Experiment: R2A_PIPES with Aluminum chloride 0.28125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pntA and cdd are separated by 135 nucleotides cdd and OKGIIK_10710 are separated by 60 nucleotides OKGIIK_10710 and OKGIIK_10715 overlap by 4 nucleotides
OKGIIK_10700: pntA - NAD(P)(+) transhydrogenase (Re/Si-specific) subunit alpha, at 2,293,057 to 2,294,157
pntA
OKGIIK_10705: cdd - cytidine deaminase, at 2,294,293 to 2,294,709
cdd
OKGIIK_10710: OKGIIK_10710 - Putative NAD(P)H nitroreductase, at 2,294,770 to 2,295,339
_10710
OKGIIK_10715: OKGIIK_10715 - Exodeoxyribonuclease IX, at 2,295,336 to 2,296,241
_10715
Position (kb)
2294
2295 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2293.485 kb on - strand, within pntA at 2293.574 kb on + strand, within pntA at 2293.575 kb on - strand, within pntA at 2294.003 kb on + strand, within pntA at 2294.003 kb on + strand, within pntA at 2294.003 kb on + strand, within pntA at 2294.004 kb on - strand, within pntA at 2294.004 kb on - strand, within pntA at 2294.004 kb on - strand, within pntA at 2294.191 kb on + strand at 2294.191 kb on + strand at 2294.233 kb on + strand at 2294.233 kb on + strand at 2294.233 kb on + strand at 2294.233 kb on + strand at 2294.233 kb on + strand at 2294.233 kb on + strand at 2294.233 kb on + strand at 2294.234 kb on - strand at 2294.234 kb on - strand at 2294.234 kb on - strand at 2294.251 kb on + strand at 2294.251 kb on + strand at 2294.252 kb on - strand at 2294.252 kb on - strand at 2294.359 kb on + strand, within cdd at 2294.359 kb on + strand, within cdd at 2294.359 kb on + strand, within cdd at 2294.359 kb on + strand, within cdd at 2294.359 kb on + strand, within cdd at 2294.359 kb on + strand, within cdd at 2294.359 kb on + strand, within cdd at 2294.360 kb on - strand, within cdd at 2294.360 kb on - strand, within cdd at 2294.360 kb on - strand, within cdd at 2294.360 kb on - strand, within cdd at 2294.368 kb on + strand, within cdd at 2294.368 kb on + strand, within cdd at 2294.369 kb on - strand, within cdd at 2294.449 kb on + strand, within cdd at 2294.449 kb on + strand, within cdd at 2294.621 kb on + strand, within cdd at 2294.621 kb on + strand, within cdd at 2294.737 kb on + strand at 2295.031 kb on + strand, within OKGIIK_10710 at 2295.031 kb on + strand, within OKGIIK_10710 at 2295.032 kb on - strand, within OKGIIK_10710 at 2295.032 kb on - strand, within OKGIIK_10710 at 2295.130 kb on + strand, within OKGIIK_10710 at 2295.130 kb on + strand, within OKGIIK_10710 at 2295.130 kb on + strand, within OKGIIK_10710 at 2295.130 kb on + strand, within OKGIIK_10710 at 2295.130 kb on + strand, within OKGIIK_10710 at 2295.131 kb on - strand, within OKGIIK_10710 at 2295.131 kb on - strand, within OKGIIK_10710 at 2295.131 kb on - strand, within OKGIIK_10710 at 2295.131 kb on - strand, within OKGIIK_10710 at 2295.131 kb on - strand, within OKGIIK_10710 at 2295.131 kb on - strand, within OKGIIK_10710 at 2295.151 kb on + strand, within OKGIIK_10710 at 2295.151 kb on + strand, within OKGIIK_10710 at 2295.151 kb on + strand, within OKGIIK_10710 at 2295.151 kb on + strand, within OKGIIK_10710 at 2295.151 kb on + strand, within OKGIIK_10710 at 2295.151 kb on + strand, within OKGIIK_10710 at 2295.151 kb on + strand, within OKGIIK_10710 at 2295.151 kb on + strand, within OKGIIK_10710 at 2295.151 kb on + strand, within OKGIIK_10710 at 2295.151 kb on + strand, within OKGIIK_10710 at 2295.151 kb on + strand, within OKGIIK_10710 at 2295.151 kb on + strand, within OKGIIK_10710 at 2295.152 kb on - strand, within OKGIIK_10710 at 2295.152 kb on - strand, within OKGIIK_10710 at 2295.152 kb on - strand, within OKGIIK_10710 at 2295.152 kb on - strand, within OKGIIK_10710 at 2295.152 kb on - strand, within OKGIIK_10710 at 2295.152 kb on - strand, within OKGIIK_10710 at 2295.152 kb on - strand, within OKGIIK_10710 at 2295.152 kb on - strand, within OKGIIK_10710 at 2295.196 kb on + strand, within OKGIIK_10710 at 2295.196 kb on + strand, within OKGIIK_10710 at 2295.196 kb on + strand, within OKGIIK_10710 at 2295.196 kb on + strand, within OKGIIK_10710 at 2295.196 kb on + strand, within OKGIIK_10710 at 2295.196 kb on + strand, within OKGIIK_10710 at 2295.390 kb on + strand at 2295.390 kb on + strand at 2295.391 kb on - strand at 2295.391 kb on - strand at 2295.391 kb on - strand at 2295.468 kb on + strand, within OKGIIK_10715 at 2295.567 kb on + strand, within OKGIIK_10715 at 2295.568 kb on - strand, within OKGIIK_10715 at 2295.568 kb on - strand, within OKGIIK_10715 at 2295.576 kb on + strand, within OKGIIK_10715 at 2295.576 kb on + strand, within OKGIIK_10715 at 2295.576 kb on + strand, within OKGIIK_10715 at 2295.577 kb on - strand, within OKGIIK_10715 at 2295.577 kb on - strand, within OKGIIK_10715 at 2295.577 kb on - strand, within OKGIIK_10715 at 2295.577 kb on - strand, within OKGIIK_10715 at 2295.707 kb on + strand, within OKGIIK_10715
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Aluminum chloride 0.28125 mM remove 2,293,485 - pntA OKGIIK_10700 0.39 -0.5 2,293,574 + pntA OKGIIK_10700 0.47 -0.1 2,293,575 - pntA OKGIIK_10700 0.47 -0.7 2,294,003 + pntA OKGIIK_10700 0.86 -0.2 2,294,003 + pntA OKGIIK_10700 0.86 -2.0 2,294,003 + pntA OKGIIK_10700 0.86 -0.2 2,294,004 - pntA OKGIIK_10700 0.86 +0.9 2,294,004 - pntA OKGIIK_10700 0.86 -0.7 2,294,004 - pntA OKGIIK_10700 0.86 +1.4 2,294,191 + +0.7 2,294,191 + +0.2 2,294,233 + -1.1 2,294,233 + +0.8 2,294,233 + +1.4 2,294,233 + +0.1 2,294,233 + -0.9 2,294,233 + +0.4 2,294,233 + +2.7 2,294,234 - +0.4 2,294,234 - -0.3 2,294,234 - +1.3 2,294,251 + +0.1 2,294,251 + -1.0 2,294,252 - -1.3 2,294,252 - +0.0 2,294,359 + cdd OKGIIK_10705 0.16 +0.1 2,294,359 + cdd OKGIIK_10705 0.16 -0.2 2,294,359 + cdd OKGIIK_10705 0.16 +0.2 2,294,359 + cdd OKGIIK_10705 0.16 -2.0 2,294,359 + cdd OKGIIK_10705 0.16 -0.8 2,294,359 + cdd OKGIIK_10705 0.16 +1.6 2,294,359 + cdd OKGIIK_10705 0.16 +0.3 2,294,360 - cdd OKGIIK_10705 0.16 -1.3 2,294,360 - cdd OKGIIK_10705 0.16 -1.5 2,294,360 - cdd OKGIIK_10705 0.16 +0.2 2,294,360 - cdd OKGIIK_10705 0.16 +0.4 2,294,368 + cdd OKGIIK_10705 0.18 -0.0 2,294,368 + cdd OKGIIK_10705 0.18 +1.2 2,294,369 - cdd OKGIIK_10705 0.18 -1.6 2,294,449 + cdd OKGIIK_10705 0.37 +1.3 2,294,449 + cdd OKGIIK_10705 0.37 +0.2 2,294,621 + cdd OKGIIK_10705 0.79 +0.1 2,294,621 + cdd OKGIIK_10705 0.79 -0.0 2,294,737 + +0.2 2,295,031 + OKGIIK_10710 0.46 -0.6 2,295,031 + OKGIIK_10710 0.46 -1.2 2,295,032 - OKGIIK_10710 0.46 -1.2 2,295,032 - OKGIIK_10710 0.46 -0.9 2,295,130 + OKGIIK_10710 0.63 -0.7 2,295,130 + OKGIIK_10710 0.63 +0.5 2,295,130 + OKGIIK_10710 0.63 +0.4 2,295,130 + OKGIIK_10710 0.63 +0.7 2,295,130 + OKGIIK_10710 0.63 -1.6 2,295,131 - OKGIIK_10710 0.63 -0.3 2,295,131 - OKGIIK_10710 0.63 +0.3 2,295,131 - OKGIIK_10710 0.63 -1.7 2,295,131 - OKGIIK_10710 0.63 -0.3 2,295,131 - OKGIIK_10710 0.63 -1.7 2,295,131 - OKGIIK_10710 0.63 -0.7 2,295,151 + OKGIIK_10710 0.67 -1.0 2,295,151 + OKGIIK_10710 0.67 +0.8 2,295,151 + OKGIIK_10710 0.67 -0.5 2,295,151 + OKGIIK_10710 0.67 -0.5 2,295,151 + OKGIIK_10710 0.67 -0.3 2,295,151 + OKGIIK_10710 0.67 -0.2 2,295,151 + OKGIIK_10710 0.67 -0.8 2,295,151 + OKGIIK_10710 0.67 -0.5 2,295,151 + OKGIIK_10710 0.67 -2.7 2,295,151 + OKGIIK_10710 0.67 -2.0 2,295,151 + OKGIIK_10710 0.67 -0.9 2,295,151 + OKGIIK_10710 0.67 +0.5 2,295,152 - OKGIIK_10710 0.67 -1.5 2,295,152 - OKGIIK_10710 0.67 -0.7 2,295,152 - OKGIIK_10710 0.67 -2.7 2,295,152 - OKGIIK_10710 0.67 +1.1 2,295,152 - OKGIIK_10710 0.67 -0.2 2,295,152 - OKGIIK_10710 0.67 +1.1 2,295,152 - OKGIIK_10710 0.67 +0.9 2,295,152 - OKGIIK_10710 0.67 +2.7 2,295,196 + OKGIIK_10710 0.75 +0.0 2,295,196 + OKGIIK_10710 0.75 +1.0 2,295,196 + OKGIIK_10710 0.75 -0.0 2,295,196 + OKGIIK_10710 0.75 -0.6 2,295,196 + OKGIIK_10710 0.75 +0.4 2,295,196 + OKGIIK_10710 0.75 +1.3 2,295,390 + -1.5 2,295,390 + +0.4 2,295,391 - -1.0 2,295,391 - -1.9 2,295,391 - +0.0 2,295,468 + OKGIIK_10715 0.15 -0.7 2,295,567 + OKGIIK_10715 0.25 -0.4 2,295,568 - OKGIIK_10715 0.26 -1.4 2,295,568 - OKGIIK_10715 0.26 -1.9 2,295,576 + OKGIIK_10715 0.26 +1.0 2,295,576 + OKGIIK_10715 0.26 -1.5 2,295,576 + OKGIIK_10715 0.26 +0.6 2,295,577 - OKGIIK_10715 0.27 -0.5 2,295,577 - OKGIIK_10715 0.27 -1.0 2,295,577 - OKGIIK_10715 0.27 -1.2 2,295,577 - OKGIIK_10715 0.27 +1.2 2,295,707 + OKGIIK_10715 0.41 -0.4
Or see this region's nucleotide sequence