Experiment: no stress control with NaOH
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt QF045_RS06595 and QF045_RS06600 overlap by 8 nucleotides QF045_RS06600 and QF045_RS06605 are separated by 99 nucleotides QF045_RS06605 and QF045_RS06610 are separated by 66 nucleotides QF045_RS06610 and QF045_RS06615 are separated by 54 nucleotides QF045_RS06615 and QF045_RS06620 overlap by 8 nucleotides
QF045_RS06595: QF045_RS06595 - molybdopterin-synthase adenylyltransferase MoeB, at 1,484,958 to 1,485,714
_RS06595
QF045_RS06600: QF045_RS06600 - glutamate racemase, at 1,485,707 to 1,486,501
_RS06600
QF045_RS06605: QF045_RS06605 - acyloxyacyl hydrolase, at 1,486,601 to 1,487,119
_RS06605
QF045_RS06610: QF045_RS06610 - YkgJ family cysteine cluster protein, at 1,487,186 to 1,487,491
_RS06610
QF045_RS06615: QF045_RS06615 - DUF2878 domain-containing protein, at 1,487,546 to 1,488,055
_RS06615
QF045_RS06620: QF045_RS06620 - cyclopropane-fatty-acyl-phospholipid synthase family protein, at 1,488,048 to 1,489,319
_RS06620
Position (kb)
1486
1487
1488 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1485.637 kb on + strand, within QF045_RS06595 at 1486.591 kb on + strand at 1486.651 kb on + strand at 1486.659 kb on - strand, within QF045_RS06605 at 1486.722 kb on + strand, within QF045_RS06605 at 1486.888 kb on + strand, within QF045_RS06605 at 1486.894 kb on + strand, within QF045_RS06605 at 1486.894 kb on + strand, within QF045_RS06605 at 1486.941 kb on + strand, within QF045_RS06605 at 1486.964 kb on - strand, within QF045_RS06605 at 1486.964 kb on - strand, within QF045_RS06605 at 1487.086 kb on - strand at 1487.089 kb on - strand at 1487.098 kb on + strand at 1487.098 kb on + strand at 1487.098 kb on + strand at 1487.098 kb on + strand at 1487.104 kb on - strand at 1487.106 kb on - strand at 1487.106 kb on - strand at 1487.106 kb on - strand at 1487.106 kb on - strand at 1487.106 kb on - strand at 1487.116 kb on + strand at 1487.144 kb on + strand at 1487.144 kb on + strand at 1487.145 kb on + strand at 1487.218 kb on + strand, within QF045_RS06610 at 1487.311 kb on - strand, within QF045_RS06610 at 1487.608 kb on + strand, within QF045_RS06615 at 1487.613 kb on + strand, within QF045_RS06615 at 1487.613 kb on + strand, within QF045_RS06615 at 1487.613 kb on + strand, within QF045_RS06615 at 1487.621 kb on - strand, within QF045_RS06615 at 1487.621 kb on - strand, within QF045_RS06615 at 1487.621 kb on - strand, within QF045_RS06615 at 1487.626 kb on - strand, within QF045_RS06615 at 1487.663 kb on - strand, within QF045_RS06615 at 1487.715 kb on + strand, within QF045_RS06615 at 1487.715 kb on + strand, within QF045_RS06615 at 1487.720 kb on + strand, within QF045_RS06615 at 1487.723 kb on - strand, within QF045_RS06615 at 1487.723 kb on - strand, within QF045_RS06615 at 1487.759 kb on - strand, within QF045_RS06615 at 1487.790 kb on + strand, within QF045_RS06615 at 1487.790 kb on + strand, within QF045_RS06615 at 1487.856 kb on - strand, within QF045_RS06615 at 1487.863 kb on + strand, within QF045_RS06615 at 1487.865 kb on + strand, within QF045_RS06615 at 1487.870 kb on + strand, within QF045_RS06615 at 1487.870 kb on + strand, within QF045_RS06615 at 1487.870 kb on + strand, within QF045_RS06615 at 1487.870 kb on + strand, within QF045_RS06615 at 1487.870 kb on + strand, within QF045_RS06615 at 1487.878 kb on - strand, within QF045_RS06615 at 1487.878 kb on - strand, within QF045_RS06615 at 1487.878 kb on - strand, within QF045_RS06615 at 1487.878 kb on - strand, within QF045_RS06615 at 1487.878 kb on - strand, within QF045_RS06615 at 1487.885 kb on + strand, within QF045_RS06615 at 1488.018 kb on + strand at 1488.018 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction no stress control with NaOH remove 1,485,637 + QF045_RS06595 0.90 -0.3 1,486,591 + -1.3 1,486,651 + -0.4 1,486,659 - QF045_RS06605 0.11 +0.0 1,486,722 + QF045_RS06605 0.23 -1.3 1,486,888 + QF045_RS06605 0.55 +0.2 1,486,894 + QF045_RS06605 0.56 -0.1 1,486,894 + QF045_RS06605 0.56 -1.1 1,486,941 + QF045_RS06605 0.66 +1.5 1,486,964 - QF045_RS06605 0.70 -2.2 1,486,964 - QF045_RS06605 0.70 -2.2 1,487,086 - +0.1 1,487,089 - -0.8 1,487,098 + -1.3 1,487,098 + -0.3 1,487,098 + -0.1 1,487,098 + +0.4 1,487,104 - -0.3 1,487,106 - -0.8 1,487,106 - -0.3 1,487,106 - +0.0 1,487,106 - +0.7 1,487,106 - +0.5 1,487,116 + +1.2 1,487,144 + -1.4 1,487,144 + +0.1 1,487,145 + -1.2 1,487,218 + QF045_RS06610 0.10 +0.2 1,487,311 - QF045_RS06610 0.41 -0.1 1,487,608 + QF045_RS06615 0.12 -0.4 1,487,613 + QF045_RS06615 0.13 +1.3 1,487,613 + QF045_RS06615 0.13 -1.1 1,487,613 + QF045_RS06615 0.13 +0.1 1,487,621 - QF045_RS06615 0.15 +0.4 1,487,621 - QF045_RS06615 0.15 -0.4 1,487,621 - QF045_RS06615 0.15 +0.4 1,487,626 - QF045_RS06615 0.16 +0.5 1,487,663 - QF045_RS06615 0.23 -0.4 1,487,715 + QF045_RS06615 0.33 +2.2 1,487,715 + QF045_RS06615 0.33 -0.1 1,487,720 + QF045_RS06615 0.34 -1.2 1,487,723 - QF045_RS06615 0.35 -0.2 1,487,723 - QF045_RS06615 0.35 +0.6 1,487,759 - QF045_RS06615 0.42 -1.7 1,487,790 + QF045_RS06615 0.48 +0.1 1,487,790 + QF045_RS06615 0.48 +0.3 1,487,856 - QF045_RS06615 0.61 +1.2 1,487,863 + QF045_RS06615 0.62 +0.0 1,487,865 + QF045_RS06615 0.63 -0.0 1,487,870 + QF045_RS06615 0.64 +0.2 1,487,870 + QF045_RS06615 0.64 +0.0 1,487,870 + QF045_RS06615 0.64 -0.6 1,487,870 + QF045_RS06615 0.64 -0.3 1,487,870 + QF045_RS06615 0.64 +1.1 1,487,878 - QF045_RS06615 0.65 +0.3 1,487,878 - QF045_RS06615 0.65 +0.3 1,487,878 - QF045_RS06615 0.65 +2.0 1,487,878 - QF045_RS06615 0.65 -0.9 1,487,878 - QF045_RS06615 0.65 -0.5 1,487,885 + QF045_RS06615 0.66 -0.2 1,488,018 + -0.2 1,488,018 + +0.6
Or see this region's nucleotide sequence