Strain Fitness in Escherichia coli ECOR27 around NOLOHH_02450

Experiment: Bas67

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntarfB and yaeQ overlap by 4 nucleotidesyaeQ and yaeP are separated by 165 nucleotidesyaeP and rof overlap by 14 nucleotidesrof and tilS are separated by 48 nucleotides NOLOHH_02440: arfB - aminoacyl-tRNA hydrolase, at 395,134 to 395,556 arfB NOLOHH_02445: yaeQ - Uncharacterized protein YaeQ, at 395,553 to 396,098 yaeQ NOLOHH_02450: yaeP - YaeP family protein, at 396,264 to 396,464 yaeP NOLOHH_02455: rof - Rho-binding antiterminator, at 396,451 to 396,711 rof NOLOHH_02460: tilS - tRNA lysidine(34) synthetase TilS, at 396,760 to 398,058 tilS Position (kb) 396 397Strain fitness (log2 ratio) -2 -1 0 1at 395.264 kb on - strand, within arfBat 395.402 kb on + strand, within arfBat 395.403 kb on - strand, within arfBat 395.403 kb on - strand, within arfBat 395.403 kb on - strand, within arfBat 395.463 kb on + strand, within arfBat 395.467 kb on - strand, within arfBat 395.582 kb on + strandat 395.583 kb on - strandat 395.674 kb on - strand, within yaeQat 395.702 kb on - strand, within yaeQat 395.775 kb on - strand, within yaeQat 395.775 kb on - strand, within yaeQat 395.777 kb on + strand, within yaeQat 395.777 kb on + strand, within yaeQat 395.777 kb on + strand, within yaeQat 395.778 kb on - strand, within yaeQat 395.778 kb on - strand, within yaeQat 395.780 kb on - strand, within yaeQat 395.780 kb on - strand, within yaeQat 395.783 kb on + strand, within yaeQat 395.784 kb on - strand, within yaeQat 395.784 kb on - strand, within yaeQat 395.950 kb on + strand, within yaeQat 395.951 kb on - strand, within yaeQat 395.981 kb on + strand, within yaeQat 395.982 kb on - strand, within yaeQat 396.074 kb on + strandat 396.075 kb on - strandat 396.139 kb on + strandat 396.139 kb on + strandat 396.180 kb on + strandat 396.180 kb on + strandat 396.181 kb on - strandat 396.182 kb on + strandat 396.183 kb on - strandat 396.205 kb on + strandat 396.214 kb on - strandat 396.238 kb on - strandat 396.283 kb on + strandat 396.286 kb on + strand, within yaePat 396.322 kb on + strand, within yaePat 396.322 kb on + strand, within yaePat 396.323 kb on - strand, within yaePat 396.327 kb on + strand, within yaePat 396.420 kb on + strand, within yaePat 396.420 kb on + strand, within yaePat 396.421 kb on - strand, within yaePat 396.436 kb on + strand, within yaePat 396.450 kb on + strandat 396.450 kb on + strandat 396.450 kb on + strandat 396.451 kb on - strandat 396.465 kb on + strandat 396.469 kb on + strandat 396.469 kb on + strandat 396.469 kb on + strandat 396.469 kb on + strandat 396.469 kb on + strandat 396.493 kb on + strand, within rofat 396.493 kb on + strand, within rofat 396.527 kb on + strand, within rofat 396.608 kb on - strand, within rofat 396.645 kb on - strand, within rofat 396.645 kb on - strand, within rofat 396.661 kb on - strand, within rofat 396.761 kb on + strandat 396.761 kb on + strandat 396.766 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas67
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395,264 - arfB NOLOHH_02440 0.31 -1.0
395,402 + arfB NOLOHH_02440 0.63 -0.5
395,403 - arfB NOLOHH_02440 0.64 -0.4
395,403 - arfB NOLOHH_02440 0.64 -1.4
395,403 - arfB NOLOHH_02440 0.64 +1.4
395,463 + arfB NOLOHH_02440 0.78 +0.3
395,467 - arfB NOLOHH_02440 0.79 +0.9
395,582 + -0.7
395,583 - -0.6
395,674 - yaeQ NOLOHH_02445 0.22 -1.2
395,702 - yaeQ NOLOHH_02445 0.27 +1.4
395,775 - yaeQ NOLOHH_02445 0.41 +0.2
395,775 - yaeQ NOLOHH_02445 0.41 +0.0
395,777 + yaeQ NOLOHH_02445 0.41 -0.6
395,777 + yaeQ NOLOHH_02445 0.41 +1.0
395,777 + yaeQ NOLOHH_02445 0.41 -1.2
395,778 - yaeQ NOLOHH_02445 0.41 -2.2
395,778 - yaeQ NOLOHH_02445 0.41 -0.2
395,780 - yaeQ NOLOHH_02445 0.42 -0.2
395,780 - yaeQ NOLOHH_02445 0.42 -0.6
395,783 + yaeQ NOLOHH_02445 0.42 -0.2
395,784 - yaeQ NOLOHH_02445 0.42 -0.6
395,784 - yaeQ NOLOHH_02445 0.42 -0.4
395,950 + yaeQ NOLOHH_02445 0.73 +1.4
395,951 - yaeQ NOLOHH_02445 0.73 -0.0
395,981 + yaeQ NOLOHH_02445 0.78 +0.5
395,982 - yaeQ NOLOHH_02445 0.79 -0.2
396,074 + -0.6
396,075 - -0.1
396,139 + +1.4
396,139 + +0.1
396,180 + +0.4
396,180 + -0.0
396,181 - +0.2
396,182 + -0.1
396,183 - -1.6
396,205 + -1.2
396,214 - +1.4
396,238 - +0.4
396,283 + -0.3
396,286 + yaeP NOLOHH_02450 0.11 +1.4
396,322 + yaeP NOLOHH_02450 0.29 -0.5
396,322 + yaeP NOLOHH_02450 0.29 +1.4
396,323 - yaeP NOLOHH_02450 0.29 -0.8
396,327 + yaeP NOLOHH_02450 0.31 +0.3
396,420 + yaeP NOLOHH_02450 0.78 +0.6
396,420 + yaeP NOLOHH_02450 0.78 -0.2
396,421 - yaeP NOLOHH_02450 0.78 +1.0
396,436 + yaeP NOLOHH_02450 0.86 -0.4
396,450 + +1.0
396,450 + -2.6
396,450 + -0.2
396,451 - -0.0
396,465 + -0.2
396,469 + +0.2
396,469 + +0.4
396,469 + -0.3
396,469 + -0.4
396,469 + -1.2
396,493 + rof NOLOHH_02455 0.16 +0.2
396,493 + rof NOLOHH_02455 0.16 +1.0
396,527 + rof NOLOHH_02455 0.29 -0.8
396,608 - rof NOLOHH_02455 0.60 +0.4
396,645 - rof NOLOHH_02455 0.74 +0.7
396,645 - rof NOLOHH_02455 0.74 +0.4
396,661 - rof NOLOHH_02455 0.80 +0.1
396,761 + +0.4
396,761 + -0.6
396,766 - +0.9

Or see this region's nucleotide sequence