Strain Fitness in Escherichia coli ECOR27 around NOLOHH_22005

Experiment: Bas09

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntkdsB and ycaR overlap by 4 nucleotidesycaR and ycaQ are separated by 51 nucleotidesycaQ and lpxK are separated by 36 nucleotides NOLOHH_21995: kdsB - 3-deoxy-manno-octulosonate cytidylyltransferase, at 4,331,374 to 4,332,120 kdsB NOLOHH_22000: ycaR - protein YcaR, at 4,332,117 to 4,332,299 ycaR NOLOHH_22005: ycaQ - Uncharacterized protein YcaQ, at 4,332,351 to 4,333,583 ycaQ NOLOHH_22010: lpxK - tetraacyldisaccharide 4'-kinase, at 4,333,620 to 4,334,606 lpxK Position (kb) 4332 4333 4334Strain fitness (log2 ratio) -1 0 1 2 3at 4331.376 kb on - strandat 4332.333 kb on - strandat 4332.333 kb on - strandat 4332.367 kb on - strandat 4332.528 kb on - strand, within ycaQat 4332.580 kb on - strand, within ycaQat 4332.594 kb on + strand, within ycaQat 4332.597 kb on + strand, within ycaQat 4332.644 kb on - strand, within ycaQat 4332.644 kb on - strand, within ycaQat 4332.663 kb on - strand, within ycaQat 4332.895 kb on + strand, within ycaQat 4333.080 kb on - strand, within ycaQat 4333.198 kb on - strand, within ycaQat 4333.311 kb on - strand, within ycaQat 4333.385 kb on - strand, within ycaQat 4333.414 kb on + strand, within ycaQat 4333.518 kb on + strandat 4333.621 kb on + strandat 4333.621 kb on + strandat 4333.621 kb on + strandat 4333.622 kb on - strandat 4333.622 kb on - strandat 4333.622 kb on - strandat 4333.622 kb on - strandat 4333.622 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas09
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4,331,376 - -1.0
4,332,333 - +0.8
4,332,333 - +1.4
4,332,367 - -0.3
4,332,528 - ycaQ NOLOHH_22005 0.14 -0.3
4,332,580 - ycaQ NOLOHH_22005 0.19 +0.1
4,332,594 + ycaQ NOLOHH_22005 0.20 +0.7
4,332,597 + ycaQ NOLOHH_22005 0.20 +2.2
4,332,644 - ycaQ NOLOHH_22005 0.24 +1.4
4,332,644 - ycaQ NOLOHH_22005 0.24 -1.6
4,332,663 - ycaQ NOLOHH_22005 0.25 -0.6
4,332,895 + ycaQ NOLOHH_22005 0.44 +0.4
4,333,080 - ycaQ NOLOHH_22005 0.59 -1.2
4,333,198 - ycaQ NOLOHH_22005 0.69 +0.6
4,333,311 - ycaQ NOLOHH_22005 0.78 -1.2
4,333,385 - ycaQ NOLOHH_22005 0.84 +0.2
4,333,414 + ycaQ NOLOHH_22005 0.86 -0.2
4,333,518 + -0.2
4,333,621 + -0.6
4,333,621 + -0.6
4,333,621 + -1.2
4,333,622 - -0.0
4,333,622 - +0.4
4,333,622 - +1.3
4,333,622 - +0.8
4,333,622 - +3.6

Or see this region's nucleotide sequence