Strain Fitness in Escherichia coli ECOR27 around NOLOHH_20970

Experiment: Bas09

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntycfZ and cobB are separated by 138 nucleotidescobB and nagK are separated by 15 nucleotidesnagK and lolE are separated by 28 nucleotides NOLOHH_20960: ycfZ - Inner membrane protein YcfZ, at 4,122,792 to 4,123,580 ycfZ NOLOHH_20965: cobB - NAD-dependent protein deacylase, at 4,123,719 to 4,124,540 cobB NOLOHH_20970: nagK - N-acetylglucosamine kinase, at 4,124,556 to 4,125,467 nagK NOLOHH_20975: lolE - lipoprotein-releasing ABC transporter permease subunit LolE, at 4,125,496 to 4,126,734 lolE Position (kb) 4124 4125 4126Strain fitness (log2 ratio) -3 -2 -1 0 1at 4123.938 kb on - strand, within cobBat 4124.025 kb on - strand, within cobBat 4124.106 kb on - strand, within cobBat 4124.149 kb on - strand, within cobBat 4124.151 kb on - strand, within cobBat 4124.199 kb on - strand, within cobBat 4124.373 kb on - strand, within cobBat 4124.647 kb on - strandat 4124.647 kb on - strandat 4125.249 kb on - strand, within nagKat 4125.349 kb on - strand, within nagKat 4125.362 kb on - strand, within nagKat 4125.464 kb on - strandat 4125.464 kb on - strandat 4125.484 kb on - strandat 4125.484 kb on - strandat 4125.489 kb on - strandat 4125.489 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas09
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4,123,938 - cobB NOLOHH_20965 0.27 +0.4
4,124,025 - cobB NOLOHH_20965 0.37 +0.4
4,124,106 - cobB NOLOHH_20965 0.47 +0.4
4,124,149 - cobB NOLOHH_20965 0.52 +1.0
4,124,151 - cobB NOLOHH_20965 0.53 -0.6
4,124,199 - cobB NOLOHH_20965 0.58 +0.1
4,124,373 - cobB NOLOHH_20965 0.80 +0.9
4,124,647 - -3.2
4,124,647 - +0.4
4,125,249 - nagK NOLOHH_20970 0.76 +0.4
4,125,349 - nagK NOLOHH_20970 0.87 +0.6
4,125,362 - nagK NOLOHH_20970 0.88 +0.4
4,125,464 - -1.2
4,125,464 - +1.2
4,125,484 - +0.8
4,125,484 - -0.6
4,125,489 - +0.4
4,125,489 - +0.8

Or see this region's nucleotide sequence