Strain Fitness in Escherichia coli ECOR27 around NOLOHH_17670

Experiment: Bas09

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntppsA and fadK are separated by 56 nucleotidesfadK and ydiT are separated by 56 nucleotidesydiT and ydiS overlap by 4 nucleotides NOLOHH_17665: ppsA - phosphoenolpyruvate synthase, at 3,466,104 to 3,468,482 ppsA NOLOHH_17670: fadK - medium-chain fatty-acid--CoA ligase, at 3,468,539 to 3,470,185 fadK NOLOHH_17675: ydiT - Ferredoxin-like protein YdiT, at 3,470,242 to 3,470,535 ydiT NOLOHH_17680: ydiS - putative electron transfer flavoprotein-quinone oxidoreductase YdiS, at 3,470,532 to 3,471,821 ydiS Position (kb) 3468 3469 3470 3471Strain fitness (log2 ratio) -2 -1 0 1 2at 3468.055 kb on + strand, within ppsAat 3468.342 kb on + strandat 3468.342 kb on + strandat 3468.480 kb on + strandat 3468.945 kb on + strand, within fadKat 3469.304 kb on + strand, within fadKat 3469.304 kb on + strand, within fadKat 3469.320 kb on + strand, within fadKat 3469.427 kb on + strand, within fadKat 3470.028 kb on + strandat 3470.028 kb on + strandat 3470.029 kb on - strandat 3470.029 kb on - strandat 3470.034 kb on + strandat 3470.302 kb on - strand, within ydiTat 3470.388 kb on - strand, within ydiTat 3470.984 kb on + strand, within ydiS

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas09
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3,468,055 + ppsA NOLOHH_17665 0.82 +1.9
3,468,342 + +1.0
3,468,342 + +1.3
3,468,480 + -2.0
3,468,945 + fadK NOLOHH_17670 0.25 -0.3
3,469,304 + fadK NOLOHH_17670 0.46 +0.6
3,469,304 + fadK NOLOHH_17670 0.46 +1.3
3,469,320 + fadK NOLOHH_17670 0.47 +0.0
3,469,427 + fadK NOLOHH_17670 0.54 +1.3
3,470,028 + +1.3
3,470,028 + +0.3
3,470,029 - -1.3
3,470,029 - +0.3
3,470,034 + +0.6
3,470,302 - ydiT NOLOHH_17675 0.20 +1.3
3,470,388 - ydiT NOLOHH_17675 0.50 -2.0
3,470,984 + ydiS NOLOHH_17680 0.35 +0.6

Or see this region's nucleotide sequence