Strain Fitness in Escherichia coli ECOR27 around NOLOHH_13870

Experiment: Bas09

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntpdxK and crr are separated by 41 nucleotidescrr and ptsI are separated by 40 nucleotidesptsI and ptsH are separated by 44 nucleotidesptsH and cysK are separated by 383 nucleotides NOLOHH_13860: pdxK - pyridoxine/pyridoxal/pyridoxamine kinase, at 2,693,323 to 2,694,174 pdxK NOLOHH_13865: crr - PTS glucose transporter subunit IIA, at 2,694,216 to 2,694,725 crr NOLOHH_13870: ptsI - phosphoenolpyruvate-protein phosphotransferase PtsI, at 2,694,766 to 2,696,493 ptsI NOLOHH_13875: ptsH - phosphocarrier protein Hpr, at 2,696,538 to 2,696,795 ptsH NOLOHH_13880: cysK - cysteine synthase A, at 2,697,179 to 2,698,150 cysK Position (kb) 2694 2695 2696 2697Strain fitness (log2 ratio) -1 0 1at 2693.888 kb on + strand, within pdxKat 2693.888 kb on + strand, within pdxKat 2694.218 kb on - strandat 2694.306 kb on - strand, within crrat 2694.744 kb on - strandat 2694.812 kb on - strandat 2694.812 kb on - strandat 2696.867 kb on - strandat 2696.867 kb on - strandat 2696.867 kb on - strandat 2697.067 kb on + strandat 2697.068 kb on - strandat 2697.140 kb on + strandat 2697.231 kb on - strandat 2697.231 kb on - strandat 2697.231 kb on - strandat 2697.300 kb on - strand, within cysKat 2697.300 kb on - strand, within cysKat 2697.338 kb on - strand, within cysKat 2697.440 kb on - strand, within cysK

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas09
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2,693,888 + pdxK NOLOHH_13860 0.66 +0.2
2,693,888 + pdxK NOLOHH_13860 0.66 -1.2
2,694,218 - +0.4
2,694,306 - crr NOLOHH_13865 0.18 -0.3
2,694,744 - -0.1
2,694,812 - -0.2
2,694,812 - +0.4
2,696,867 - -0.4
2,696,867 - +0.4
2,696,867 - -0.2
2,697,067 + -0.6
2,697,068 - +1.1
2,697,140 + +1.2
2,697,231 - -0.2
2,697,231 - +0.4
2,697,231 - -0.6
2,697,300 - cysK NOLOHH_13880 0.12 -0.9
2,697,300 - cysK NOLOHH_13880 0.12 +0.0
2,697,338 - cysK NOLOHH_13880 0.16 -0.6
2,697,440 - cysK NOLOHH_13880 0.27 +0.9

Or see this region's nucleotide sequence