Strain Fitness in Escherichia coli ECOR27 around NOLOHH_02305

Experiment: Bas09

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyafT and Asp_trna are separated by 326 nucleotidesAsp_trna and dnaQ are separated by 132 nucleotidesdnaQ and rnhA are separated by 55 nucleotides NOLOHH_02300: yafT - Uncharacterized lipoprotein YafT, at 370,818 to 371,603 yafT NOLOHH_02305: Asp_trna - tRNA-Asp(gtc), at 371,930 to 372,006 _trna NOLOHH_02310: dnaQ - DNA polymerase III subunit epsilon, at 372,139 to 372,879 dnaQ NOLOHH_02315: rnhA - ribonuclease HI, at 372,935 to 373,402 rnhA Position (kb) 371 372 373Strain fitness (log2 ratio) -1 0 1at 370.946 kb on + strand, within yafTat 370.948 kb on + strand, within yafTat 370.954 kb on + strand, within yafTat 371.070 kb on + strand, within yafTat 371.670 kb on - strandat 372.039 kb on + strandat 372.882 kb on - strandat 372.882 kb on - strandat 372.901 kb on - strandat 372.901 kb on - strandat 372.917 kb on - strandat 372.941 kb on - strandat 372.941 kb on - strandat 372.941 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas09
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370,946 + yafT NOLOHH_02300 0.16 -0.8
370,948 + yafT NOLOHH_02300 0.17 +0.4
370,954 + yafT NOLOHH_02300 0.17 +0.4
371,070 + yafT NOLOHH_02300 0.32 -0.2
371,670 - -0.0
372,039 + -0.9
372,882 - -1.2
372,882 - +1.0
372,901 - +0.0
372,901 - +0.0
372,917 - +0.4
372,941 - -1.0
372,941 - +1.2
372,941 - -1.2

Or see this region's nucleotide sequence