroxythreonine-4-phosphate dehydrogenase PdxA, at 554,307 to 555,296 pdxA NOLOHH_03180: rsmA - 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase RsmA, at 555,293 to 556,114 rsmA NOLOHH_03185: apaG - Co2+/Mg2+ efflux protein ApaG, at 556,117 to 556,494 apaG NOLOHH_03190: apaH - bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH, at 556,501 to 557,343 apaH NOLOHH_03195: NOLOHH_03195 - Dihydrofolate reductase, at 557,421 to 558,011 _03195 Position (kb) 556 557Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 555.129 kb on + strand, within pdxAat 555.129 kb on + strand, within pdxAat 555.677 kb on + strand, within rsmAat 555.788 kb on + strand, within rsmAat 555.970 kb on + strand, within rsmAat 556.079 kb on + strandat 556.080 kb on - strandat 556.080 kb on - strandat 556.112 kb on + strandat 556.140 kb on + strandat 556.140 kb on + strandat 556.186 kb on - strand, within apaGat 556.195 kb on + strand, within apaGat 556.207 kb on + strand, within apaGat 556.207 kb on + strand, within apaGat 556.208 kb on - strand, within apaGat 556.208 kb on - strand, within apaGat 556.208 kb on - strand, within apaGat 556.209 kb on + strand, within apaGat 556.210 kb on - strand, within apaGat 556.335 kb on + strand, within apaGat 556.361 kb on - strand, within apaGat 556.361 kb on - strand, within apaGat 556.501 kb on - strandat 556.510 kb on + strandat 556.511 kb on - strandat 556.511 kb on - strandat 556.563 kb on + strandat 556.563 kb on + strandat 556.564 kb on - strandat 556.591 kb on - strand, within apaHat 556.651 kb on + strand, within apaHat 556.676 kb on + strand, within apaHat 556.676 kb on + strand, within apaHat 556.677 kb on - strand, within apaHat 556.677 kb on - strand, within apaHat 556.886 kb on + strand, within apaHat 556.916 kb on + strand, within apaHat 556.962 kb on + strand, within apaHat 556.963 kb on - strand, within apaHat 556.963 kb on - strand, within apaHat 556.966 kb on + strand, within apaHat 556.983 kb on + strand, within apaHat 556.983 kb on + strand, within apaHat 557.079 kb on + strand, within apaHat 557.084 kb on - strand, within apaHat 557.205 kb on + strand, within apaHat 557.217 kb on + strand, within apaHat 557.218 kb on - strand, within apaHat 557.218 kb on - strand, within apaHat 557.218 kb on - strand, within apaHat 557.218 kb on - strand, within apaHat 557.219 kb on + strand, within apaHat 557.219 kb on + strand, within apaHat 557.219 kb on + strand, within apaHat 557.219 kb on + strand, within apaHat 557.220 kb on - strand, within apaHat 557.220 kb on - strand, within apaHat 557.221 kb on + strand, within apaHat 557.221 kb on + strand, within apaHat 557.221 kb on + strand, within apaHat 557.222 kb on - strand, within apaHat 557.222 kb on - strand, within apaHat 557.222 kb on - strand, within apaHat 557.253 kb on + strand, within apaHat 557.254 kb on - strand, within apaHat 557.254 kb on - strand, within apaHat 557.287 kb on + strandat 557.288 kb on - strandat 557.342 kb on - strandat 557.357 kb on + strandat 557.357 kb on + strandat 557.357 kb on + strandat 557.358 kb on - strandat 557.358 kb on - strandat 557.358 kb on - strandat 557.358 kb on - strandat 557.399 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas67
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555,129 + pdxA NOLOHH_03175 0.83 +0.1
555,129 + pdxA NOLOHH_03175 0.83 +3.0
555,677 + rsmA NOLOHH_03180 0.47 +0.4
555,788 + rsmA NOLOHH_03180 0.60 -0.6
555,970 + rsmA NOLOHH_03180 0.82 +0.4
556,079 + +1.2
556,080 - -0.7
556,080 - +1.4
556,112 + -1.2
556,140 + -0.7
556,140 + -0.6
556,186 - apaG NOLOHH_03185 0.18 -0.9
556,195 + apaG NOLOHH_03185 0.21 -3.6
556,207 + apaG NOLOHH_03185 0.24 -0.6
556,207 + apaG NOLOHH_03185 0.24 +0.2
556,208 - apaG NOLOHH_03185 0.24 -0.1
556,208 - apaG NOLOHH_03185 0.24 -1.4
556,208 - apaG NOLOHH_03185 0.24 -1.9
556,209 + apaG NOLOHH_03185 0.24 +0.2
556,210 - apaG NOLOHH_03185 0.25 -0.6
556,335 + apaG NOLOHH_03185 0.58 -1.6
556,361 - apaG NOLOHH_03185 0.65 +0.4
556,361 - apaG NOLOHH_03185 0.65 +1.0
556,501 - +2.0
556,510 + +0.4
556,511 - +1.4
556,511 - +0.4
556,563 + -0.6
556,563 + +1.4
556,564 - +0.4
556,591 - apaH NOLOHH_03190 0.11 +0.4
556,651 + apaH NOLOHH_03190 0.18 -1.9
556,676 + apaH NOLOHH_03190 0.21 -0.6
556,676 + apaH NOLOHH_03190 0.21 +0.4
556,677 - apaH NOLOHH_03190 0.21 -1.6
556,677 - apaH NOLOHH_03190 0.21 -1.9
556,886 + apaH NOLOHH_03190 0.46 -1.2
556,916 + apaH NOLOHH_03190 0.49 +1.4
556,962 + apaH NOLOHH_03190 0.55 +0.4
556,963 - apaH NOLOHH_03190 0.55 -1.2
556,963 - apaH NOLOHH_03190 0.55 -1.2
556,966 + apaH NOLOHH_03190 0.55 +1.4
556,983 + apaH NOLOHH_03190 0.57 -0.2
556,983 + apaH NOLOHH_03190 0.57 -0.6
557,079 + apaH NOLOHH_03190 0.69 +0.4
557,084 - apaH NOLOHH_03190 0.69 +0.4
557,205 + apaH NOLOHH_03190 0.84 -2.6
557,217 + apaH NOLOHH_03190 0.85 +1.0
557,218 - apaH NOLOHH_03190 0.85 -0.6
557,218 - apaH NOLOHH_03190 0.85 +0.4
557,218 - apaH NOLOHH_03190 0.85 -1.6
557,218 - apaH NOLOHH_03190 0.85 +0.4
557,219 + apaH NOLOHH_03190 0.85 -1.2
557,219 + apaH NOLOHH_03190 0.85 -1.7
557,219 + apaH NOLOHH_03190 0.85 -0.2
557,219 + apaH NOLOHH_03190 0.85 -0.6
557,220 - apaH NOLOHH_03190 0.85 +0.4
557,220 - apaH NOLOHH_03190 0.85 +0.4
557,221 + apaH NOLOHH_03190 0.85 -2.6
557,221 + apaH NOLOHH_03190 0.85 -0.6
557,221 + apaH NOLOHH_03190 0.85 +0.4
557,222 - apaH NOLOHH_03190 0.86 +0.4
557,222 - apaH NOLOHH_03190 0.86 -1.6
557,222 - apaH NOLOHH_03190 0.86 -0.6
557,253 + apaH NOLOHH_03190 0.89 -1.6
557,254 - apaH NOLOHH_03190 0.89 +2.0
557,254 - apaH NOLOHH_03190 0.89 -1.2
557,287 + -0.2
557,288 - +0.6
557,342 - +2.0
557,357 + +0.2
557,357 + -0.7
557,357 + -0.3
557,358 - -0.4
557,358 - -0.8
557,358 - +0.8
557,358 - -0.4
557,399 + +1.0

Or see this region's nucleotide sequence