Strain Fitness in Escherichia coli ECOR27 around NOLOHH_02325

Experiment: Bas09

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntrnhA and yafS overlap by 4 nucleotidesyafS and gloB are separated by 33 nucleotidesgloB and mltD are separated by 209 nucleotides NOLOHH_02315: rnhA - ribonuclease HI, at 372,935 to 373,402 rnhA NOLOHH_02320: yafS - Uncharacterized protein YafS, at 373,399 to 374,121 yafS NOLOHH_02325: gloB - hydroxyacylglutathione hydrolase, at 374,155 to 374,910 gloB NOLOHH_02330: mltD - murein transglycosylase D, at 375,120 to 376,340 mltD Position (kb) 374 375Strain fitness (log2 ratio) -2 -1 0 1 2 3at 373.165 kb on + strand, within rnhAat 373.166 kb on - strand, within rnhAat 373.183 kb on + strand, within rnhAat 373.184 kb on - strand, within rnhAat 373.400 kb on + strandat 373.413 kb on + strandat 373.414 kb on - strandat 373.465 kb on - strandat 373.518 kb on + strand, within yafSat 373.518 kb on + strand, within yafSat 373.518 kb on + strand, within yafSat 373.518 kb on + strand, within yafSat 373.519 kb on - strand, within yafSat 373.525 kb on + strand, within yafSat 373.525 kb on + strand, within yafSat 373.525 kb on + strand, within yafSat 373.525 kb on + strand, within yafSat 373.525 kb on + strand, within yafSat 373.526 kb on - strand, within yafSat 373.526 kb on - strand, within yafSat 373.545 kb on + strand, within yafSat 373.558 kb on + strand, within yafSat 373.660 kb on - strand, within yafSat 373.674 kb on + strand, within yafSat 373.674 kb on + strand, within yafSat 373.675 kb on - strand, within yafSat 373.676 kb on + strand, within yafSat 373.676 kb on + strand, within yafSat 373.677 kb on - strand, within yafSat 373.827 kb on + strand, within yafSat 373.827 kb on + strand, within yafSat 373.827 kb on + strand, within yafSat 373.827 kb on + strand, within yafSat 373.827 kb on + strand, within yafSat 373.827 kb on + strand, within yafSat 373.827 kb on + strand, within yafSat 373.827 kb on + strand, within yafSat 373.827 kb on + strand, within yafSat 373.827 kb on + strand, within yafSat 373.827 kb on + strand, within yafSat 373.828 kb on - strand, within yafSat 373.828 kb on - strand, within yafSat 373.828 kb on - strand, within yafSat 373.828 kb on - strand, within yafSat 373.828 kb on - strand, within yafSat 373.828 kb on - strand, within yafSat 373.828 kb on - strand, within yafSat 373.984 kb on - strand, within yafSat 374.142 kb on + strandat 374.142 kb on + strandat 374.143 kb on - strandat 374.149 kb on + strandat 374.150 kb on - strandat 374.150 kb on - strandat 374.150 kb on - strandat 374.154 kb on + strandat 374.154 kb on + strandat 374.155 kb on - strandat 374.169 kb on + strandat 374.170 kb on - strandat 374.170 kb on - strandat 374.170 kb on - strandat 374.191 kb on + strandat 374.191 kb on + strandat 374.191 kb on + strandat 374.191 kb on + strandat 374.191 kb on + strandat 374.191 kb on + strandat 374.191 kb on + strandat 374.191 kb on + strandat 374.192 kb on - strandat 374.192 kb on - strandat 374.261 kb on + strand, within gloBat 374.300 kb on + strand, within gloBat 374.300 kb on + strand, within gloBat 374.300 kb on + strand, within gloBat 374.301 kb on - strand, within gloBat 374.377 kb on + strand, within gloBat 374.377 kb on + strand, within gloBat 374.418 kb on - strand, within gloBat 374.465 kb on + strand, within gloBat 374.465 kb on + strand, within gloBat 374.466 kb on - strand, within gloBat 374.490 kb on - strand, within gloBat 374.496 kb on + strand, within gloBat 374.496 kb on + strand, within gloBat 374.497 kb on - strand, within gloBat 374.504 kb on - strand, within gloBat 374.518 kb on + strand, within gloBat 374.560 kb on - strand, within gloBat 374.605 kb on - strand, within gloBat 374.636 kb on + strand, within gloBat 374.636 kb on + strand, within gloBat 374.637 kb on - strand, within gloBat 374.655 kb on + strand, within gloBat 374.655 kb on + strand, within gloBat 374.656 kb on - strand, within gloBat 374.656 kb on - strand, within gloBat 374.756 kb on + strand, within gloBat 374.757 kb on - strand, within gloBat 374.961 kb on + strandat 374.969 kb on + strandat 374.969 kb on + strandat 374.998 kb on + strandat 375.101 kb on + strandat 375.137 kb on + strandat 375.138 kb on - strandat 375.231 kb on + strandat 375.242 kb on + strand, within mltDat 375.242 kb on + strand, within mltDat 375.249 kb on + strand, within mltDat 375.249 kb on + strand, within mltDat 375.250 kb on - strand, within mltDat 375.250 kb on - strand, within mltDat 375.262 kb on + strand, within mltDat 375.262 kb on + strand, within mltDat 375.262 kb on + strand, within mltDat 375.262 kb on + strand, within mltDat 375.263 kb on - strand, within mltDat 375.282 kb on + strand, within mltDat 375.282 kb on + strand, within mltDat 375.282 kb on + strand, within mltDat 375.283 kb on - strand, within mltDat 375.284 kb on + strand, within mltDat 375.284 kb on + strand, within mltDat 375.284 kb on + strand, within mltDat 375.284 kb on + strand, within mltDat 375.284 kb on + strand, within mltDat 375.285 kb on - strand, within mltDat 375.285 kb on - strand, within mltDat 375.285 kb on - strand, within mltDat 375.288 kb on + strand, within mltDat 375.288 kb on + strand, within mltDat 375.288 kb on + strand, within mltDat 375.340 kb on + strand, within mltDat 375.340 kb on + strand, within mltDat 375.340 kb on + strand, within mltDat 375.343 kb on + strand, within mltDat 375.343 kb on + strand, within mltDat 375.343 kb on + strand, within mltDat 375.435 kb on + strand, within mltDat 375.506 kb on + strand, within mltDat 375.507 kb on - strand, within mltDat 375.566 kb on + strand, within mltDat 375.566 kb on + strand, within mltDat 375.599 kb on + strand, within mltDat 375.669 kb on + strand, within mltDat 375.669 kb on + strand, within mltDat 375.669 kb on + strand, within mltDat 375.669 kb on + strand, within mltDat 375.670 kb on - strand, within mltDat 375.677 kb on + strand, within mltDat 375.699 kb on - strand, within mltDat 375.727 kb on + strand, within mltDat 375.809 kb on + strand, within mltDat 375.809 kb on + strand, within mltDat 375.891 kb on + strand, within mltD

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas09
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373,165 + rnhA NOLOHH_02315 0.49 -0.9
373,166 - rnhA NOLOHH_02315 0.49 -1.2
373,183 + rnhA NOLOHH_02315 0.53 -0.6
373,184 - rnhA NOLOHH_02315 0.53 -0.7
373,400 + -0.6
373,413 + -1.2
373,414 - -0.7
373,465 - -1.2
373,518 + yafS NOLOHH_02320 0.16 -0.6
373,518 + yafS NOLOHH_02320 0.16 -0.6
373,518 + yafS NOLOHH_02320 0.16 -0.2
373,518 + yafS NOLOHH_02320 0.16 +0.4
373,519 - yafS NOLOHH_02320 0.17 +1.4
373,525 + yafS NOLOHH_02320 0.17 -0.6
373,525 + yafS NOLOHH_02320 0.17 +0.9
373,525 + yafS NOLOHH_02320 0.17 +1.1
373,525 + yafS NOLOHH_02320 0.17 -0.6
373,525 + yafS NOLOHH_02320 0.17 +0.4
373,526 - yafS NOLOHH_02320 0.18 -1.2
373,526 - yafS NOLOHH_02320 0.18 -1.0
373,545 + yafS NOLOHH_02320 0.20 +1.0
373,558 + yafS NOLOHH_02320 0.22 -0.6
373,660 - yafS NOLOHH_02320 0.36 -0.2
373,674 + yafS NOLOHH_02320 0.38 +0.8
373,674 + yafS NOLOHH_02320 0.38 -0.2
373,675 - yafS NOLOHH_02320 0.38 -0.1
373,676 + yafS NOLOHH_02320 0.38 +0.4
373,676 + yafS NOLOHH_02320 0.38 -0.3
373,677 - yafS NOLOHH_02320 0.38 +0.7
373,827 + yafS NOLOHH_02320 0.59 +0.6
373,827 + yafS NOLOHH_02320 0.59 +0.3
373,827 + yafS NOLOHH_02320 0.59 +0.5
373,827 + yafS NOLOHH_02320 0.59 -0.2
373,827 + yafS NOLOHH_02320 0.59 +1.2
373,827 + yafS NOLOHH_02320 0.59 +2.4
373,827 + yafS NOLOHH_02320 0.59 +1.1
373,827 + yafS NOLOHH_02320 0.59 -0.8
373,827 + yafS NOLOHH_02320 0.59 +0.4
373,827 + yafS NOLOHH_02320 0.59 -0.6
373,827 + yafS NOLOHH_02320 0.59 +0.3
373,828 - yafS NOLOHH_02320 0.59 -0.7
373,828 - yafS NOLOHH_02320 0.59 +0.7
373,828 - yafS NOLOHH_02320 0.59 +0.1
373,828 - yafS NOLOHH_02320 0.59 +0.4
373,828 - yafS NOLOHH_02320 0.59 -1.2
373,828 - yafS NOLOHH_02320 0.59 -1.4
373,828 - yafS NOLOHH_02320 0.59 +2.4
373,984 - yafS NOLOHH_02320 0.81 -0.4
374,142 + -0.2
374,142 + -1.0
374,143 - +0.8
374,149 + -0.3
374,150 - -1.7
374,150 - -0.9
374,150 - +1.0
374,154 + +1.0
374,154 + -0.2
374,155 - -0.2
374,169 + -0.5
374,170 - -2.2
374,170 - +0.2
374,170 - -0.6
374,191 + +0.4
374,191 + -0.6
374,191 + +0.2
374,191 + -0.2
374,191 + -0.7
374,191 + -1.2
374,191 + -0.3
374,191 + -0.7
374,192 - +1.4
374,192 - -1.0
374,261 + gloB NOLOHH_02325 0.14 -1.9
374,300 + gloB NOLOHH_02325 0.19 +1.0
374,300 + gloB NOLOHH_02325 0.19 +1.0
374,300 + gloB NOLOHH_02325 0.19 +1.3
374,301 - gloB NOLOHH_02325 0.19 -0.8
374,377 + gloB NOLOHH_02325 0.29 -1.2
374,377 + gloB NOLOHH_02325 0.29 +1.7
374,418 - gloB NOLOHH_02325 0.35 -0.4
374,465 + gloB NOLOHH_02325 0.41 -1.3
374,465 + gloB NOLOHH_02325 0.41 -0.6
374,466 - gloB NOLOHH_02325 0.41 -0.2
374,490 - gloB NOLOHH_02325 0.44 -1.2
374,496 + gloB NOLOHH_02325 0.45 +1.1
374,496 + gloB NOLOHH_02325 0.45 +2.8
374,497 - gloB NOLOHH_02325 0.45 -0.4
374,504 - gloB NOLOHH_02325 0.46 -0.2
374,518 + gloB NOLOHH_02325 0.48 -0.9
374,560 - gloB NOLOHH_02325 0.54 -0.6
374,605 - gloB NOLOHH_02325 0.60 +1.2
374,636 + gloB NOLOHH_02325 0.64 -0.2
374,636 + gloB NOLOHH_02325 0.64 -1.0
374,637 - gloB NOLOHH_02325 0.64 +2.4
374,655 + gloB NOLOHH_02325 0.66 -0.6
374,655 + gloB NOLOHH_02325 0.66 +0.4
374,656 - gloB NOLOHH_02325 0.66 -2.2
374,656 - gloB NOLOHH_02325 0.66 -0.6
374,756 + gloB NOLOHH_02325 0.79 +0.4
374,757 - gloB NOLOHH_02325 0.80 -0.1
374,961 + -1.3
374,969 + -0.9
374,969 + +0.2
374,998 + -1.4
375,101 + +0.4
375,137 + -0.9
375,138 - +0.0
375,231 + -0.6
375,242 + mltD NOLOHH_02330 0.10 +0.1
375,242 + mltD NOLOHH_02330 0.10 -1.1
375,249 + mltD NOLOHH_02330 0.11 +1.1
375,249 + mltD NOLOHH_02330 0.11 +0.4
375,250 - mltD NOLOHH_02330 0.11 +0.4
375,250 - mltD NOLOHH_02330 0.11 -0.6
375,262 + mltD NOLOHH_02330 0.12 -1.6
375,262 + mltD NOLOHH_02330 0.12 -0.4
375,262 + mltD NOLOHH_02330 0.12 +0.2
375,262 + mltD NOLOHH_02330 0.12 -1.2
375,263 - mltD NOLOHH_02330 0.12 -0.6
375,282 + mltD NOLOHH_02330 0.13 -2.2
375,282 + mltD NOLOHH_02330 0.13 +2.0
375,282 + mltD NOLOHH_02330 0.13 -0.2
375,283 - mltD NOLOHH_02330 0.13 +0.4
375,284 + mltD NOLOHH_02330 0.13 -1.1
375,284 + mltD NOLOHH_02330 0.13 +0.1
375,284 + mltD NOLOHH_02330 0.13 -1.4
375,284 + mltD NOLOHH_02330 0.13 -0.4
375,284 + mltD NOLOHH_02330 0.13 +0.3
375,285 - mltD NOLOHH_02330 0.14 +0.0
375,285 - mltD NOLOHH_02330 0.14 -0.6
375,285 - mltD NOLOHH_02330 0.14 +0.8
375,288 + mltD NOLOHH_02330 0.14 -0.5
375,288 + mltD NOLOHH_02330 0.14 +1.4
375,288 + mltD NOLOHH_02330 0.14 +1.0
375,340 + mltD NOLOHH_02330 0.18 -1.8
375,340 + mltD NOLOHH_02330 0.18 -1.1
375,340 + mltD NOLOHH_02330 0.18 -0.4
375,343 + mltD NOLOHH_02330 0.18 +0.3
375,343 + mltD NOLOHH_02330 0.18 -1.3
375,343 + mltD NOLOHH_02330 0.18 -1.1
375,435 + mltD NOLOHH_02330 0.26 -1.6
375,506 + mltD NOLOHH_02330 0.32 +0.0
375,507 - mltD NOLOHH_02330 0.32 -1.9
375,566 + mltD NOLOHH_02330 0.37 -1.0
375,566 + mltD NOLOHH_02330 0.37 -1.3
375,599 + mltD NOLOHH_02330 0.39 +0.3
375,669 + mltD NOLOHH_02330 0.45 +0.2
375,669 + mltD NOLOHH_02330 0.45 -0.5
375,669 + mltD NOLOHH_02330 0.45 -1.3
375,669 + mltD NOLOHH_02330 0.45 -0.0
375,670 - mltD NOLOHH_02330 0.45 +0.4
375,677 + mltD NOLOHH_02330 0.46 -1.2
375,699 - mltD NOLOHH_02330 0.47 -1.2
375,727 + mltD NOLOHH_02330 0.50 -1.4
375,809 + mltD NOLOHH_02330 0.56 +0.1
375,809 + mltD NOLOHH_02330 0.56 -1.3
375,891 + mltD NOLOHH_02330 0.63 -1.0

Or see this region's nucleotide sequence