Experiment: Bas67
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt guaC and yacM are separated by 155 nucleotides yacM and coaE are separated by 24 nucleotides coaE and zapD overlap by 1 nucleotides zapD and yacG are separated by 9 nucleotides yacG and mutT are separated by 99 nucleotides mutT and secA are separated by 59 nucleotides
NOLOHH_02895: guaC - GMP reductase, at 494,859 to 495,902
guaC
NOLOHH_02900: yacM - Protein YacM, at 496,058 to 496,102
yacM
NOLOHH_02905: coaE - dephospho-CoA kinase, at 496,127 to 496,747
coaE
NOLOHH_02910: zapD - cell division protein ZapD, at 496,747 to 497,490
zapD
NOLOHH_02915: yacG - DNA gyrase inhibitor YacG, at 497,500 to 497,697
yacG
NOLOHH_02925: mutT - 8-oxo-dGTP diphosphatase MutT, at 497,797 to 498,186
mutT
NOLOHH_02935: secA - preprotein translocase subunit SecA, at 498,246 to 500,951
secA
Position (kb) 496
497
498 Strain fitness (log2 ratio) -2
-1
0
1
2 at 495.834 kb on - strand at 495.896 kb on + strand at 495.927 kb on + strand at 495.927 kb on + strand at 495.929 kb on + strand at 495.929 kb on + strand at 495.935 kb on + strand at 495.964 kb on - strand at 496.008 kb on + strand at 496.013 kb on + strand at 496.137 kb on + strand at 496.137 kb on + strand at 496.137 kb on + strand at 496.137 kb on + strand at 496.137 kb on + strand at 496.138 kb on - strand at 496.138 kb on - strand at 496.138 kb on - strand at 496.745 kb on + strand at 496.746 kb on - strand at 496.778 kb on + strand at 496.779 kb on - strand at 496.794 kb on + strand at 496.794 kb on + strand at 496.794 kb on + strand at 496.794 kb on + strand at 496.795 kb on - strand at 496.895 kb on + strand, within zapD at 496.895 kb on + strand, within zapD at 496.895 kb on + strand, within zapD at 496.896 kb on - strand, within zapD at 496.962 kb on + strand, within zapD at 496.962 kb on + strand, within zapD at 496.962 kb on + strand, within zapD at 496.963 kb on - strand, within zapD at 497.018 kb on + strand, within zapD at 497.048 kb on + strand, within zapD at 497.051 kb on + strand, within zapD at 497.051 kb on + strand, within zapD at 497.052 kb on - strand, within zapD at 497.153 kb on + strand, within zapD at 497.153 kb on + strand, within zapD at 497.157 kb on + strand, within zapD at 497.158 kb on - strand, within zapD at 497.158 kb on - strand, within zapD at 497.158 kb on - strand, within zapD at 497.181 kb on - strand, within zapD at 497.250 kb on + strand, within zapD at 497.250 kb on + strand, within zapD at 497.250 kb on + strand, within zapD at 497.250 kb on + strand, within zapD at 497.250 kb on + strand, within zapD at 497.250 kb on + strand, within zapD at 497.250 kb on + strand, within zapD at 497.250 kb on + strand, within zapD at 497.251 kb on - strand, within zapD at 497.293 kb on - strand, within zapD at 497.400 kb on + strand, within zapD at 497.400 kb on + strand, within zapD at 497.400 kb on + strand, within zapD at 497.400 kb on + strand, within zapD at 497.401 kb on - strand, within zapD at 497.401 kb on - strand, within zapD at 497.401 kb on - strand, within zapD at 497.401 kb on - strand, within zapD at 497.401 kb on - strand, within zapD at 497.401 kb on - strand, within zapD at 497.401 kb on - strand, within zapD at 497.401 kb on - strand, within zapD at 497.511 kb on + strand at 497.511 kb on + strand at 497.511 kb on + strand at 497.511 kb on + strand at 497.511 kb on + strand at 497.512 kb on - strand at 497.512 kb on - strand at 497.947 kb on + strand, within mutT at 497.948 kb on - strand, within mutT at 497.948 kb on - strand, within mutT at 497.948 kb on - strand, within mutT at 497.948 kb on - strand, within mutT at 497.948 kb on - strand, within mutT at 497.948 kb on - strand, within mutT at 497.948 kb on - strand, within mutT at 497.948 kb on - strand, within mutT at 497.948 kb on - strand, within mutT at 497.948 kb on - strand, within mutT at 497.948 kb on - strand, within mutT at 497.948 kb on - strand, within mutT at 497.948 kb on - strand, within mutT at 497.948 kb on - strand, within mutT at 497.948 kb on - strand, within mutT at 497.950 kb on - strand, within mutT at 497.950 kb on - strand, within mutT at 497.950 kb on - strand, within mutT at 497.969 kb on + strand, within mutT at 498.007 kb on - strand, within mutT at 498.093 kb on + strand, within mutT at 498.127 kb on + strand, within mutT at 498.130 kb on - strand, within mutT at 498.156 kb on + strand at 498.159 kb on + strand at 498.160 kb on - strand at 498.160 kb on - strand at 498.247 kb on + strand at 498.275 kb on - strand at 498.309 kb on - strand at 498.455 kb on + strand at 498.456 kb on - strand at 498.456 kb on - strand at 498.456 kb on - strand at 498.456 kb on - strand at 498.456 kb on - strand at 498.460 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas67 remove 495,834 - -0.2 495,896 + +0.3 495,927 + -0.4 495,927 + +0.4 495,929 + -0.2 495,929 + -0.5 495,935 + +0.0 495,964 - +0.4 496,008 + -0.7 496,013 + -0.3 496,137 + -0.6 496,137 + +0.1 496,137 + -0.1 496,137 + -0.7 496,137 + -0.3 496,138 - -1.2 496,138 - -0.6 496,138 - -0.2 496,745 + +1.0 496,746 - +1.4 496,778 + -0.6 496,779 - -1.6 496,794 + +0.8 496,794 + +1.4 496,794 + -2.6 496,794 + -1.0 496,795 - -1.8 496,895 + zapD NOLOHH_02910 0.20 -0.9 496,895 + zapD NOLOHH_02910 0.20 +0.3 496,895 + zapD NOLOHH_02910 0.20 +0.3 496,896 - zapD NOLOHH_02910 0.20 -0.4 496,962 + zapD NOLOHH_02910 0.29 -0.6 496,962 + zapD NOLOHH_02910 0.29 +1.4 496,962 + zapD NOLOHH_02910 0.29 +2.7 496,963 - zapD NOLOHH_02910 0.29 +0.6 497,018 + zapD NOLOHH_02910 0.36 -0.8 497,048 + zapD NOLOHH_02910 0.40 +0.1 497,051 + zapD NOLOHH_02910 0.41 -0.4 497,051 + zapD NOLOHH_02910 0.41 -0.3 497,052 - zapD NOLOHH_02910 0.41 +1.2 497,153 + zapD NOLOHH_02910 0.55 -0.6 497,153 + zapD NOLOHH_02910 0.55 -1.0 497,157 + zapD NOLOHH_02910 0.55 +0.4 497,158 - zapD NOLOHH_02910 0.55 -0.6 497,158 - zapD NOLOHH_02910 0.55 +0.4 497,158 - zapD NOLOHH_02910 0.55 -0.2 497,181 - zapD NOLOHH_02910 0.58 +0.4 497,250 + zapD NOLOHH_02910 0.68 +0.1 497,250 + zapD NOLOHH_02910 0.68 +1.4 497,250 + zapD NOLOHH_02910 0.68 +0.2 497,250 + zapD NOLOHH_02910 0.68 -0.8 497,250 + zapD NOLOHH_02910 0.68 -0.1 497,250 + zapD NOLOHH_02910 0.68 -1.2 497,250 + zapD NOLOHH_02910 0.68 -0.2 497,250 + zapD NOLOHH_02910 0.68 +1.1 497,251 - zapD NOLOHH_02910 0.68 -1.3 497,293 - zapD NOLOHH_02910 0.73 +1.3 497,400 + zapD NOLOHH_02910 0.88 +2.4 497,400 + zapD NOLOHH_02910 0.88 +1.4 497,400 + zapD NOLOHH_02910 0.88 -0.7 497,400 + zapD NOLOHH_02910 0.88 -0.1 497,401 - zapD NOLOHH_02910 0.88 +1.4 497,401 - zapD NOLOHH_02910 0.88 +1.2 497,401 - zapD NOLOHH_02910 0.88 -0.8 497,401 - zapD NOLOHH_02910 0.88 +0.4 497,401 - zapD NOLOHH_02910 0.88 +0.9 497,401 - zapD NOLOHH_02910 0.88 +0.8 497,401 - zapD NOLOHH_02910 0.88 +0.8 497,401 - zapD NOLOHH_02910 0.88 -1.4 497,511 + -0.1 497,511 + -0.6 497,511 + +2.4 497,511 + +0.4 497,511 + -1.2 497,512 - +0.4 497,512 - +1.0 497,947 + mutT NOLOHH_02925 0.38 -0.2 497,948 - mutT NOLOHH_02925 0.39 -0.4 497,948 - mutT NOLOHH_02925 0.39 +0.2 497,948 - mutT NOLOHH_02925 0.39 +0.4 497,948 - mutT NOLOHH_02925 0.39 -1.6 497,948 - mutT NOLOHH_02925 0.39 +0.2 497,948 - mutT NOLOHH_02925 0.39 +0.5 497,948 - mutT NOLOHH_02925 0.39 +0.2 497,948 - mutT NOLOHH_02925 0.39 -1.1 497,948 - mutT NOLOHH_02925 0.39 +0.2 497,948 - mutT NOLOHH_02925 0.39 +1.1 497,948 - mutT NOLOHH_02925 0.39 +0.0 497,948 - mutT NOLOHH_02925 0.39 -0.0 497,948 - mutT NOLOHH_02925 0.39 -0.0 497,948 - mutT NOLOHH_02925 0.39 +0.4 497,948 - mutT NOLOHH_02925 0.39 -0.5 497,950 - mutT NOLOHH_02925 0.39 -0.9 497,950 - mutT NOLOHH_02925 0.39 +1.3 497,950 - mutT NOLOHH_02925 0.39 -2.2 497,969 + mutT NOLOHH_02925 0.44 +1.4 498,007 - mutT NOLOHH_02925 0.54 +0.4 498,093 + mutT NOLOHH_02925 0.76 +1.4 498,127 + mutT NOLOHH_02925 0.85 -0.6 498,130 - mutT NOLOHH_02925 0.85 +0.4 498,156 + +1.6 498,159 + -0.8 498,160 - -1.8 498,160 - -1.8 498,247 + +0.4 498,275 - -1.8 498,309 - +2.4 498,455 + -1.6 498,456 - -0.0 498,456 - -1.5 498,456 - -0.0 498,456 - -1.3 498,456 - -0.5 498,460 - +1.0
Or see this region's nucleotide sequence