Strain Fitness in Escherichia coli ECOR27 around NOLOHH_22015

Experiment: Bas09

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntlpxK and msbA overlap by 4 nucleotidesmsbA and ycaI are separated by 36 nucleotides NOLOHH_22010: lpxK - tetraacyldisaccharide 4'-kinase, at 4,333,620 to 4,334,606 lpxK NOLOHH_22015: msbA - lipid A ABC transporter ATP-binding protein/permease MsbA, at 4,334,603 to 4,336,351 msbA NOLOHH_22020: ycaI - ComEC family protein, at 4,336,388 to 4,338,652 ycaI Position (kb) 4334 4335 4336 4337Strain fitness (log2 ratio) -2 -1 0 1 2 3at 4333.621 kb on + strandat 4333.621 kb on + strandat 4333.621 kb on + strandat 4333.622 kb on - strandat 4333.622 kb on - strandat 4333.622 kb on - strandat 4333.622 kb on - strandat 4333.622 kb on - strandat 4336.437 kb on - strandat 4336.584 kb on + strandat 4336.631 kb on + strand, within ycaIat 4336.654 kb on + strand, within ycaIat 4336.819 kb on + strand, within ycaIat 4336.819 kb on + strand, within ycaIat 4336.907 kb on + strand, within ycaIat 4337.149 kb on - strand, within ycaIat 4337.207 kb on + strand, within ycaIat 4337.208 kb on - strand, within ycaI

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas09
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4,333,621 + -0.6
4,333,621 + -0.6
4,333,621 + -1.2
4,333,622 - -0.0
4,333,622 - +0.4
4,333,622 - +1.3
4,333,622 - +0.8
4,333,622 - +3.6
4,336,437 - -0.6
4,336,584 + -2.4
4,336,631 + ycaI NOLOHH_22020 0.11 +0.4
4,336,654 + ycaI NOLOHH_22020 0.12 -1.0
4,336,819 + ycaI NOLOHH_22020 0.19 +0.3
4,336,819 + ycaI NOLOHH_22020 0.19 -1.5
4,336,907 + ycaI NOLOHH_22020 0.23 -1.4
4,337,149 - ycaI NOLOHH_22020 0.34 +0.4
4,337,207 + ycaI NOLOHH_22020 0.36 -0.0
4,337,208 - ycaI NOLOHH_22020 0.36 +0.8

Or see this region's nucleotide sequence