Strain Fitness in Escherichia coli ECOR27 around NOLOHH_24280

Experiment: Bas09

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntpsiA and psiB overlap by 4 nucleotidespsiB and yubM are separated by 54 nucleotidesyubM and yubL are separated by 65 nucleotidesyubL and ssb are separated by 57 nucleotides NOLOHH_24270: psiA - Protein PsiA, at 6,275 to 6,994 psiA NOLOHH_24275: psiB - conjugation system SOS inhibitor PsiB, at 6,991 to 7,425 psiB NOLOHH_24280: yubM - Uncharacterized protein YubM, at 7,480 to 9,444 yubM NOLOHH_24285: yubL - UPF0401 protein YubL, at 9,510 to 9,743 yubL NOLOHH_24290: ssb - single-stranded DNA-binding protein, at 9,801 to 10,328 ssb Position (kb) 7 8 9 10Strain fitness (log2 ratio) -2 -1 0 1 2at 6.613 kb on - strand, within psiAat 6.756 kb on + strand, within psiAat 6.756 kb on + strand, within psiAat 6.757 kb on - strand, within psiAat 6.876 kb on + strand, within psiAat 7.020 kb on + strandat 7.195 kb on + strand, within psiBat 7.196 kb on - strand, within psiBat 7.196 kb on - strand, within psiBat 7.373 kb on + strand, within psiBat 7.396 kb on + strandat 7.396 kb on + strandat 7.397 kb on - strandat 7.397 kb on - strandat 7.399 kb on + strandat 7.399 kb on + strandat 7.399 kb on + strandat 7.508 kb on + strandat 7.540 kb on + strandat 7.540 kb on + strandat 7.540 kb on + strandat 7.540 kb on + strandat 7.541 kb on - strandat 7.581 kb on + strandat 7.920 kb on + strand, within yubMat 7.920 kb on + strand, within yubMat 7.920 kb on + strand, within yubMat 8.004 kb on + strand, within yubMat 8.005 kb on - strand, within yubMat 8.005 kb on - strand, within yubMat 8.032 kb on - strand, within yubMat 8.484 kb on - strand, within yubMat 8.484 kb on - strand, within yubMat 8.489 kb on + strand, within yubMat 8.489 kb on + strand, within yubMat 8.489 kb on + strand, within yubMat 8.490 kb on - strand, within yubMat 8.490 kb on - strand, within yubMat 8.490 kb on - strand, within yubMat 8.490 kb on - strand, within yubMat 8.490 kb on - strand, within yubMat 8.530 kb on - strand, within yubMat 8.630 kb on + strand, within yubMat 8.631 kb on - strand, within yubMat 8.631 kb on - strand, within yubMat 8.710 kb on - strand, within yubMat 8.815 kb on + strand, within yubMat 8.815 kb on + strand, within yubMat 9.107 kb on - strand, within yubMat 9.244 kb on - strand, within yubMat 9.312 kb on + strandat 9.396 kb on + strandat 9.426 kb on + strandat 9.435 kb on + strandat 9.444 kb on + strandat 9.444 kb on + strandat 9.444 kb on + strandat 9.444 kb on + strandat 9.444 kb on + strandat 9.444 kb on + strandat 9.445 kb on - strandat 9.448 kb on + strandat 9.448 kb on + strandat 9.448 kb on + strandat 9.448 kb on + strandat 9.448 kb on + strandat 9.448 kb on + strandat 9.448 kb on + strandat 9.449 kb on - strandat 9.449 kb on - strandat 9.449 kb on - strandat 9.449 kb on - strandat 9.452 kb on - strandat 9.452 kb on - strandat 9.469 kb on + strandat 9.469 kb on + strandat 9.515 kb on + strandat 9.723 kb on + strandat 9.878 kb on + strand, within ssbat 9.879 kb on - strand, within ssbat 10.040 kb on - strand, within ssbat 10.050 kb on - strand, within ssbat 10.093 kb on + strand, within ssbat 10.118 kb on - strand, within ssbat 10.118 kb on - strand, within ssbat 10.262 kb on - strand, within ssbat 10.262 kb on - strand, within ssbat 10.334 kb on - strandat 10.334 kb on - strandat 10.334 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas09
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6,613 - psiA NOLOHH_24270 0.47 +1.0
6,756 + psiA NOLOHH_24270 0.67 +0.3
6,756 + psiA NOLOHH_24270 0.67 +1.0
6,757 - psiA NOLOHH_24270 0.67 +2.0
6,876 + psiA NOLOHH_24270 0.83 -0.9
7,020 + -0.0
7,195 + psiB NOLOHH_24275 0.47 -0.3
7,196 - psiB NOLOHH_24275 0.47 +1.5
7,196 - psiB NOLOHH_24275 0.47 +2.4
7,373 + psiB NOLOHH_24275 0.88 +2.0
7,396 + +0.4
7,396 + +1.7
7,397 - +0.5
7,397 - -0.1
7,399 + -0.2
7,399 + -0.0
7,399 + -1.0
7,508 + -0.0
7,540 + +2.0
7,540 + -0.4
7,540 + +1.5
7,540 + -1.4
7,541 - +0.7
7,581 + -1.0
7,920 + yubM NOLOHH_24280 0.22 +1.0
7,920 + yubM NOLOHH_24280 0.22 -0.0
7,920 + yubM NOLOHH_24280 0.22 -0.0
8,004 + yubM NOLOHH_24280 0.27 +1.4
8,005 - yubM NOLOHH_24280 0.27 -0.0
8,005 - yubM NOLOHH_24280 0.27 -0.0
8,032 - yubM NOLOHH_24280 0.28 +1.0
8,484 - yubM NOLOHH_24280 0.51 +1.0
8,484 - yubM NOLOHH_24280 0.51 -0.6
8,489 + yubM NOLOHH_24280 0.51 +1.0
8,489 + yubM NOLOHH_24280 0.51 +1.4
8,489 + yubM NOLOHH_24280 0.51 +1.7
8,490 - yubM NOLOHH_24280 0.51 +1.0
8,490 - yubM NOLOHH_24280 0.51 -0.5
8,490 - yubM NOLOHH_24280 0.51 +2.0
8,490 - yubM NOLOHH_24280 0.51 +0.7
8,490 - yubM NOLOHH_24280 0.51 -0.6
8,530 - yubM NOLOHH_24280 0.53 +0.4
8,630 + yubM NOLOHH_24280 0.59 -0.8
8,631 - yubM NOLOHH_24280 0.59 -0.0
8,631 - yubM NOLOHH_24280 0.59 -0.6
8,710 - yubM NOLOHH_24280 0.63 -0.9
8,815 + yubM NOLOHH_24280 0.68 +2.5
8,815 + yubM NOLOHH_24280 0.68 +1.0
9,107 - yubM NOLOHH_24280 0.83 +1.0
9,244 - yubM NOLOHH_24280 0.90 +2.5
9,312 + +0.7
9,396 + -0.0
9,426 + +1.1
9,435 + +0.6
9,444 + -0.0
9,444 + +0.5
9,444 + -0.1
9,444 + +0.5
9,444 + -0.5
9,444 + -1.6
9,445 - +1.2
9,448 + +1.0
9,448 + +1.0
9,448 + +0.1
9,448 + +0.0
9,448 + -0.0
9,448 + +1.8
9,448 + -1.5
9,449 - -0.9
9,449 - -0.0
9,449 - -1.9
9,449 - -1.4
9,452 - +1.4
9,452 - -0.2
9,469 + +0.4
9,469 + +1.5
9,515 + -0.0
9,723 + +1.0
9,878 + ssb NOLOHH_24290 0.15 -0.0
9,879 - ssb NOLOHH_24290 0.15 +1.0
10,040 - ssb NOLOHH_24290 0.45 -0.6
10,050 - ssb NOLOHH_24290 0.47 +1.5
10,093 + ssb NOLOHH_24290 0.55 -0.0
10,118 - ssb NOLOHH_24290 0.60 -0.3
10,118 - ssb NOLOHH_24290 0.60 -1.6
10,262 - ssb NOLOHH_24290 0.87 +2.0
10,262 - ssb NOLOHH_24290 0.87 -1.4
10,334 - +1.0
10,334 - +2.5
10,334 - -0.6

Or see this region's nucleotide sequence