Strain Fitness in Escherichia coli ECOR27 around NOLOHH_02840

Experiment: Bas09

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntlpdA and aceF are separated by 324 nucleotidesaceF and aceE are separated by 14 nucleotides NOLOHH_02835: lpdA - dihydrolipoyl dehydrogenase, at 480,010 to 481,434 lpdA NOLOHH_02840: aceF - pyruvate dehydrogenase complex dihydrolipoyllysine-residue acetyltransferase, at 481,759 to 483,651 aceF NOLOHH_02845: aceE - pyruvate dehydrogenase (acetyl-transferring), homodimeric type, at 483,666 to 486,329 aceE Position (kb) 481 482 483 484Strain fitness (log2 ratio) -1 0 1at 481.598 kb on - strandat 481.601 kb on - strandat 481.757 kb on - strandat 481.760 kb on + strandat 481.761 kb on - strandat 483.233 kb on - strand, within aceFat 483.668 kb on - strandat 483.788 kb on - strandat 483.887 kb on - strandat 483.887 kb on - strandat 483.918 kb on - strandat 484.058 kb on + strand, within aceEat 484.059 kb on - strand, within aceEat 484.190 kb on + strand, within aceEat 484.265 kb on - strand, within aceEat 484.362 kb on - strand, within aceEat 484.534 kb on + strand, within aceEat 484.534 kb on + strand, within aceEat 484.534 kb on + strand, within aceE

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas09
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481,598 - -0.5
481,601 - +0.5
481,757 - +0.9
481,760 + +0.1
481,761 - +1.1
483,233 - aceF NOLOHH_02840 0.78 +0.5
483,668 - -0.5
483,788 - +0.5
483,887 - +0.5
483,887 - -0.5
483,918 - +0.5
484,058 + aceE NOLOHH_02845 0.15 +0.5
484,059 - aceE NOLOHH_02845 0.15 +0.5
484,190 + aceE NOLOHH_02845 0.20 +0.5
484,265 - aceE NOLOHH_02845 0.22 +0.5
484,362 - aceE NOLOHH_02845 0.26 +0.5
484,534 + aceE NOLOHH_02845 0.33 +0.5
484,534 + aceE NOLOHH_02845 0.33 +0.5
484,534 + aceE NOLOHH_02845 0.33 +0.5

Or see this region's nucleotide sequence