Experiment: R2A_PIPES with Aluminum chloride 0.28125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt leuB and OKGIIK_03815 are separated by 276 nucleotides OKGIIK_03815 and OKGIIK_03820 are separated by 36 nucleotides OKGIIK_03820 and OKGIIK_03825 are separated by 58 nucleotides
OKGIIK_03810: leuB - isocitrate dehydrogenase, at 840,498 to 841,511
leuB
OKGIIK_03815: OKGIIK_03815 - Carboxymuconolactone decarboxylase, at 841,788 to 842,249
_03815
OKGIIK_03820: OKGIIK_03820 - RNA polymerase sigma-70 factor, ECF subfamily, at 842,286 to 843,185
_03820
OKGIIK_03825: OKGIIK_03825 - Aspartyl beta-hydroxylase, at 843,244 to 844,200
_03825
Position (kb) 841
842
843 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 841.512 kb on + strand at 841.512 kb on + strand at 841.512 kb on + strand at 841.513 kb on - strand at 841.513 kb on - strand at 841.513 kb on - strand at 841.513 kb on - strand at 841.513 kb on - strand at 841.513 kb on - strand at 841.513 kb on - strand at 841.513 kb on - strand at 841.513 kb on - strand at 841.513 kb on - strand at 841.513 kb on - strand at 841.513 kb on - strand at 841.629 kb on + strand at 841.629 kb on + strand at 841.629 kb on + strand at 841.629 kb on + strand at 841.760 kb on + strand at 841.760 kb on + strand at 841.760 kb on + strand at 841.760 kb on + strand at 841.760 kb on + strand at 841.761 kb on - strand at 841.761 kb on - strand at 841.761 kb on - strand at 841.777 kb on + strand at 841.810 kb on + strand at 841.810 kb on + strand at 841.810 kb on + strand at 841.810 kb on + strand at 841.810 kb on + strand at 841.810 kb on + strand at 841.810 kb on + strand at 841.810 kb on + strand at 841.810 kb on + strand at 841.811 kb on - strand at 841.811 kb on - strand at 841.811 kb on - strand at 841.811 kb on - strand at 841.811 kb on - strand at 841.811 kb on - strand at 841.811 kb on - strand at 841.811 kb on - strand at 841.811 kb on - strand at 841.927 kb on + strand, within OKGIIK_03815 at 841.928 kb on - strand, within OKGIIK_03815 at 842.011 kb on + strand, within OKGIIK_03815 at 842.011 kb on + strand, within OKGIIK_03815 at 842.011 kb on + strand, within OKGIIK_03815 at 842.011 kb on + strand, within OKGIIK_03815 at 842.012 kb on - strand, within OKGIIK_03815 at 842.012 kb on - strand, within OKGIIK_03815 at 842.012 kb on - strand, within OKGIIK_03815 at 842.012 kb on - strand, within OKGIIK_03815 at 842.012 kb on - strand, within OKGIIK_03815 at 842.012 kb on - strand, within OKGIIK_03815 at 842.012 kb on - strand, within OKGIIK_03815 at 842.104 kb on + strand, within OKGIIK_03815 at 842.104 kb on + strand, within OKGIIK_03815 at 842.104 kb on + strand, within OKGIIK_03815 at 842.104 kb on + strand, within OKGIIK_03815 at 842.104 kb on + strand, within OKGIIK_03815 at 842.105 kb on - strand, within OKGIIK_03815 at 842.221 kb on + strand at 842.221 kb on + strand at 842.221 kb on + strand at 842.221 kb on + strand at 842.222 kb on - strand at 842.222 kb on - strand at 842.249 kb on + strand at 842.249 kb on + strand at 842.249 kb on + strand at 842.249 kb on + strand at 842.249 kb on + strand at 842.249 kb on + strand at 842.249 kb on + strand at 842.249 kb on + strand at 842.249 kb on + strand at 842.249 kb on + strand at 842.249 kb on + strand at 842.249 kb on + strand at 842.249 kb on + strand at 842.249 kb on + strand at 842.249 kb on + strand at 842.249 kb on + strand at 842.249 kb on + strand at 842.249 kb on + strand at 842.250 kb on - strand at 842.250 kb on - strand at 842.250 kb on - strand at 842.250 kb on - strand at 842.250 kb on - strand at 842.250 kb on - strand at 842.250 kb on - strand at 842.250 kb on - strand at 842.250 kb on - strand at 842.250 kb on - strand at 842.470 kb on + strand, within OKGIIK_03820 at 842.470 kb on + strand, within OKGIIK_03820 at 842.482 kb on + strand, within OKGIIK_03820 at 842.482 kb on + strand, within OKGIIK_03820 at 842.482 kb on + strand, within OKGIIK_03820 at 842.482 kb on + strand, within OKGIIK_03820 at 842.483 kb on - strand, within OKGIIK_03820 at 842.483 kb on - strand, within OKGIIK_03820 at 842.483 kb on - strand, within OKGIIK_03820 at 842.483 kb on - strand, within OKGIIK_03820 at 842.483 kb on - strand, within OKGIIK_03820 at 842.483 kb on - strand, within OKGIIK_03820 at 842.483 kb on - strand, within OKGIIK_03820 at 842.491 kb on - strand, within OKGIIK_03820 at 842.492 kb on + strand, within OKGIIK_03820 at 842.492 kb on + strand, within OKGIIK_03820 at 842.492 kb on + strand, within OKGIIK_03820 at 842.492 kb on + strand, within OKGIIK_03820 at 842.492 kb on + strand, within OKGIIK_03820 at 842.492 kb on + strand, within OKGIIK_03820 at 842.493 kb on - strand, within OKGIIK_03820 at 842.493 kb on - strand, within OKGIIK_03820 at 842.513 kb on - strand, within OKGIIK_03820 at 842.513 kb on - strand, within OKGIIK_03820 at 842.540 kb on - strand, within OKGIIK_03820 at 842.540 kb on - strand, within OKGIIK_03820 at 842.541 kb on + strand, within OKGIIK_03820 at 842.541 kb on + strand, within OKGIIK_03820 at 842.542 kb on - strand, within OKGIIK_03820 at 842.797 kb on + strand, within OKGIIK_03820 at 842.797 kb on + strand, within OKGIIK_03820 at 842.797 kb on + strand, within OKGIIK_03820 at 842.797 kb on + strand, within OKGIIK_03820 at 842.798 kb on - strand, within OKGIIK_03820 at 842.798 kb on - strand, within OKGIIK_03820 at 842.798 kb on - strand, within OKGIIK_03820 at 842.805 kb on + strand, within OKGIIK_03820 at 842.805 kb on + strand, within OKGIIK_03820 at 842.805 kb on + strand, within OKGIIK_03820 at 842.805 kb on + strand, within OKGIIK_03820 at 842.805 kb on + strand, within OKGIIK_03820 at 842.805 kb on + strand, within OKGIIK_03820 at 842.805 kb on + strand, within OKGIIK_03820 at 842.806 kb on - strand, within OKGIIK_03820 at 842.806 kb on - strand, within OKGIIK_03820 at 842.806 kb on - strand, within OKGIIK_03820 at 842.806 kb on - strand, within OKGIIK_03820 at 842.806 kb on - strand, within OKGIIK_03820 at 842.876 kb on + strand, within OKGIIK_03820 at 842.876 kb on + strand, within OKGIIK_03820 at 842.876 kb on + strand, within OKGIIK_03820 at 842.877 kb on - strand, within OKGIIK_03820 at 842.877 kb on - strand, within OKGIIK_03820 at 842.877 kb on - strand, within OKGIIK_03820 at 842.877 kb on - strand, within OKGIIK_03820 at 842.877 kb on - strand, within OKGIIK_03820 at 842.957 kb on - strand, within OKGIIK_03820 at 842.957 kb on - strand, within OKGIIK_03820 at 843.127 kb on + strand at 843.127 kb on + strand at 843.128 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Aluminum chloride 0.28125 mM remove 841,512 + +0.2 841,512 + -0.4 841,512 + -0.6 841,513 - -0.6 841,513 - -0.1 841,513 - -0.7 841,513 - +0.4 841,513 - +0.4 841,513 - -0.7 841,513 - -0.7 841,513 - -0.0 841,513 - +0.7 841,513 - -0.9 841,513 - -0.2 841,513 - -0.3 841,629 + -0.6 841,629 + -0.9 841,629 + +0.3 841,629 + -0.7 841,760 + +0.9 841,760 + +1.0 841,760 + +0.3 841,760 + -0.1 841,760 + -1.3 841,761 - -0.8 841,761 - +0.1 841,761 - -0.3 841,777 + +1.7 841,810 + +1.6 841,810 + -0.9 841,810 + -1.2 841,810 + +0.1 841,810 + -1.4 841,810 + -0.1 841,810 + -1.4 841,810 + -1.6 841,810 + -1.4 841,811 - -2.5 841,811 - +0.1 841,811 - -1.7 841,811 - -3.4 841,811 - +0.6 841,811 - +0.7 841,811 - -0.7 841,811 - -0.0 841,811 - -2.2 841,927 + OKGIIK_03815 0.30 +1.6 841,928 - OKGIIK_03815 0.30 -0.3 842,011 + OKGIIK_03815 0.48 -1.6 842,011 + OKGIIK_03815 0.48 -0.6 842,011 + OKGIIK_03815 0.48 -1.3 842,011 + OKGIIK_03815 0.48 +2.1 842,012 - OKGIIK_03815 0.48 -1.0 842,012 - OKGIIK_03815 0.48 -0.3 842,012 - OKGIIK_03815 0.48 -1.1 842,012 - OKGIIK_03815 0.48 -0.9 842,012 - OKGIIK_03815 0.48 -0.4 842,012 - OKGIIK_03815 0.48 -0.5 842,012 - OKGIIK_03815 0.48 +0.0 842,104 + OKGIIK_03815 0.68 -0.4 842,104 + OKGIIK_03815 0.68 -0.1 842,104 + OKGIIK_03815 0.68 +0.4 842,104 + OKGIIK_03815 0.68 -0.0 842,104 + OKGIIK_03815 0.68 +0.9 842,105 - OKGIIK_03815 0.69 -1.9 842,221 + -0.9 842,221 + -0.1 842,221 + -0.3 842,221 + -0.5 842,222 - -0.8 842,222 - -1.1 842,249 + -2.0 842,249 + -0.8 842,249 + -1.1 842,249 + +0.1 842,249 + +0.9 842,249 + +1.2 842,249 + +1.3 842,249 + +0.7 842,249 + -0.7 842,249 + +1.9 842,249 + +0.2 842,249 + +1.6 842,249 + +0.0 842,249 + -0.1 842,249 + +1.0 842,249 + +0.3 842,249 + -0.7 842,249 + -0.1 842,250 - +1.0 842,250 - +0.9 842,250 - +1.1 842,250 - -0.6 842,250 - +1.3 842,250 - -0.4 842,250 - +1.2 842,250 - +0.3 842,250 - -0.9 842,250 - -0.6 842,470 + OKGIIK_03820 0.20 +0.5 842,470 + OKGIIK_03820 0.20 -1.9 842,482 + OKGIIK_03820 0.22 -1.1 842,482 + OKGIIK_03820 0.22 -0.2 842,482 + OKGIIK_03820 0.22 -0.1 842,482 + OKGIIK_03820 0.22 -0.1 842,483 - OKGIIK_03820 0.22 -1.1 842,483 - OKGIIK_03820 0.22 +1.3 842,483 - OKGIIK_03820 0.22 +0.4 842,483 - OKGIIK_03820 0.22 -1.3 842,483 - OKGIIK_03820 0.22 -1.3 842,483 - OKGIIK_03820 0.22 +0.1 842,483 - OKGIIK_03820 0.22 -4.2 842,491 - OKGIIK_03820 0.23 +0.3 842,492 + OKGIIK_03820 0.23 +0.6 842,492 + OKGIIK_03820 0.23 -0.3 842,492 + OKGIIK_03820 0.23 -0.4 842,492 + OKGIIK_03820 0.23 -1.3 842,492 + OKGIIK_03820 0.23 -0.4 842,492 + OKGIIK_03820 0.23 +0.7 842,493 - OKGIIK_03820 0.23 -0.7 842,493 - OKGIIK_03820 0.23 -0.2 842,513 - OKGIIK_03820 0.25 -0.7 842,513 - OKGIIK_03820 0.25 +0.0 842,540 - OKGIIK_03820 0.28 -0.7 842,540 - OKGIIK_03820 0.28 -0.4 842,541 + OKGIIK_03820 0.28 -0.4 842,541 + OKGIIK_03820 0.28 +1.0 842,542 - OKGIIK_03820 0.28 -0.2 842,797 + OKGIIK_03820 0.57 +0.2 842,797 + OKGIIK_03820 0.57 -1.6 842,797 + OKGIIK_03820 0.57 -0.9 842,797 + OKGIIK_03820 0.57 -0.4 842,798 - OKGIIK_03820 0.57 +0.2 842,798 - OKGIIK_03820 0.57 -0.2 842,798 - OKGIIK_03820 0.57 -1.1 842,805 + OKGIIK_03820 0.58 -1.9 842,805 + OKGIIK_03820 0.58 -0.7 842,805 + OKGIIK_03820 0.58 +0.8 842,805 + OKGIIK_03820 0.58 -0.0 842,805 + OKGIIK_03820 0.58 -1.0 842,805 + OKGIIK_03820 0.58 +0.7 842,805 + OKGIIK_03820 0.58 +0.1 842,806 - OKGIIK_03820 0.58 -0.4 842,806 - OKGIIK_03820 0.58 -0.8 842,806 - OKGIIK_03820 0.58 -0.9 842,806 - OKGIIK_03820 0.58 -0.7 842,806 - OKGIIK_03820 0.58 +1.4 842,876 + OKGIIK_03820 0.66 +1.1 842,876 + OKGIIK_03820 0.66 -1.3 842,876 + OKGIIK_03820 0.66 -1.7 842,877 - OKGIIK_03820 0.66 +0.6 842,877 - OKGIIK_03820 0.66 +0.1 842,877 - OKGIIK_03820 0.66 -1.2 842,877 - OKGIIK_03820 0.66 -0.2 842,877 - OKGIIK_03820 0.66 +0.7 842,957 - OKGIIK_03820 0.75 +0.1 842,957 - OKGIIK_03820 0.75 +0.9 843,127 + +2.5 843,127 + +0.9 843,128 - +1.5
Or see this region's nucleotide sequence