Strain Fitness in Escherichia coli ECOR27 around NOLOHH_20345

Experiment: Bas09

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nttrpA and yciG are separated by 380 nucleotidesyciG and yciF are separated by 85 nucleotidesyciF and yciE are separated by 45 nucleotidesyciE and ompW are separated by 59 nucleotides NOLOHH_20335: trpA - tryptophan synthase subunit alpha, at 4,004,582 to 4,005,388 trpA NOLOHH_20340: yciG - Uncharacterized protein YciG, at 4,005,769 to 4,005,948 yciG NOLOHH_20345: yciF - Protein YciF, at 4,006,034 to 4,006,534 yciF NOLOHH_20350: yciE - Protein YciE, at 4,006,580 to 4,007,086 yciE NOLOHH_20355: ompW - outer membrane protein OmpW, at 4,007,146 to 4,007,784 ompW Position (kb) 4006 4007Strain fitness (log2 ratio) -1 0 1 2at 4005.104 kb on + strand, within trpAat 4005.104 kb on + strand, within trpAat 4005.105 kb on - strand, within trpAat 4005.188 kb on + strand, within trpAat 4005.188 kb on + strand, within trpAat 4005.221 kb on - strand, within trpAat 4005.465 kb on - strandat 4005.622 kb on - strandat 4006.086 kb on + strand, within yciFat 4007.406 kb on - strand, within ompWat 4007.406 kb on - strand, within ompW

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas09
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4,005,104 + trpA NOLOHH_20335 0.65 +0.9
4,005,104 + trpA NOLOHH_20335 0.65 +2.0
4,005,105 - trpA NOLOHH_20335 0.65 -1.2
4,005,188 + trpA NOLOHH_20335 0.75 +1.1
4,005,188 + trpA NOLOHH_20335 0.75 +0.4
4,005,221 - trpA NOLOHH_20335 0.79 -0.6
4,005,465 - -0.2
4,005,622 - -0.1
4,006,086 + yciF NOLOHH_20345 0.10 +0.4
4,007,406 - ompW NOLOHH_20355 0.41 -1.4
4,007,406 - ompW NOLOHH_20355 0.41 -0.0

Or see this region's nucleotide sequence