Experiment: Tween 20 (C), 30C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt A4249_RS04650 and A4249_RS16245 are separated by 31 nucleotides A4249_RS16245 and A4249_RS04655 are separated by 103 nucleotides A4249_RS04655 and A4249_RS04660 are separated by 104 nucleotides A4249_RS04660 and A4249_RS04665 are separated by 36 nucleotides
A4249_RS04650: A4249_RS04650 - protein-L-isoaspartate(D-aspartate) O-methyltransferase, at 966,217 to 966,861
_RS04650
A4249_RS16245: A4249_RS16245 - OmpA family protein, at 966,893 to 967,597
_RS16245
A4249_RS04655: A4249_RS04655 - peptidoglycan DD-metalloendopeptidase family protein, at 967,701 to 968,780
_RS04655
A4249_RS04660: A4249_RS04660 - preprotein translocase subunit YajC, at 968,885 to 969,196
_RS04660
A4249_RS04665: A4249_RS04665 - protein translocase subunit SecD, at 969,233 to 970,825
_RS04665
Position (kb)
967
968
969 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 966.966 kb on + strand, within A4249_RS16245 at 966.966 kb on + strand, within A4249_RS16245 at 966.967 kb on - strand, within A4249_RS16245 at 966.990 kb on + strand, within A4249_RS16245 at 966.990 kb on + strand, within A4249_RS16245 at 967.004 kb on + strand, within A4249_RS16245 at 967.005 kb on - strand, within A4249_RS16245 at 967.088 kb on + strand, within A4249_RS16245 at 967.088 kb on + strand, within A4249_RS16245 at 967.088 kb on + strand, within A4249_RS16245 at 967.088 kb on + strand, within A4249_RS16245 at 967.089 kb on - strand, within A4249_RS16245 at 967.089 kb on - strand, within A4249_RS16245 at 967.089 kb on - strand, within A4249_RS16245 at 967.089 kb on - strand, within A4249_RS16245 at 967.089 kb on - strand, within A4249_RS16245 at 967.089 kb on - strand, within A4249_RS16245 at 967.090 kb on + strand, within A4249_RS16245 at 967.090 kb on + strand, within A4249_RS16245 at 967.090 kb on + strand, within A4249_RS16245 at 967.090 kb on + strand, within A4249_RS16245 at 967.090 kb on + strand, within A4249_RS16245 at 967.090 kb on + strand, within A4249_RS16245 at 967.090 kb on + strand, within A4249_RS16245 at 967.091 kb on - strand, within A4249_RS16245 at 967.091 kb on - strand, within A4249_RS16245 at 967.091 kb on - strand, within A4249_RS16245 at 967.091 kb on - strand, within A4249_RS16245 at 967.091 kb on - strand, within A4249_RS16245 at 967.091 kb on - strand, within A4249_RS16245 at 967.102 kb on + strand, within A4249_RS16245 at 967.135 kb on + strand, within A4249_RS16245 at 967.135 kb on + strand, within A4249_RS16245 at 967.170 kb on + strand, within A4249_RS16245 at 967.170 kb on + strand, within A4249_RS16245 at 967.170 kb on + strand, within A4249_RS16245 at 967.170 kb on + strand, within A4249_RS16245 at 967.171 kb on - strand, within A4249_RS16245 at 967.176 kb on + strand, within A4249_RS16245 at 967.176 kb on + strand, within A4249_RS16245 at 967.176 kb on + strand, within A4249_RS16245 at 967.176 kb on + strand, within A4249_RS16245 at 967.177 kb on - strand, within A4249_RS16245 at 967.177 kb on - strand, within A4249_RS16245 at 967.177 kb on - strand, within A4249_RS16245 at 967.177 kb on - strand, within A4249_RS16245 at 967.187 kb on + strand, within A4249_RS16245 at 967.187 kb on + strand, within A4249_RS16245 at 967.188 kb on - strand, within A4249_RS16245 at 967.188 kb on - strand, within A4249_RS16245 at 967.265 kb on + strand, within A4249_RS16245 at 967.265 kb on + strand, within A4249_RS16245 at 967.265 kb on + strand, within A4249_RS16245 at 967.265 kb on + strand, within A4249_RS16245 at 967.265 kb on + strand, within A4249_RS16245 at 967.265 kb on + strand, within A4249_RS16245 at 967.265 kb on + strand, within A4249_RS16245 at 967.266 kb on - strand, within A4249_RS16245 at 967.266 kb on - strand, within A4249_RS16245 at 967.266 kb on - strand, within A4249_RS16245 at 967.266 kb on - strand, within A4249_RS16245 at 967.266 kb on - strand, within A4249_RS16245 at 967.266 kb on - strand, within A4249_RS16245 at 967.266 kb on - strand, within A4249_RS16245 at 967.335 kb on - strand, within A4249_RS16245 at 967.346 kb on + strand, within A4249_RS16245 at 967.346 kb on + strand, within A4249_RS16245 at 967.346 kb on + strand, within A4249_RS16245 at 967.346 kb on + strand, within A4249_RS16245 at 967.346 kb on + strand, within A4249_RS16245 at 967.347 kb on - strand, within A4249_RS16245 at 967.347 kb on - strand, within A4249_RS16245 at 967.347 kb on - strand, within A4249_RS16245 at 967.347 kb on - strand, within A4249_RS16245 at 967.347 kb on - strand, within A4249_RS16245 at 967.347 kb on - strand, within A4249_RS16245 at 967.347 kb on - strand, within A4249_RS16245 at 967.379 kb on + strand, within A4249_RS16245 at 967.380 kb on - strand, within A4249_RS16245 at 967.380 kb on - strand, within A4249_RS16245 at 967.387 kb on + strand, within A4249_RS16245 at 967.387 kb on + strand, within A4249_RS16245 at 967.549 kb on + strand at 967.549 kb on + strand at 967.549 kb on + strand at 967.549 kb on + strand at 967.550 kb on - strand at 967.550 kb on - strand at 967.550 kb on - strand at 967.637 kb on + strand at 967.637 kb on + strand at 967.637 kb on + strand at 967.638 kb on - strand at 967.638 kb on - strand at 967.770 kb on + strand at 967.770 kb on + strand at 967.771 kb on - strand at 967.771 kb on - strand at 967.771 kb on - strand at 967.818 kb on + strand, within A4249_RS04655 at 967.818 kb on + strand, within A4249_RS04655 at 967.818 kb on + strand, within A4249_RS04655 at 967.818 kb on + strand, within A4249_RS04655 at 967.857 kb on + strand, within A4249_RS04655 at 967.891 kb on - strand, within A4249_RS04655 at 967.905 kb on + strand, within A4249_RS04655 at 967.905 kb on + strand, within A4249_RS04655 at 967.998 kb on + strand, within A4249_RS04655 at 967.998 kb on + strand, within A4249_RS04655 at 967.999 kb on - strand, within A4249_RS04655 at 967.999 kb on - strand, within A4249_RS04655 at 968.028 kb on + strand, within A4249_RS04655 at 968.028 kb on + strand, within A4249_RS04655 at 968.030 kb on + strand, within A4249_RS04655 at 968.031 kb on - strand, within A4249_RS04655 at 968.044 kb on - strand, within A4249_RS04655 at 968.082 kb on + strand, within A4249_RS04655 at 968.082 kb on + strand, within A4249_RS04655 at 968.082 kb on + strand, within A4249_RS04655 at 968.082 kb on + strand, within A4249_RS04655 at 968.083 kb on - strand, within A4249_RS04655 at 968.083 kb on - strand, within A4249_RS04655 at 968.083 kb on - strand, within A4249_RS04655 at 968.083 kb on - strand, within A4249_RS04655 at 968.084 kb on + strand, within A4249_RS04655 at 968.084 kb on + strand, within A4249_RS04655 at 968.084 kb on + strand, within A4249_RS04655 at 968.085 kb on - strand, within A4249_RS04655 at 968.085 kb on - strand, within A4249_RS04655 at 968.085 kb on - strand, within A4249_RS04655 at 968.085 kb on - strand, within A4249_RS04655 at 968.245 kb on - strand, within A4249_RS04655 at 968.532 kb on + strand, within A4249_RS04655 at 968.532 kb on + strand, within A4249_RS04655 at 968.533 kb on - strand, within A4249_RS04655 at 968.533 kb on - strand, within A4249_RS04655 at 968.533 kb on - strand, within A4249_RS04655 at 968.533 kb on - strand, within A4249_RS04655 at 968.604 kb on + strand, within A4249_RS04655 at 968.604 kb on + strand, within A4249_RS04655 at 968.604 kb on + strand, within A4249_RS04655 at 968.674 kb on + strand at 968.674 kb on + strand at 968.674 kb on + strand at 968.675 kb on - strand at 968.675 kb on - strand at 968.778 kb on + strand at 968.778 kb on + strand at 968.779 kb on - strand at 968.779 kb on - strand at 968.779 kb on - strand at 969.197 kb on + strand at 969.217 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Tween 20 (C), 30C remove 966,966 + A4249_RS16245 0.10 -0.9 966,966 + A4249_RS16245 0.10 +0.2 966,967 - A4249_RS16245 0.10 -0.8 966,990 + A4249_RS16245 0.14 -0.3 966,990 + A4249_RS16245 0.14 +1.0 967,004 + A4249_RS16245 0.16 -0.8 967,005 - A4249_RS16245 0.16 +1.4 967,088 + A4249_RS16245 0.28 +0.1 967,088 + A4249_RS16245 0.28 -1.8 967,088 + A4249_RS16245 0.28 +1.2 967,088 + A4249_RS16245 0.28 +0.2 967,089 - A4249_RS16245 0.28 -0.8 967,089 - A4249_RS16245 0.28 -0.8 967,089 - A4249_RS16245 0.28 +0.4 967,089 - A4249_RS16245 0.28 -0.2 967,089 - A4249_RS16245 0.28 -0.3 967,089 - A4249_RS16245 0.28 -0.8 967,090 + A4249_RS16245 0.28 -0.6 967,090 + A4249_RS16245 0.28 -0.0 967,090 + A4249_RS16245 0.28 +0.4 967,090 + A4249_RS16245 0.28 +1.7 967,090 + A4249_RS16245 0.28 -2.5 967,090 + A4249_RS16245 0.28 +0.2 967,090 + A4249_RS16245 0.28 -1.2 967,091 - A4249_RS16245 0.28 +0.2 967,091 - A4249_RS16245 0.28 -0.1 967,091 - A4249_RS16245 0.28 +0.2 967,091 - A4249_RS16245 0.28 -0.4 967,091 - A4249_RS16245 0.28 +0.3 967,091 - A4249_RS16245 0.28 -0.3 967,102 + A4249_RS16245 0.30 -0.8 967,135 + A4249_RS16245 0.34 +0.3 967,135 + A4249_RS16245 0.34 -0.1 967,170 + A4249_RS16245 0.39 -0.8 967,170 + A4249_RS16245 0.39 +1.0 967,170 + A4249_RS16245 0.39 +0.5 967,170 + A4249_RS16245 0.39 +1.2 967,171 - A4249_RS16245 0.39 -3.6 967,176 + A4249_RS16245 0.40 +0.2 967,176 + A4249_RS16245 0.40 +0.3 967,176 + A4249_RS16245 0.40 +2.2 967,176 + A4249_RS16245 0.40 -0.2 967,177 - A4249_RS16245 0.40 -1.0 967,177 - A4249_RS16245 0.40 -0.5 967,177 - A4249_RS16245 0.40 +0.2 967,177 - A4249_RS16245 0.40 -1.7 967,187 + A4249_RS16245 0.42 -0.4 967,187 + A4249_RS16245 0.42 -1.0 967,188 - A4249_RS16245 0.42 +0.2 967,188 - A4249_RS16245 0.42 -3.2 967,265 + A4249_RS16245 0.53 -0.8 967,265 + A4249_RS16245 0.53 +1.2 967,265 + A4249_RS16245 0.53 +1.2 967,265 + A4249_RS16245 0.53 +1.9 967,265 + A4249_RS16245 0.53 +0.7 967,265 + A4249_RS16245 0.53 +0.2 967,265 + A4249_RS16245 0.53 -0.3 967,266 - A4249_RS16245 0.53 +1.0 967,266 - A4249_RS16245 0.53 -0.7 967,266 - A4249_RS16245 0.53 +0.2 967,266 - A4249_RS16245 0.53 +0.2 967,266 - A4249_RS16245 0.53 -0.3 967,266 - A4249_RS16245 0.53 -0.1 967,266 - A4249_RS16245 0.53 +2.1 967,335 - A4249_RS16245 0.63 -0.7 967,346 + A4249_RS16245 0.64 +1.3 967,346 + A4249_RS16245 0.64 +0.3 967,346 + A4249_RS16245 0.64 +0.6 967,346 + A4249_RS16245 0.64 -2.4 967,346 + A4249_RS16245 0.64 +2.7 967,347 - A4249_RS16245 0.64 +0.3 967,347 - A4249_RS16245 0.64 -0.2 967,347 - A4249_RS16245 0.64 +0.5 967,347 - A4249_RS16245 0.64 -1.8 967,347 - A4249_RS16245 0.64 +0.8 967,347 - A4249_RS16245 0.64 -0.7 967,347 - A4249_RS16245 0.64 -0.1 967,379 + A4249_RS16245 0.69 +0.7 967,380 - A4249_RS16245 0.69 +0.2 967,380 - A4249_RS16245 0.69 +0.2 967,387 + A4249_RS16245 0.70 +0.2 967,387 + A4249_RS16245 0.70 -1.3 967,549 + +0.2 967,549 + +0.1 967,549 + -0.5 967,549 + +0.9 967,550 - +0.3 967,550 - -0.1 967,550 - +0.4 967,637 + +0.2 967,637 + -0.6 967,637 + -1.0 967,638 - -1.0 967,638 - +0.7 967,770 + -3.4 967,770 + -0.7 967,771 - -0.3 967,771 - -0.8 967,771 - -0.6 967,818 + A4249_RS04655 0.11 -0.6 967,818 + A4249_RS04655 0.11 -0.8 967,818 + A4249_RS04655 0.11 -0.4 967,818 + A4249_RS04655 0.11 -2.2 967,857 + A4249_RS04655 0.14 -1.3 967,891 - A4249_RS04655 0.18 +0.2 967,905 + A4249_RS04655 0.19 -1.2 967,905 + A4249_RS04655 0.19 +0.2 967,998 + A4249_RS04655 0.28 +0.2 967,998 + A4249_RS04655 0.28 -1.6 967,999 - A4249_RS04655 0.28 -2.2 967,999 - A4249_RS04655 0.28 -0.8 968,028 + A4249_RS04655 0.30 -0.8 968,028 + A4249_RS04655 0.30 -1.3 968,030 + A4249_RS04655 0.30 +0.2 968,031 - A4249_RS04655 0.31 -1.6 968,044 - A4249_RS04655 0.32 -0.8 968,082 + A4249_RS04655 0.35 -3.3 968,082 + A4249_RS04655 0.35 -2.4 968,082 + A4249_RS04655 0.35 -2.7 968,082 + A4249_RS04655 0.35 +0.2 968,083 - A4249_RS04655 0.35 -1.1 968,083 - A4249_RS04655 0.35 +0.2 968,083 - A4249_RS04655 0.35 -1.8 968,083 - A4249_RS04655 0.35 -0.8 968,084 + A4249_RS04655 0.35 -0.3 968,084 + A4249_RS04655 0.35 +0.2 968,084 + A4249_RS04655 0.35 -0.8 968,085 - A4249_RS04655 0.36 -1.4 968,085 - A4249_RS04655 0.36 -0.8 968,085 - A4249_RS04655 0.36 -0.8 968,085 - A4249_RS04655 0.36 -1.0 968,245 - A4249_RS04655 0.50 +0.7 968,532 + A4249_RS04655 0.77 +1.2 968,532 + A4249_RS04655 0.77 -1.2 968,533 - A4249_RS04655 0.77 -0.4 968,533 - A4249_RS04655 0.77 -1.1 968,533 - A4249_RS04655 0.77 -2.1 968,533 - A4249_RS04655 0.77 -2.2 968,604 + A4249_RS04655 0.84 -0.6 968,604 + A4249_RS04655 0.84 -1.2 968,604 + A4249_RS04655 0.84 -0.2 968,674 + -0.3 968,674 + -0.3 968,674 + -0.8 968,675 - -1.8 968,675 - -0.8 968,778 + -0.9 968,778 + +0.9 968,779 - -1.8 968,779 - -2.1 968,779 - +1.2 969,197 + -1.2 969,217 + +2.5
Or see this region's nucleotide sequence