Strain Fitness in Escherichia coli ECOR27 around NOLOHH_00645

Experiment: Bas67

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntNOLOHH_00620 and ylcG overlap by 4 nucleotidesylcG and rusA overlap by 1 nucleotidesrusA and ybcO overlap by 4 nucleotidesybcO and NOLOHH_00640 overlap by 8 nucleotidesNOLOHH_00640 and ybcN overlap by 1 nucleotidesybcN and NOLOHH_00650 are separated by 537 nucleotidesNOLOHH_00650 and NOLOHH_00655 are separated by 256 nucleotides NOLOHH_00620: NOLOHH_00620 - Orf33, orf151, orf56, orf96, rus, orf45, orf8, orf127, and nmpC genes, at 24,028 to 24,054 _00620 NOLOHH_00625: ylcG - Uncharacterized protein YlcG, at 24,051 to 24,188 ylcG NOLOHH_00630: rusA - crossover junction endodeoxyribonuclease RusA, at 24,188 to 24,550 rusA NOLOHH_00635: ybcO - Putative nuclease YbcO, at 24,547 to 24,837 ybcO NOLOHH_00640: NOLOHH_00640 - NINE Protein, at 24,830 to 25,000 _00640 NOLOHH_00645: ybcN - DNA base-flipping protein YbcN, at 25,000 to 25,455 ybcN NOLOHH_00650: NOLOHH_00650 - hypothetical protein, at 25,993 to 26,127 _00650 NOLOHH_00655: NOLOHH_00655 - DUF4145 domain-containing protein, at 26,384 to 27,136 _00655 Position (kb) 24 25 26Strain fitness (log2 ratio) -2 -1 0 1 2at 24.029 kb on + strandat 24.041 kb on - strand, within NOLOHH_00620at 24.054 kb on + strandat 24.131 kb on - strand, within ylcGat 24.131 kb on - strand, within ylcGat 24.132 kb on + strand, within ylcGat 24.132 kb on + strand, within ylcGat 24.132 kb on + strand, within ylcGat 24.132 kb on + strand, within ylcGat 24.133 kb on - strand, within ylcGat 24.133 kb on - strand, within ylcGat 24.133 kb on - strand, within ylcGat 24.177 kb on - strandat 24.579 kb on + strand, within ybcOat 24.623 kb on + strand, within ybcOat 24.774 kb on + strand, within ybcOat 24.775 kb on - strand, within ybcOat 24.775 kb on - strand, within ybcOat 24.775 kb on - strand, within ybcOat 24.778 kb on + strand, within ybcOat 24.778 kb on + strand, within ybcOat 24.794 kb on - strand, within ybcOat 24.872 kb on - strand, within NOLOHH_00640at 24.889 kb on + strand, within NOLOHH_00640at 24.889 kb on + strand, within NOLOHH_00640at 24.890 kb on - strand, within NOLOHH_00640at 24.890 kb on - strand, within NOLOHH_00640at 24.890 kb on - strand, within NOLOHH_00640at 24.900 kb on - strand, within NOLOHH_00640at 24.909 kb on - strand, within NOLOHH_00640at 24.935 kb on - strand, within NOLOHH_00640at 24.935 kb on - strand, within NOLOHH_00640at 24.940 kb on + strand, within NOLOHH_00640at 24.960 kb on + strand, within NOLOHH_00640at 24.961 kb on - strand, within NOLOHH_00640at 24.994 kb on + strandat 24.995 kb on - strandat 25.068 kb on + strand, within ybcNat 25.093 kb on - strand, within ybcNat 25.093 kb on - strand, within ybcNat 25.106 kb on + strand, within ybcNat 25.106 kb on + strand, within ybcNat 25.106 kb on + strand, within ybcNat 25.106 kb on + strand, within ybcNat 25.106 kb on + strand, within ybcNat 25.106 kb on + strand, within ybcNat 25.106 kb on + strand, within ybcNat 25.106 kb on + strand, within ybcNat 25.107 kb on - strand, within ybcNat 25.107 kb on - strand, within ybcNat 25.107 kb on - strand, within ybcNat 25.107 kb on - strand, within ybcNat 25.208 kb on + strand, within ybcNat 25.208 kb on + strand, within ybcNat 25.208 kb on + strand, within ybcNat 25.209 kb on - strand, within ybcNat 25.274 kb on + strand, within ybcNat 25.292 kb on + strand, within ybcNat 25.347 kb on + strand, within ybcNat 25.383 kb on - strand, within ybcNat 25.426 kb on + strandat 25.426 kb on + strandat 25.426 kb on + strandat 25.427 kb on - strandat 25.544 kb on - strandat 25.544 kb on - strandat 25.608 kb on + strandat 25.609 kb on - strandat 25.609 kb on - strandat 25.609 kb on - strandat 25.641 kb on + strandat 25.652 kb on - strandat 25.688 kb on - strandat 25.763 kb on - strandat 25.764 kb on + strandat 25.765 kb on - strandat 25.765 kb on - strandat 25.765 kb on - strandat 25.769 kb on - strandat 25.777 kb on + strandat 25.827 kb on + strandat 25.832 kb on - strandat 25.961 kb on - strandat 25.961 kb on - strandat 25.978 kb on + strandat 26.054 kb on + strand, within NOLOHH_00650at 26.095 kb on - strand, within NOLOHH_00650at 26.145 kb on - strandat 26.274 kb on - strandat 26.290 kb on + strandat 26.290 kb on + strandat 26.291 kb on - strandat 26.291 kb on - strandat 26.294 kb on + strandat 26.294 kb on + strandat 26.357 kb on + strandat 26.403 kb on + strandat 26.425 kb on + strandat 26.432 kb on - strandat 26.432 kb on - strandat 26.432 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas67
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24,029 + -1.2
24,041 - NOLOHH_00620 0.48 -1.9
24,054 + +0.1
24,131 - ylcG NOLOHH_00625 0.58 -0.6
24,131 - ylcG NOLOHH_00625 0.58 -0.7
24,132 + ylcG NOLOHH_00625 0.59 -1.2
24,132 + ylcG NOLOHH_00625 0.59 +1.4
24,132 + ylcG NOLOHH_00625 0.59 -1.9
24,132 + ylcG NOLOHH_00625 0.59 +0.4
24,133 - ylcG NOLOHH_00625 0.59 +1.6
24,133 - ylcG NOLOHH_00625 0.59 -1.2
24,133 - ylcG NOLOHH_00625 0.59 -0.7
24,177 - +0.8
24,579 + ybcO NOLOHH_00635 0.11 +0.4
24,623 + ybcO NOLOHH_00635 0.26 -1.4
24,774 + ybcO NOLOHH_00635 0.78 +1.4
24,775 - ybcO NOLOHH_00635 0.78 -1.6
24,775 - ybcO NOLOHH_00635 0.78 -0.5
24,775 - ybcO NOLOHH_00635 0.78 -0.6
24,778 + ybcO NOLOHH_00635 0.79 +0.0
24,778 + ybcO NOLOHH_00635 0.79 +0.4
24,794 - ybcO NOLOHH_00635 0.85 -1.2
24,872 - NOLOHH_00640 0.25 -0.8
24,889 + NOLOHH_00640 0.35 -1.0
24,889 + NOLOHH_00640 0.35 +0.1
24,890 - NOLOHH_00640 0.35 +0.6
24,890 - NOLOHH_00640 0.35 -0.4
24,890 - NOLOHH_00640 0.35 +1.4
24,900 - NOLOHH_00640 0.41 -0.7
24,909 - NOLOHH_00640 0.46 +0.9
24,935 - NOLOHH_00640 0.61 +0.1
24,935 - NOLOHH_00640 0.61 -1.7
24,940 + NOLOHH_00640 0.64 -0.9
24,960 + NOLOHH_00640 0.76 +2.3
24,961 - NOLOHH_00640 0.77 +0.1
24,994 + -1.6
24,995 - -1.2
25,068 + ybcN NOLOHH_00645 0.15 -0.7
25,093 - ybcN NOLOHH_00645 0.20 +0.1
25,093 - ybcN NOLOHH_00645 0.20 -0.3
25,106 + ybcN NOLOHH_00645 0.23 +0.7
25,106 + ybcN NOLOHH_00645 0.23 +1.4
25,106 + ybcN NOLOHH_00645 0.23 -0.6
25,106 + ybcN NOLOHH_00645 0.23 +0.3
25,106 + ybcN NOLOHH_00645 0.23 -0.3
25,106 + ybcN NOLOHH_00645 0.23 +0.1
25,106 + ybcN NOLOHH_00645 0.23 +0.6
25,106 + ybcN NOLOHH_00645 0.23 +1.8
25,107 - ybcN NOLOHH_00645 0.23 -0.5
25,107 - ybcN NOLOHH_00645 0.23 +0.2
25,107 - ybcN NOLOHH_00645 0.23 -0.2
25,107 - ybcN NOLOHH_00645 0.23 -0.9
25,208 + ybcN NOLOHH_00645 0.46 -1.6
25,208 + ybcN NOLOHH_00645 0.46 -0.2
25,208 + ybcN NOLOHH_00645 0.46 -0.4
25,209 - ybcN NOLOHH_00645 0.46 -1.0
25,274 + ybcN NOLOHH_00645 0.60 +0.4
25,292 + ybcN NOLOHH_00645 0.64 +0.1
25,347 + ybcN NOLOHH_00645 0.76 -0.2
25,383 - ybcN NOLOHH_00645 0.84 +2.4
25,426 + +1.4
25,426 + +0.4
25,426 + +1.2
25,427 - -1.9
25,544 - +0.1
25,544 - +0.6
25,608 + -2.4
25,609 - -0.6
25,609 - +0.0
25,609 - -0.4
25,641 + +0.1
25,652 - +0.0
25,688 - +0.9
25,763 - -0.3
25,764 + -0.6
25,765 - -0.1
25,765 - +0.2
25,765 - -0.2
25,769 - -1.6
25,777 + -0.2
25,827 + -1.2
25,832 - -2.4
25,961 - +0.4
25,961 - +0.8
25,978 + -0.2
26,054 + NOLOHH_00650 0.45 +0.4
26,095 - NOLOHH_00650 0.76 -1.2
26,145 - -1.2
26,274 - -0.7
26,290 + +0.0
26,290 + -0.4
26,291 - -0.4
26,291 - -1.9
26,294 + -1.3
26,294 + +0.1
26,357 + +0.7
26,403 + -0.9
26,425 + -0.3
26,432 - +1.0
26,432 - +0.8
26,432 - -0.7

Or see this region's nucleotide sequence