Experiment: Bas67
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt NOLOHH_00610 and quuD are separated by 189 nucleotides quuD and NOLOHH_00620 are separated by 62 nucleotides NOLOHH_00620 and ylcG overlap by 4 nucleotides ylcG and rusA overlap by 1 nucleotides rusA and ybcO overlap by 4 nucleotides ybcO and NOLOHH_00640 overlap by 8 nucleotides NOLOHH_00640 and ybcN overlap by 1 nucleotides
NOLOHH_00610: NOLOHH_00610 - Outer membrane porin protein LC, at 22,295 to 23,392
_00610
NOLOHH_00615: quuD - antitermination protein QuuD, at 23,582 to 23,965
quuD
NOLOHH_00620: NOLOHH_00620 - Orf33, orf151, orf56, orf96, rus, orf45, orf8, orf127, and nmpC genes, at 24,028 to 24,054
_00620
NOLOHH_00625: ylcG - Uncharacterized protein YlcG, at 24,051 to 24,188
ylcG
NOLOHH_00630: rusA - crossover junction endodeoxyribonuclease RusA, at 24,188 to 24,550
rusA
NOLOHH_00635: ybcO - Putative nuclease YbcO, at 24,547 to 24,837
ybcO
NOLOHH_00640: NOLOHH_00640 - NINE Protein, at 24,830 to 25,000
_00640
NOLOHH_00645: ybcN - DNA base-flipping protein YbcN, at 25,000 to 25,455
ybcN
Position (kb) 24
25 Strain fitness (log2 ratio) -2
-1
0
1
2 at 23.232 kb on + strand, within NOLOHH_00610 at 23.232 kb on + strand, within NOLOHH_00610 at 23.240 kb on + strand, within NOLOHH_00610 at 23.261 kb on + strand, within NOLOHH_00610 at 23.261 kb on + strand, within NOLOHH_00610 at 23.346 kb on + strand at 23.347 kb on - strand at 23.370 kb on + strand at 23.381 kb on + strand at 23.410 kb on + strand at 23.442 kb on + strand at 23.442 kb on + strand at 23.443 kb on - strand at 23.443 kb on - strand at 23.443 kb on - strand at 23.497 kb on + strand at 23.588 kb on - strand at 23.864 kb on + strand, within quuD at 23.972 kb on - strand at 24.029 kb on + strand at 24.041 kb on - strand, within NOLOHH_00620 at 24.054 kb on + strand at 24.131 kb on - strand, within ylcG at 24.131 kb on - strand, within ylcG at 24.132 kb on + strand, within ylcG at 24.132 kb on + strand, within ylcG at 24.132 kb on + strand, within ylcG at 24.132 kb on + strand, within ylcG at 24.133 kb on - strand, within ylcG at 24.133 kb on - strand, within ylcG at 24.133 kb on - strand, within ylcG at 24.177 kb on - strand at 24.579 kb on + strand, within ybcO at 24.623 kb on + strand, within ybcO at 24.774 kb on + strand, within ybcO at 24.775 kb on - strand, within ybcO at 24.775 kb on - strand, within ybcO at 24.775 kb on - strand, within ybcO at 24.778 kb on + strand, within ybcO at 24.778 kb on + strand, within ybcO at 24.794 kb on - strand, within ybcO at 24.872 kb on - strand, within NOLOHH_00640 at 24.889 kb on + strand, within NOLOHH_00640 at 24.889 kb on + strand, within NOLOHH_00640 at 24.890 kb on - strand, within NOLOHH_00640 at 24.890 kb on - strand, within NOLOHH_00640 at 24.890 kb on - strand, within NOLOHH_00640 at 24.900 kb on - strand, within NOLOHH_00640 at 24.909 kb on - strand, within NOLOHH_00640 at 24.935 kb on - strand, within NOLOHH_00640 at 24.935 kb on - strand, within NOLOHH_00640 at 24.940 kb on + strand, within NOLOHH_00640 at 24.960 kb on + strand, within NOLOHH_00640 at 24.961 kb on - strand, within NOLOHH_00640 at 24.994 kb on + strand at 24.995 kb on - strand at 25.068 kb on + strand, within ybcN at 25.093 kb on - strand, within ybcN at 25.093 kb on - strand, within ybcN at 25.106 kb on + strand, within ybcN at 25.106 kb on + strand, within ybcN at 25.106 kb on + strand, within ybcN at 25.106 kb on + strand, within ybcN at 25.106 kb on + strand, within ybcN at 25.106 kb on + strand, within ybcN at 25.106 kb on + strand, within ybcN at 25.106 kb on + strand, within ybcN at 25.107 kb on - strand, within ybcN at 25.107 kb on - strand, within ybcN at 25.107 kb on - strand, within ybcN at 25.107 kb on - strand, within ybcN at 25.208 kb on + strand, within ybcN at 25.208 kb on + strand, within ybcN at 25.208 kb on + strand, within ybcN at 25.209 kb on - strand, within ybcN at 25.274 kb on + strand, within ybcN at 25.292 kb on + strand, within ybcN at 25.347 kb on + strand, within ybcN at 25.383 kb on - strand, within ybcN at 25.426 kb on + strand at 25.426 kb on + strand at 25.426 kb on + strand at 25.427 kb on - strand at 25.544 kb on - strand at 25.544 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas67 remove 23,232 + NOLOHH_00610 0.85 -1.9 23,232 + NOLOHH_00610 0.85 +0.7 23,240 + NOLOHH_00610 0.86 +0.4 23,261 + NOLOHH_00610 0.88 +0.0 23,261 + NOLOHH_00610 0.88 +1.7 23,346 + -0.1 23,347 - +0.4 23,370 + -1.1 23,381 + +1.1 23,410 + +0.4 23,442 + -0.9 23,442 + +0.4 23,443 - -0.9 23,443 - +0.4 23,443 - -0.0 23,497 + +0.7 23,588 - -0.6 23,864 + quuD NOLOHH_00615 0.73 -0.2 23,972 - +1.6 24,029 + -1.2 24,041 - NOLOHH_00620 0.48 -1.9 24,054 + +0.1 24,131 - ylcG NOLOHH_00625 0.58 -0.6 24,131 - ylcG NOLOHH_00625 0.58 -0.7 24,132 + ylcG NOLOHH_00625 0.59 -1.2 24,132 + ylcG NOLOHH_00625 0.59 +1.4 24,132 + ylcG NOLOHH_00625 0.59 -1.9 24,132 + ylcG NOLOHH_00625 0.59 +0.4 24,133 - ylcG NOLOHH_00625 0.59 +1.6 24,133 - ylcG NOLOHH_00625 0.59 -1.2 24,133 - ylcG NOLOHH_00625 0.59 -0.7 24,177 - +0.8 24,579 + ybcO NOLOHH_00635 0.11 +0.4 24,623 + ybcO NOLOHH_00635 0.26 -1.4 24,774 + ybcO NOLOHH_00635 0.78 +1.4 24,775 - ybcO NOLOHH_00635 0.78 -1.6 24,775 - ybcO NOLOHH_00635 0.78 -0.5 24,775 - ybcO NOLOHH_00635 0.78 -0.6 24,778 + ybcO NOLOHH_00635 0.79 +0.0 24,778 + ybcO NOLOHH_00635 0.79 +0.4 24,794 - ybcO NOLOHH_00635 0.85 -1.2 24,872 - NOLOHH_00640 0.25 -0.8 24,889 + NOLOHH_00640 0.35 -1.0 24,889 + NOLOHH_00640 0.35 +0.1 24,890 - NOLOHH_00640 0.35 +0.6 24,890 - NOLOHH_00640 0.35 -0.4 24,890 - NOLOHH_00640 0.35 +1.4 24,900 - NOLOHH_00640 0.41 -0.7 24,909 - NOLOHH_00640 0.46 +0.9 24,935 - NOLOHH_00640 0.61 +0.1 24,935 - NOLOHH_00640 0.61 -1.7 24,940 + NOLOHH_00640 0.64 -0.9 24,960 + NOLOHH_00640 0.76 +2.3 24,961 - NOLOHH_00640 0.77 +0.1 24,994 + -1.6 24,995 - -1.2 25,068 + ybcN NOLOHH_00645 0.15 -0.7 25,093 - ybcN NOLOHH_00645 0.20 +0.1 25,093 - ybcN NOLOHH_00645 0.20 -0.3 25,106 + ybcN NOLOHH_00645 0.23 +0.7 25,106 + ybcN NOLOHH_00645 0.23 +1.4 25,106 + ybcN NOLOHH_00645 0.23 -0.6 25,106 + ybcN NOLOHH_00645 0.23 +0.3 25,106 + ybcN NOLOHH_00645 0.23 -0.3 25,106 + ybcN NOLOHH_00645 0.23 +0.1 25,106 + ybcN NOLOHH_00645 0.23 +0.6 25,106 + ybcN NOLOHH_00645 0.23 +1.8 25,107 - ybcN NOLOHH_00645 0.23 -0.5 25,107 - ybcN NOLOHH_00645 0.23 +0.2 25,107 - ybcN NOLOHH_00645 0.23 -0.2 25,107 - ybcN NOLOHH_00645 0.23 -0.9 25,208 + ybcN NOLOHH_00645 0.46 -1.6 25,208 + ybcN NOLOHH_00645 0.46 -0.2 25,208 + ybcN NOLOHH_00645 0.46 -0.4 25,209 - ybcN NOLOHH_00645 0.46 -1.0 25,274 + ybcN NOLOHH_00645 0.60 +0.4 25,292 + ybcN NOLOHH_00645 0.64 +0.1 25,347 + ybcN NOLOHH_00645 0.76 -0.2 25,383 - ybcN NOLOHH_00645 0.84 +2.4 25,426 + +1.4 25,426 + +0.4 25,426 + +1.2 25,427 - -1.9 25,544 - +0.1 25,544 - +0.6
Or see this region's nucleotide sequence