Experiment: Bas09
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt NOLOHH_23040 and NOLOHH_23045 overlap by 4 nucleotides NOLOHH_23045 and NOLOHH_23050 are separated by 1 nucleotides NOLOHH_23050 and NOLOHH_23055 overlap by 4 nucleotides NOLOHH_23055 and NOLOHH_23060 are separated by 203 nucleotides NOLOHH_23060 and NOLOHH_23065 are separated by 88 nucleotides NOLOHH_23065 and int are separated by 235 nucleotides
NOLOHH_23040: NOLOHH_23040 - hypothetical protein, at 4,533,284 to 4,533,877
_23040
NOLOHH_23045: NOLOHH_23045 - hypothetical protein, at 4,533,874 to 4,534,113
_23045
NOLOHH_23050: NOLOHH_23050 - Ead/Ea22-like protein, at 4,534,115 to 4,534,630
_23050
NOLOHH_23055: NOLOHH_23055 - DUF550 domain-containing protein, at 4,534,627 to 4,535,343
_23055
NOLOHH_23060: NOLOHH_23060 - IS1 orfB, at 4,535,547 to 4,535,828
_23060
NOLOHH_23065: NOLOHH_23065 - hypothetical protein, at 4,535,917 to 4,536,084
_23065
NOLOHH_23070: int - Integrase, at 4,536,320 to 4,537,390
int
Position (kb)
4534
4535
4536 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 4533.654 kb on - strand, within NOLOHH_23040 at 4533.656 kb on + strand, within NOLOHH_23040 at 4533.657 kb on - strand, within NOLOHH_23040 at 4533.775 kb on + strand, within NOLOHH_23040 at 4533.775 kb on + strand, within NOLOHH_23040 at 4533.821 kb on - strand at 4533.821 kb on - strand at 4533.837 kb on + strand at 4533.837 kb on + strand at 4533.839 kb on + strand at 4533.938 kb on + strand, within NOLOHH_23045 at 4533.997 kb on + strand, within NOLOHH_23045 at 4533.998 kb on - strand, within NOLOHH_23045 at 4534.088 kb on + strand, within NOLOHH_23045 at 4534.151 kb on + strand at 4534.201 kb on + strand, within NOLOHH_23050 at 4534.201 kb on + strand, within NOLOHH_23050 at 4534.203 kb on + strand, within NOLOHH_23050 at 4534.203 kb on + strand, within NOLOHH_23050 at 4534.203 kb on + strand, within NOLOHH_23050 at 4534.204 kb on - strand, within NOLOHH_23050 at 4534.307 kb on - strand, within NOLOHH_23050 at 4534.381 kb on + strand, within NOLOHH_23050 at 4534.416 kb on + strand, within NOLOHH_23050 at 4534.416 kb on + strand, within NOLOHH_23050 at 4534.449 kb on + strand, within NOLOHH_23050 at 4534.513 kb on + strand, within NOLOHH_23050 at 4534.576 kb on + strand, within NOLOHH_23050 at 4534.584 kb on + strand at 4534.584 kb on + strand at 4534.585 kb on - strand at 4534.586 kb on + strand at 4534.586 kb on + strand at 4534.596 kb on + strand at 4534.596 kb on + strand at 4534.606 kb on + strand at 4534.606 kb on + strand at 4534.635 kb on + strand at 4534.681 kb on + strand at 4534.692 kb on + strand at 4534.938 kb on + strand, within NOLOHH_23055 at 4535.040 kb on + strand, within NOLOHH_23055 at 4535.111 kb on + strand, within NOLOHH_23055 at 4535.111 kb on + strand, within NOLOHH_23055 at 4535.156 kb on + strand, within NOLOHH_23055 at 4535.316 kb on + strand at 4535.316 kb on + strand at 4535.337 kb on + strand at 4535.337 kb on + strand at 4535.337 kb on + strand at 4535.337 kb on + strand at 4535.337 kb on + strand at 4535.337 kb on + strand at 4535.337 kb on + strand at 4535.337 kb on + strand at 4535.337 kb on + strand at 4535.337 kb on + strand at 4535.338 kb on - strand at 4535.339 kb on + strand at 4535.341 kb on + strand at 4535.342 kb on - strand at 4535.428 kb on + strand at 4535.428 kb on + strand at 4535.444 kb on + strand at 4535.714 kb on + strand, within NOLOHH_23060 at 4535.715 kb on - strand, within NOLOHH_23060 at 4535.758 kb on + strand, within NOLOHH_23060 at 4535.769 kb on + strand, within NOLOHH_23060 at 4535.842 kb on + strand at 4535.846 kb on + strand at 4535.903 kb on + strand at 4535.914 kb on + strand at 4535.914 kb on + strand at 4535.915 kb on - strand at 4535.996 kb on - strand, within NOLOHH_23065 at 4536.024 kb on - strand, within NOLOHH_23065 at 4536.038 kb on + strand, within NOLOHH_23065 at 4536.039 kb on - strand, within NOLOHH_23065 at 4536.105 kb on + strand at 4536.105 kb on + strand at 4536.127 kb on + strand at 4536.127 kb on + strand at 4536.127 kb on + strand at 4536.128 kb on - strand at 4536.128 kb on - strand at 4536.244 kb on + strand at 4536.297 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas09 remove 4,533,654 - NOLOHH_23040 0.62 +1.5 4,533,656 + NOLOHH_23040 0.63 +1.1 4,533,657 - NOLOHH_23040 0.63 +0.5 4,533,775 + NOLOHH_23040 0.83 +0.5 4,533,775 + NOLOHH_23040 0.83 -0.3 4,533,821 - -0.1 4,533,821 - -1.5 4,533,837 + -0.8 4,533,837 + +1.2 4,533,839 + -0.5 4,533,938 + NOLOHH_23045 0.27 -1.4 4,533,997 + NOLOHH_23045 0.51 +1.1 4,533,998 - NOLOHH_23045 0.52 -1.3 4,534,088 + NOLOHH_23045 0.89 -0.6 4,534,151 + +0.1 4,534,201 + NOLOHH_23050 0.17 +0.5 4,534,201 + NOLOHH_23050 0.17 -1.5 4,534,203 + NOLOHH_23050 0.17 -1.4 4,534,203 + NOLOHH_23050 0.17 -1.1 4,534,203 + NOLOHH_23050 0.17 -0.1 4,534,204 - NOLOHH_23050 0.17 -0.3 4,534,307 - NOLOHH_23050 0.37 -0.5 4,534,381 + NOLOHH_23050 0.52 -0.3 4,534,416 + NOLOHH_23050 0.58 +0.6 4,534,416 + NOLOHH_23050 0.58 +1.2 4,534,449 + NOLOHH_23050 0.65 +0.9 4,534,513 + NOLOHH_23050 0.77 -1.7 4,534,576 + NOLOHH_23050 0.89 -0.5 4,534,584 + +1.2 4,534,584 + -1.0 4,534,585 - +0.5 4,534,586 + -0.1 4,534,586 + -1.0 4,534,596 + -0.7 4,534,596 + -0.2 4,534,606 + -1.1 4,534,606 + -0.9 4,534,635 + -0.6 4,534,681 + -1.5 4,534,692 + +0.5 4,534,938 + NOLOHH_23055 0.43 -0.1 4,535,040 + NOLOHH_23055 0.58 -0.6 4,535,111 + NOLOHH_23055 0.68 -1.9 4,535,111 + NOLOHH_23055 0.68 -0.8 4,535,156 + NOLOHH_23055 0.74 +0.5 4,535,316 + -1.0 4,535,316 + -0.5 4,535,337 + +0.5 4,535,337 + +0.4 4,535,337 + -0.5 4,535,337 + +0.3 4,535,337 + +0.5 4,535,337 + -0.5 4,535,337 + +0.8 4,535,337 + -0.5 4,535,337 + +0.1 4,535,337 + -1.0 4,535,338 - +1.5 4,535,339 + -0.1 4,535,341 + -0.4 4,535,342 - -0.9 4,535,428 + -0.5 4,535,428 + +1.5 4,535,444 + -0.3 4,535,714 + NOLOHH_23060 0.59 +0.2 4,535,715 - NOLOHH_23060 0.60 -1.1 4,535,758 + NOLOHH_23060 0.75 -0.7 4,535,769 + NOLOHH_23060 0.79 -0.2 4,535,842 + -0.3 4,535,846 + +1.1 4,535,903 + -2.1 4,535,914 + +2.1 4,535,914 + +0.5 4,535,915 - -0.4 4,535,996 - NOLOHH_23065 0.47 +0.1 4,536,024 - NOLOHH_23065 0.64 -0.6 4,536,038 + NOLOHH_23065 0.72 +3.5 4,536,039 - NOLOHH_23065 0.73 +0.8 4,536,105 + -0.5 4,536,105 + -0.5 4,536,127 + -1.5 4,536,127 + +0.3 4,536,127 + -0.9 4,536,128 - -0.5 4,536,128 - +0.5 4,536,244 + +0.6 4,536,297 + -1.1
Or see this region's nucleotide sequence