Strain Fitness in Escherichia coli ECOR27 around NOLOHH_13860

Experiment: Bas09

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntcysM and yfeK are separated by 2 nucleotidesyfeK and pdxK are separated by 104 nucleotidespdxK and crr are separated by 41 nucleotidescrr and ptsI are separated by 40 nucleotides NOLOHH_13850: cysM - cysteine synthase B, at 2,691,936 to 2,692,847 cysM NOLOHH_13855: yfeK - Uncharacterized protein YfeK, at 2,692,850 to 2,693,218 yfeK NOLOHH_13860: pdxK - pyridoxine/pyridoxal/pyridoxamine kinase, at 2,693,323 to 2,694,174 pdxK NOLOHH_13865: crr - PTS glucose transporter subunit IIA, at 2,694,216 to 2,694,725 crr NOLOHH_13870: ptsI - phosphoenolpyruvate-protein phosphotransferase PtsI, at 2,694,766 to 2,696,493 ptsI Position (kb) 2693 2694 2695Strain fitness (log2 ratio) -1 0 1at 2692.361 kb on + strand, within cysMat 2692.387 kb on - strand, within cysMat 2692.490 kb on + strand, within cysMat 2692.550 kb on + strand, within cysMat 2692.550 kb on + strand, within cysMat 2692.581 kb on + strand, within cysMat 2692.581 kb on + strand, within cysMat 2692.629 kb on - strand, within cysMat 2692.827 kb on - strandat 2692.846 kb on - strandat 2692.887 kb on - strand, within yfeKat 2692.896 kb on - strand, within yfeKat 2692.943 kb on - strand, within yfeKat 2692.944 kb on + strand, within yfeKat 2693.065 kb on + strand, within yfeKat 2693.065 kb on + strand, within yfeKat 2693.066 kb on - strand, within yfeKat 2693.066 kb on - strand, within yfeKat 2693.111 kb on + strand, within yfeKat 2693.186 kb on + strandat 2693.186 kb on + strandat 2693.187 kb on - strandat 2693.192 kb on - strandat 2693.192 kb on - strandat 2693.230 kb on - strandat 2693.328 kb on + strandat 2693.500 kb on + strand, within pdxKat 2693.501 kb on - strand, within pdxKat 2693.608 kb on + strand, within pdxKat 2693.888 kb on + strand, within pdxKat 2693.888 kb on + strand, within pdxKat 2694.218 kb on - strandat 2694.306 kb on - strand, within crrat 2694.744 kb on - strandat 2694.812 kb on - strandat 2694.812 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas09
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2,692,361 + cysM NOLOHH_13850 0.47 -1.2
2,692,387 - cysM NOLOHH_13850 0.49 +0.5
2,692,490 + cysM NOLOHH_13850 0.61 -0.5
2,692,550 + cysM NOLOHH_13850 0.67 +1.4
2,692,550 + cysM NOLOHH_13850 0.67 -1.5
2,692,581 + cysM NOLOHH_13850 0.71 -0.2
2,692,581 + cysM NOLOHH_13850 0.71 +0.4
2,692,629 - cysM NOLOHH_13850 0.76 -1.2
2,692,827 - -0.6
2,692,846 - -0.5
2,692,887 - yfeK NOLOHH_13855 0.10 -1.2
2,692,896 - yfeK NOLOHH_13855 0.12 +0.0
2,692,943 - yfeK NOLOHH_13855 0.25 -0.1
2,692,944 + yfeK NOLOHH_13855 0.25 +1.4
2,693,065 + yfeK NOLOHH_13855 0.58 +0.4
2,693,065 + yfeK NOLOHH_13855 0.58 -1.2
2,693,066 - yfeK NOLOHH_13855 0.59 -0.4
2,693,066 - yfeK NOLOHH_13855 0.59 +0.5
2,693,111 + yfeK NOLOHH_13855 0.71 -0.6
2,693,186 + -0.6
2,693,186 + -0.1
2,693,187 - -1.2
2,693,192 - +1.4
2,693,192 - -0.5
2,693,230 - +0.4
2,693,328 + +0.6
2,693,500 + pdxK NOLOHH_13860 0.21 -0.1
2,693,501 - pdxK NOLOHH_13860 0.21 +0.4
2,693,608 + pdxK NOLOHH_13860 0.33 +1.4
2,693,888 + pdxK NOLOHH_13860 0.66 +0.2
2,693,888 + pdxK NOLOHH_13860 0.66 -1.2
2,694,218 - +0.4
2,694,306 - crr NOLOHH_13865 0.18 -0.3
2,694,744 - -0.1
2,694,812 - -0.2
2,694,812 - +0.4

Or see this region's nucleotide sequence