Experiment: Bas09
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt uvrY and uvrC overlap by 4 nucleotides uvrC and pgsA are separated by 56 nucleotides pgsA and Gly_trna are separated by 151 nucleotides Gly_trna and Cys_trna are separated by 54 nucleotides Cys_trna and Leu_trna are separated by 12 nucleotides
NOLOHH_16440: uvrY - UvrY/SirA/GacA family response regulator transcription factor, at 3,235,941 to 3,236,597
uvrY
NOLOHH_16445: uvrC - excinuclease ABC subunit UvrC, at 3,236,594 to 3,238,426
uvrC
NOLOHH_16450: pgsA - CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase, at 3,238,483 to 3,239,031
pgsA
NOLOHH_16455: Gly_trna - tRNA-Gly(gcc), at 3,239,183 to 3,239,258
_trna
NOLOHH_16460: Cys_trna - tRNA-Cys(gca), at 3,239,313 to 3,239,386
_trna
NOLOHH_16465: Leu_trna - tRNA-Leu(taa), at 3,239,399 to 3,239,485
_trna
Position (kb)
3236
3237
3238
3239 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3235.618 kb on + strand at 3235.618 kb on + strand at 3235.620 kb on + strand at 3235.620 kb on + strand at 3235.631 kb on - strand at 3235.679 kb on - strand at 3235.684 kb on + strand at 3235.756 kb on - strand at 3235.799 kb on + strand at 3235.890 kb on + strand at 3235.890 kb on + strand at 3235.900 kb on + strand at 3235.954 kb on + strand at 3236.011 kb on + strand, within uvrY at 3236.011 kb on + strand, within uvrY at 3236.310 kb on + strand, within uvrY at 3236.411 kb on + strand, within uvrY at 3236.411 kb on + strand, within uvrY at 3236.663 kb on + strand at 3236.663 kb on + strand at 3236.683 kb on + strand at 3236.683 kb on + strand at 3236.687 kb on + strand at 3236.687 kb on + strand at 3236.687 kb on + strand at 3236.688 kb on - strand at 3236.905 kb on + strand, within uvrC at 3237.061 kb on + strand, within uvrC at 3237.105 kb on + strand, within uvrC at 3237.173 kb on + strand, within uvrC at 3237.173 kb on + strand, within uvrC at 3237.438 kb on - strand, within uvrC at 3237.438 kb on - strand, within uvrC at 3237.480 kb on + strand, within uvrC at 3237.617 kb on - strand, within uvrC at 3237.883 kb on + strand, within uvrC at 3237.909 kb on - strand, within uvrC at 3237.964 kb on + strand, within uvrC at 3238.071 kb on + strand, within uvrC at 3238.147 kb on + strand, within uvrC at 3238.147 kb on + strand, within uvrC at 3238.180 kb on + strand, within uvrC at 3238.231 kb on + strand, within uvrC at 3238.316 kb on + strand at 3238.318 kb on + strand at 3238.468 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas09 remove 3,235,618 + -0.7 3,235,618 + -0.7 3,235,620 + +0.3 3,235,620 + -2.5 3,235,631 - +2.3 3,235,679 - +0.7 3,235,684 + -1.7 3,235,756 - -0.0 3,235,799 + -0.7 3,235,890 + -0.5 3,235,890 + -0.2 3,235,900 + +0.7 3,235,954 + -0.7 3,236,011 + uvrY NOLOHH_16440 0.11 -0.5 3,236,011 + uvrY NOLOHH_16440 0.11 +0.7 3,236,310 + uvrY NOLOHH_16440 0.56 +0.3 3,236,411 + uvrY NOLOHH_16440 0.72 -0.3 3,236,411 + uvrY NOLOHH_16440 0.72 +0.3 3,236,663 + -1.3 3,236,663 + -0.1 3,236,683 + -0.4 3,236,683 + +1.1 3,236,687 + -0.3 3,236,687 + -2.5 3,236,687 + -1.3 3,236,688 - -0.7 3,236,905 + uvrC NOLOHH_16445 0.17 +1.6 3,237,061 + uvrC NOLOHH_16445 0.25 +0.3 3,237,105 + uvrC NOLOHH_16445 0.28 -0.0 3,237,173 + uvrC NOLOHH_16445 0.32 +1.6 3,237,173 + uvrC NOLOHH_16445 0.32 +2.1 3,237,438 - uvrC NOLOHH_16445 0.46 -1.4 3,237,438 - uvrC NOLOHH_16445 0.46 +1.6 3,237,480 + uvrC NOLOHH_16445 0.48 -0.7 3,237,617 - uvrC NOLOHH_16445 0.56 +0.3 3,237,883 + uvrC NOLOHH_16445 0.70 +1.3 3,237,909 - uvrC NOLOHH_16445 0.72 -0.7 3,237,964 + uvrC NOLOHH_16445 0.75 +0.3 3,238,071 + uvrC NOLOHH_16445 0.81 -1.3 3,238,147 + uvrC NOLOHH_16445 0.85 +0.0 3,238,147 + uvrC NOLOHH_16445 0.85 -0.7 3,238,180 + uvrC NOLOHH_16445 0.87 +0.3 3,238,231 + uvrC NOLOHH_16445 0.89 +0.3 3,238,316 + +0.7 3,238,318 + +1.3 3,238,468 + -1.7
Or see this region's nucleotide sequence