Experiment: R2A_PIPES with Aluminum chloride 0.28125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fixX and gatA are separated by 162 nucleotides gatA and cysD are separated by 214 nucleotides
OKGIIK_01285: fixX - electron transfer flavoprotein-ubiquinone oxidoreductase, at 282,351 to 283,964
fixX
OKGIIK_01290: gatA - amidase, at 284,127 to 285,524
gatA
OKGIIK_01295: cysD - sulfate adenylyltransferase subunit CysD, at 285,739 to 286,656
cysD
Position (kb) 284
285
286 Strain fitness (log2 ratio) -2
-1
0
1
2 at 283.165 kb on + strand, within fixX at 283.165 kb on + strand, within fixX at 283.166 kb on - strand, within fixX at 283.198 kb on + strand, within fixX at 283.231 kb on + strand, within fixX at 283.231 kb on + strand, within fixX at 283.231 kb on + strand, within fixX at 283.232 kb on - strand, within fixX at 283.232 kb on - strand, within fixX at 283.232 kb on - strand, within fixX at 283.232 kb on - strand, within fixX at 283.232 kb on - strand, within fixX at 283.249 kb on + strand, within fixX at 283.250 kb on - strand, within fixX at 283.250 kb on - strand, within fixX at 283.253 kb on + strand, within fixX at 283.253 kb on + strand, within fixX at 283.253 kb on + strand, within fixX at 283.253 kb on + strand, within fixX at 283.253 kb on + strand, within fixX at 283.253 kb on + strand, within fixX at 283.253 kb on + strand, within fixX at 283.253 kb on + strand, within fixX at 283.253 kb on + strand, within fixX at 283.253 kb on + strand, within fixX at 283.253 kb on + strand, within fixX at 283.253 kb on + strand, within fixX at 283.253 kb on + strand, within fixX at 283.253 kb on + strand, within fixX at 283.253 kb on + strand, within fixX at 283.254 kb on - strand, within fixX at 283.254 kb on - strand, within fixX at 283.254 kb on - strand, within fixX at 283.254 kb on - strand, within fixX at 283.254 kb on - strand, within fixX at 283.254 kb on - strand, within fixX at 283.254 kb on - strand, within fixX at 283.254 kb on - strand, within fixX at 283.254 kb on - strand, within fixX at 283.254 kb on - strand, within fixX at 283.254 kb on - strand, within fixX at 283.254 kb on - strand, within fixX at 283.382 kb on - strand, within fixX at 283.469 kb on - strand, within fixX at 283.469 kb on - strand, within fixX at 283.546 kb on + strand, within fixX at 283.546 kb on + strand, within fixX at 283.546 kb on + strand, within fixX at 283.547 kb on - strand, within fixX at 283.547 kb on - strand, within fixX at 283.547 kb on - strand, within fixX at 283.547 kb on - strand, within fixX at 283.547 kb on - strand, within fixX at 283.547 kb on - strand, within fixX at 283.939 kb on + strand at 283.939 kb on + strand at 283.940 kb on - strand at 284.010 kb on + strand at 284.010 kb on + strand at 284.011 kb on - strand at 284.238 kb on + strand at 284.238 kb on + strand at 284.239 kb on - strand at 284.239 kb on - strand at 284.239 kb on - strand at 284.239 kb on - strand at 284.239 kb on - strand at 284.239 kb on - strand at 284.239 kb on - strand at 284.239 kb on - strand at 284.280 kb on + strand, within gatA at 284.280 kb on + strand, within gatA at 284.280 kb on + strand, within gatA at 284.280 kb on + strand, within gatA at 284.280 kb on + strand, within gatA at 284.280 kb on + strand, within gatA at 284.280 kb on + strand, within gatA at 284.280 kb on + strand, within gatA at 284.280 kb on + strand, within gatA at 284.280 kb on + strand, within gatA at 284.280 kb on + strand, within gatA at 284.281 kb on - strand, within gatA at 284.281 kb on - strand, within gatA at 284.281 kb on - strand, within gatA at 284.281 kb on - strand, within gatA at 284.281 kb on - strand, within gatA at 284.281 kb on - strand, within gatA at 284.281 kb on - strand, within gatA at 284.281 kb on - strand, within gatA at 284.281 kb on - strand, within gatA at 284.281 kb on - strand, within gatA at 284.700 kb on + strand, within gatA at 284.700 kb on + strand, within gatA at 284.700 kb on + strand, within gatA at 284.700 kb on + strand, within gatA at 284.700 kb on + strand, within gatA at 284.700 kb on + strand, within gatA at 284.701 kb on - strand, within gatA at 284.701 kb on - strand, within gatA at 284.701 kb on - strand, within gatA at 284.701 kb on - strand, within gatA at 284.701 kb on - strand, within gatA at 284.712 kb on + strand, within gatA at 284.712 kb on + strand, within gatA at 284.712 kb on + strand, within gatA at 284.712 kb on + strand, within gatA at 284.713 kb on - strand, within gatA at 285.198 kb on + strand, within gatA at 285.198 kb on + strand, within gatA at 285.199 kb on - strand, within gatA at 285.507 kb on + strand at 285.507 kb on + strand at 285.507 kb on + strand at 285.508 kb on - strand at 285.508 kb on - strand at 285.508 kb on - strand at 285.615 kb on + strand at 285.615 kb on + strand at 285.615 kb on + strand at 285.615 kb on + strand at 285.615 kb on + strand at 285.615 kb on + strand at 285.615 kb on + strand at 285.615 kb on + strand at 285.615 kb on + strand at 285.616 kb on - strand at 285.616 kb on - strand at 285.616 kb on - strand at 285.616 kb on - strand at 285.649 kb on + strand at 285.649 kb on + strand at 285.649 kb on + strand at 285.650 kb on - strand at 285.676 kb on + strand at 285.676 kb on + strand at 285.676 kb on + strand at 285.676 kb on + strand at 285.677 kb on - strand at 285.677 kb on - strand at 285.744 kb on + strand at 285.744 kb on + strand at 285.744 kb on + strand at 285.744 kb on + strand at 285.744 kb on + strand at 285.744 kb on + strand at 285.744 kb on + strand at 285.745 kb on - strand at 285.745 kb on - strand at 285.745 kb on - strand at 285.745 kb on - strand at 285.847 kb on + strand, within cysD at 285.847 kb on + strand, within cysD at 285.847 kb on + strand, within cysD at 285.847 kb on + strand, within cysD at 285.847 kb on + strand, within cysD at 285.848 kb on - strand, within cysD at 285.848 kb on - strand, within cysD at 285.848 kb on - strand, within cysD at 285.848 kb on - strand, within cysD at 285.848 kb on - strand, within cysD at 285.848 kb on - strand, within cysD at 285.848 kb on - strand, within cysD at 285.902 kb on - strand, within cysD at 286.077 kb on + strand, within cysD at 286.077 kb on + strand, within cysD at 286.078 kb on - strand, within cysD at 286.078 kb on - strand, within cysD at 286.078 kb on - strand, within cysD at 286.336 kb on + strand, within cysD at 286.336 kb on + strand, within cysD at 286.336 kb on + strand, within cysD at 286.336 kb on + strand, within cysD at 286.336 kb on + strand, within cysD at 286.336 kb on + strand, within cysD at 286.336 kb on + strand, within cysD at 286.336 kb on + strand, within cysD at 286.336 kb on + strand, within cysD at 286.336 kb on + strand, within cysD at 286.337 kb on - strand, within cysD at 286.337 kb on - strand, within cysD at 286.337 kb on - strand, within cysD at 286.337 kb on - strand, within cysD at 286.337 kb on - strand, within cysD at 286.337 kb on - strand, within cysD at 286.337 kb on - strand, within cysD at 286.337 kb on - strand, within cysD at 286.337 kb on - strand, within cysD at 286.337 kb on - strand, within cysD at 286.337 kb on - strand, within cysD at 286.337 kb on - strand, within cysD at 286.337 kb on - strand, within cysD at 286.337 kb on - strand, within cysD at 286.337 kb on - strand, within cysD at 286.337 kb on - strand, within cysD at 286.372 kb on + strand, within cysD at 286.372 kb on + strand, within cysD at 286.372 kb on + strand, within cysD at 286.372 kb on + strand, within cysD at 286.372 kb on + strand, within cysD at 286.372 kb on + strand, within cysD at 286.373 kb on - strand, within cysD at 286.373 kb on - strand, within cysD at 286.373 kb on - strand, within cysD at 286.373 kb on - strand, within cysD at 286.373 kb on - strand, within cysD at 286.501 kb on + strand, within cysD
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Aluminum chloride 0.28125 mM remove 283,165 + fixX OKGIIK_01285 0.50 +1.5 283,165 + fixX OKGIIK_01285 0.50 -0.2 283,166 - fixX OKGIIK_01285 0.50 +1.4 283,198 + fixX OKGIIK_01285 0.52 -0.2 283,231 + fixX OKGIIK_01285 0.55 +1.5 283,231 + fixX OKGIIK_01285 0.55 -0.5 283,231 + fixX OKGIIK_01285 0.55 -1.7 283,232 - fixX OKGIIK_01285 0.55 -1.5 283,232 - fixX OKGIIK_01285 0.55 -0.5 283,232 - fixX OKGIIK_01285 0.55 -0.6 283,232 - fixX OKGIIK_01285 0.55 -1.2 283,232 - fixX OKGIIK_01285 0.55 -0.8 283,249 + fixX OKGIIK_01285 0.56 +0.1 283,250 - fixX OKGIIK_01285 0.56 -0.5 283,250 - fixX OKGIIK_01285 0.56 +0.8 283,253 + fixX OKGIIK_01285 0.56 +0.2 283,253 + fixX OKGIIK_01285 0.56 +0.0 283,253 + fixX OKGIIK_01285 0.56 -1.5 283,253 + fixX OKGIIK_01285 0.56 +1.0 283,253 + fixX OKGIIK_01285 0.56 -0.5 283,253 + fixX OKGIIK_01285 0.56 -0.4 283,253 + fixX OKGIIK_01285 0.56 +1.5 283,253 + fixX OKGIIK_01285 0.56 -1.4 283,253 + fixX OKGIIK_01285 0.56 -0.1 283,253 + fixX OKGIIK_01285 0.56 -0.4 283,253 + fixX OKGIIK_01285 0.56 -1.5 283,253 + fixX OKGIIK_01285 0.56 -0.5 283,253 + fixX OKGIIK_01285 0.56 -0.4 283,253 + fixX OKGIIK_01285 0.56 +0.6 283,253 + fixX OKGIIK_01285 0.56 -0.4 283,254 - fixX OKGIIK_01285 0.56 -1.4 283,254 - fixX OKGIIK_01285 0.56 +0.3 283,254 - fixX OKGIIK_01285 0.56 -0.1 283,254 - fixX OKGIIK_01285 0.56 -0.5 283,254 - fixX OKGIIK_01285 0.56 -0.1 283,254 - fixX OKGIIK_01285 0.56 -1.1 283,254 - fixX OKGIIK_01285 0.56 -0.2 283,254 - fixX OKGIIK_01285 0.56 -2.0 283,254 - fixX OKGIIK_01285 0.56 +0.4 283,254 - fixX OKGIIK_01285 0.56 +0.5 283,254 - fixX OKGIIK_01285 0.56 -1.1 283,254 - fixX OKGIIK_01285 0.56 +0.8 283,382 - fixX OKGIIK_01285 0.64 -0.5 283,469 - fixX OKGIIK_01285 0.69 +0.2 283,469 - fixX OKGIIK_01285 0.69 -1.0 283,546 + fixX OKGIIK_01285 0.74 -0.8 283,546 + fixX OKGIIK_01285 0.74 +1.4 283,546 + fixX OKGIIK_01285 0.74 +0.7 283,547 - fixX OKGIIK_01285 0.74 -1.7 283,547 - fixX OKGIIK_01285 0.74 -1.8 283,547 - fixX OKGIIK_01285 0.74 -2.0 283,547 - fixX OKGIIK_01285 0.74 +0.5 283,547 - fixX OKGIIK_01285 0.74 -0.4 283,547 - fixX OKGIIK_01285 0.74 -0.8 283,939 + -1.0 283,939 + -1.2 283,940 - -0.2 284,010 + +1.0 284,010 + -0.9 284,011 - -0.2 284,238 + -0.6 284,238 + +0.3 284,239 - -1.3 284,239 - +1.0 284,239 - -0.8 284,239 - -0.7 284,239 - -0.2 284,239 - +1.5 284,239 - -0.7 284,239 - -0.3 284,280 + gatA OKGIIK_01290 0.11 +0.7 284,280 + gatA OKGIIK_01290 0.11 -0.8 284,280 + gatA OKGIIK_01290 0.11 +1.0 284,280 + gatA OKGIIK_01290 0.11 -1.0 284,280 + gatA OKGIIK_01290 0.11 +0.8 284,280 + gatA OKGIIK_01290 0.11 +0.2 284,280 + gatA OKGIIK_01290 0.11 +1.2 284,280 + gatA OKGIIK_01290 0.11 -0.5 284,280 + gatA OKGIIK_01290 0.11 +1.3 284,280 + gatA OKGIIK_01290 0.11 +0.4 284,280 + gatA OKGIIK_01290 0.11 -0.8 284,281 - gatA OKGIIK_01290 0.11 -0.5 284,281 - gatA OKGIIK_01290 0.11 -0.0 284,281 - gatA OKGIIK_01290 0.11 -2.5 284,281 - gatA OKGIIK_01290 0.11 +1.6 284,281 - gatA OKGIIK_01290 0.11 +1.5 284,281 - gatA OKGIIK_01290 0.11 +0.8 284,281 - gatA OKGIIK_01290 0.11 +0.2 284,281 - gatA OKGIIK_01290 0.11 -2.0 284,281 - gatA OKGIIK_01290 0.11 -1.0 284,281 - gatA OKGIIK_01290 0.11 -2.2 284,700 + gatA OKGIIK_01290 0.41 -0.5 284,700 + gatA OKGIIK_01290 0.41 -0.5 284,700 + gatA OKGIIK_01290 0.41 -0.6 284,700 + gatA OKGIIK_01290 0.41 +0.1 284,700 + gatA OKGIIK_01290 0.41 +0.9 284,700 + gatA OKGIIK_01290 0.41 -1.5 284,701 - gatA OKGIIK_01290 0.41 +0.8 284,701 - gatA OKGIIK_01290 0.41 -1.2 284,701 - gatA OKGIIK_01290 0.41 -1.3 284,701 - gatA OKGIIK_01290 0.41 +0.1 284,701 - gatA OKGIIK_01290 0.41 -0.2 284,712 + gatA OKGIIK_01290 0.42 -0.1 284,712 + gatA OKGIIK_01290 0.42 +0.7 284,712 + gatA OKGIIK_01290 0.42 -1.5 284,712 + gatA OKGIIK_01290 0.42 -1.9 284,713 - gatA OKGIIK_01290 0.42 -0.8 285,198 + gatA OKGIIK_01290 0.77 +0.2 285,198 + gatA OKGIIK_01290 0.77 -1.3 285,199 - gatA OKGIIK_01290 0.77 -2.1 285,507 + -0.9 285,507 + -0.1 285,507 + -0.8 285,508 - -0.2 285,508 - -2.4 285,508 - -0.3 285,615 + +0.6 285,615 + +1.4 285,615 + -0.5 285,615 + +0.8 285,615 + -0.1 285,615 + +0.1 285,615 + -1.4 285,615 + -0.1 285,615 + +0.3 285,616 - -2.7 285,616 - +1.5 285,616 - +0.2 285,616 - -1.7 285,649 + -0.6 285,649 + -0.0 285,649 + +0.2 285,650 - -0.2 285,676 + -0.9 285,676 + +0.7 285,676 + -1.5 285,676 + +1.3 285,677 - +0.3 285,677 - -1.0 285,744 + -1.2 285,744 + +0.8 285,744 + +0.5 285,744 + +0.2 285,744 + +1.8 285,744 + +0.3 285,744 + +1.2 285,745 - +0.4 285,745 - +0.2 285,745 - -0.2 285,745 - +0.3 285,847 + cysD OKGIIK_01295 0.12 -1.1 285,847 + cysD OKGIIK_01295 0.12 -0.2 285,847 + cysD OKGIIK_01295 0.12 -0.5 285,847 + cysD OKGIIK_01295 0.12 -0.4 285,847 + cysD OKGIIK_01295 0.12 -1.2 285,848 - cysD OKGIIK_01295 0.12 -0.5 285,848 - cysD OKGIIK_01295 0.12 +0.2 285,848 - cysD OKGIIK_01295 0.12 -0.5 285,848 - cysD OKGIIK_01295 0.12 +0.2 285,848 - cysD OKGIIK_01295 0.12 +0.9 285,848 - cysD OKGIIK_01295 0.12 -0.5 285,848 - cysD OKGIIK_01295 0.12 -0.2 285,902 - cysD OKGIIK_01295 0.18 -1.1 286,077 + cysD OKGIIK_01295 0.37 -2.1 286,077 + cysD OKGIIK_01295 0.37 -0.0 286,078 - cysD OKGIIK_01295 0.37 -0.3 286,078 - cysD OKGIIK_01295 0.37 -0.8 286,078 - cysD OKGIIK_01295 0.37 -0.5 286,336 + cysD OKGIIK_01295 0.65 +0.6 286,336 + cysD OKGIIK_01295 0.65 -0.7 286,336 + cysD OKGIIK_01295 0.65 +1.0 286,336 + cysD OKGIIK_01295 0.65 -0.4 286,336 + cysD OKGIIK_01295 0.65 -1.0 286,336 + cysD OKGIIK_01295 0.65 -0.3 286,336 + cysD OKGIIK_01295 0.65 -0.8 286,336 + cysD OKGIIK_01295 0.65 +1.2 286,336 + cysD OKGIIK_01295 0.65 +0.1 286,336 + cysD OKGIIK_01295 0.65 -0.4 286,337 - cysD OKGIIK_01295 0.65 +1.5 286,337 - cysD OKGIIK_01295 0.65 -1.3 286,337 - cysD OKGIIK_01295 0.65 +0.0 286,337 - cysD OKGIIK_01295 0.65 +0.2 286,337 - cysD OKGIIK_01295 0.65 -1.0 286,337 - cysD OKGIIK_01295 0.65 -2.1 286,337 - cysD OKGIIK_01295 0.65 -0.6 286,337 - cysD OKGIIK_01295 0.65 -0.5 286,337 - cysD OKGIIK_01295 0.65 -1.0 286,337 - cysD OKGIIK_01295 0.65 -0.0 286,337 - cysD OKGIIK_01295 0.65 -1.3 286,337 - cysD OKGIIK_01295 0.65 +1.0 286,337 - cysD OKGIIK_01295 0.65 -0.2 286,337 - cysD OKGIIK_01295 0.65 -1.2 286,337 - cysD OKGIIK_01295 0.65 -0.4 286,337 - cysD OKGIIK_01295 0.65 -1.3 286,372 + cysD OKGIIK_01295 0.69 -0.7 286,372 + cysD OKGIIK_01295 0.69 +1.8 286,372 + cysD OKGIIK_01295 0.69 -1.0 286,372 + cysD OKGIIK_01295 0.69 +1.7 286,372 + cysD OKGIIK_01295 0.69 -1.4 286,372 + cysD OKGIIK_01295 0.69 -0.7 286,373 - cysD OKGIIK_01295 0.69 -0.5 286,373 - cysD OKGIIK_01295 0.69 -0.6 286,373 - cysD OKGIIK_01295 0.69 -0.1 286,373 - cysD OKGIIK_01295 0.69 -1.4 286,373 - cysD OKGIIK_01295 0.69 +1.8 286,501 + cysD OKGIIK_01295 0.83 -0.1
Or see this region's nucleotide sequence