Experiment: Bas67
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt metQ and rcsF are separated by 117 nucleotides rcsF and tsaA overlap by 4 nucleotides tsaA and proS are separated by 110 nucleotides
NOLOHH_02405: metQ - methionine ABC transporter substrate-binding lipoprotein MetQ, at 388,230 to 389,045
metQ
NOLOHH_02410: rcsF - Rcs stress response system protein RcsF, at 389,163 to 389,567
rcsF
NOLOHH_02415: tsaA - tRNA (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase TrmO, at 389,564 to 390,271
tsaA
NOLOHH_02420: proS - proline--tRNA ligase, at 390,382 to 392,100
proS
Position (kb) 389
390
391 Strain fitness (log2 ratio) -2
-1
0
1
2 at 388.650 kb on - strand, within metQ at 388.842 kb on + strand, within metQ at 388.842 kb on + strand, within metQ at 388.842 kb on + strand, within metQ at 388.842 kb on + strand, within metQ at 388.843 kb on - strand, within metQ at 388.895 kb on + strand, within metQ at 388.908 kb on + strand, within metQ at 388.912 kb on + strand, within metQ at 388.912 kb on + strand, within metQ at 388.913 kb on - strand, within metQ at 388.995 kb on + strand at 388.995 kb on + strand at 388.996 kb on - strand at 389.070 kb on + strand at 389.070 kb on + strand at 389.071 kb on - strand at 389.073 kb on + strand at 389.074 kb on - strand at 389.111 kb on + strand at 389.125 kb on + strand at 389.146 kb on + strand at 389.146 kb on + strand at 389.147 kb on - strand at 389.147 kb on - strand at 389.159 kb on + strand at 389.159 kb on + strand at 389.159 kb on + strand at 389.160 kb on - strand at 389.162 kb on + strand at 389.162 kb on + strand at 389.163 kb on - strand at 389.185 kb on + strand at 389.200 kb on + strand at 389.200 kb on + strand at 389.316 kb on + strand, within rcsF at 389.317 kb on - strand, within rcsF at 389.317 kb on - strand, within rcsF at 389.318 kb on + strand, within rcsF at 389.319 kb on - strand, within rcsF at 389.335 kb on + strand, within rcsF at 389.335 kb on + strand, within rcsF at 389.429 kb on + strand, within rcsF at 389.429 kb on + strand, within rcsF at 389.476 kb on + strand, within rcsF at 389.476 kb on + strand, within rcsF at 389.483 kb on + strand, within rcsF at 389.483 kb on + strand, within rcsF at 389.530 kb on + strand at 389.530 kb on + strand at 389.530 kb on + strand at 389.530 kb on + strand at 389.530 kb on + strand at 389.530 kb on + strand at 389.530 kb on + strand at 389.530 kb on + strand at 389.530 kb on + strand at 389.530 kb on + strand at 389.535 kb on - strand at 389.649 kb on + strand, within tsaA at 389.688 kb on - strand, within tsaA at 389.688 kb on - strand, within tsaA at 389.688 kb on - strand, within tsaA at 389.688 kb on - strand, within tsaA at 389.842 kb on + strand, within tsaA at 389.866 kb on + strand, within tsaA at 389.975 kb on + strand, within tsaA at 390.017 kb on + strand, within tsaA at 390.017 kb on + strand, within tsaA at 390.017 kb on + strand, within tsaA at 390.132 kb on + strand, within tsaA at 390.132 kb on + strand, within tsaA at 390.133 kb on - strand, within tsaA at 390.163 kb on + strand, within tsaA at 390.164 kb on - strand, within tsaA at 390.188 kb on + strand, within tsaA at 390.188 kb on + strand, within tsaA at 390.189 kb on - strand, within tsaA at 390.269 kb on + strand at 390.315 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas67 remove 388,650 - metQ NOLOHH_02405 0.51 +0.4 388,842 + metQ NOLOHH_02405 0.75 -0.1 388,842 + metQ NOLOHH_02405 0.75 +0.8 388,842 + metQ NOLOHH_02405 0.75 +0.2 388,842 + metQ NOLOHH_02405 0.75 -0.6 388,843 - metQ NOLOHH_02405 0.75 -0.1 388,895 + metQ NOLOHH_02405 0.81 +0.1 388,908 + metQ NOLOHH_02405 0.83 +1.2 388,912 + metQ NOLOHH_02405 0.84 -0.7 388,912 + metQ NOLOHH_02405 0.84 -0.1 388,913 - metQ NOLOHH_02405 0.84 -0.5 388,995 + +0.2 388,995 + +1.0 388,996 - -0.6 389,070 + -0.6 389,070 + -0.4 389,071 - +2.0 389,073 + -0.6 389,074 - -0.6 389,111 + +0.1 389,125 + +0.4 389,146 + -1.2 389,146 + +0.4 389,147 - -0.8 389,147 - +0.0 389,159 + -1.6 389,159 + -0.3 389,159 + -0.4 389,160 - -1.7 389,162 + +0.8 389,162 + -0.1 389,163 - -0.2 389,185 + +1.0 389,200 + -1.6 389,200 + +0.9 389,316 + rcsF NOLOHH_02410 0.38 -0.8 389,317 - rcsF NOLOHH_02410 0.38 -2.4 389,317 - rcsF NOLOHH_02410 0.38 -0.6 389,318 + rcsF NOLOHH_02410 0.38 +2.0 389,319 - rcsF NOLOHH_02410 0.39 +0.1 389,335 + rcsF NOLOHH_02410 0.42 -0.8 389,335 + rcsF NOLOHH_02410 0.42 -0.2 389,429 + rcsF NOLOHH_02410 0.66 +1.0 389,429 + rcsF NOLOHH_02410 0.66 +0.6 389,476 + rcsF NOLOHH_02410 0.77 +0.7 389,476 + rcsF NOLOHH_02410 0.77 -0.0 389,483 + rcsF NOLOHH_02410 0.79 -0.0 389,483 + rcsF NOLOHH_02410 0.79 +0.4 389,530 + -0.1 389,530 + -0.4 389,530 + +0.4 389,530 + +0.4 389,530 + -0.0 389,530 + -0.0 389,530 + +0.4 389,530 + -1.2 389,530 + -1.3 389,530 + -0.9 389,535 - +0.1 389,649 + tsaA NOLOHH_02415 0.12 -0.2 389,688 - tsaA NOLOHH_02415 0.18 -0.1 389,688 - tsaA NOLOHH_02415 0.18 -0.4 389,688 - tsaA NOLOHH_02415 0.18 -0.1 389,688 - tsaA NOLOHH_02415 0.18 -0.9 389,842 + tsaA NOLOHH_02415 0.39 -0.1 389,866 + tsaA NOLOHH_02415 0.43 +0.2 389,975 + tsaA NOLOHH_02415 0.58 -0.0 390,017 + tsaA NOLOHH_02415 0.64 -1.2 390,017 + tsaA NOLOHH_02415 0.64 -0.6 390,017 + tsaA NOLOHH_02415 0.64 +1.4 390,132 + tsaA NOLOHH_02415 0.80 +0.8 390,132 + tsaA NOLOHH_02415 0.80 +0.4 390,133 - tsaA NOLOHH_02415 0.80 -0.1 390,163 + tsaA NOLOHH_02415 0.85 +0.6 390,164 - tsaA NOLOHH_02415 0.85 +0.0 390,188 + tsaA NOLOHH_02415 0.88 +0.2 390,188 + tsaA NOLOHH_02415 0.88 +0.4 390,189 - tsaA NOLOHH_02415 0.88 +1.4 390,269 + +0.3 390,315 + +1.1
Or see this region's nucleotide sequence