Strain Fitness in Pseudomonas orientalis W4I3 around QF045_RS06605

Experiment: no stress control with NaOH

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntQF045_RS06595 and QF045_RS06600 overlap by 8 nucleotidesQF045_RS06600 and QF045_RS06605 are separated by 99 nucleotidesQF045_RS06605 and QF045_RS06610 are separated by 66 nucleotidesQF045_RS06610 and QF045_RS06615 are separated by 54 nucleotidesQF045_RS06615 and QF045_RS06620 overlap by 8 nucleotides QF045_RS06595: QF045_RS06595 - molybdopterin-synthase adenylyltransferase MoeB, at 1,484,958 to 1,485,714 _RS06595 QF045_RS06600: QF045_RS06600 - glutamate racemase, at 1,485,707 to 1,486,501 _RS06600 QF045_RS06605: QF045_RS06605 - acyloxyacyl hydrolase, at 1,486,601 to 1,487,119 _RS06605 QF045_RS06610: QF045_RS06610 - YkgJ family cysteine cluster protein, at 1,487,186 to 1,487,491 _RS06610 QF045_RS06615: QF045_RS06615 - DUF2878 domain-containing protein, at 1,487,546 to 1,488,055 _RS06615 QF045_RS06620: QF045_RS06620 - cyclopropane-fatty-acyl-phospholipid synthase family protein, at 1,488,048 to 1,489,319 _RS06620 Position (kb) 1486 1487 1488Strain fitness (log2 ratio) -2 -1 0 1 2at 1485.637 kb on + strand, within QF045_RS06595at 1486.591 kb on + strandat 1486.651 kb on + strandat 1486.659 kb on - strand, within QF045_RS06605at 1486.722 kb on + strand, within QF045_RS06605at 1486.888 kb on + strand, within QF045_RS06605at 1486.894 kb on + strand, within QF045_RS06605at 1486.894 kb on + strand, within QF045_RS06605at 1486.941 kb on + strand, within QF045_RS06605at 1486.964 kb on - strand, within QF045_RS06605at 1486.964 kb on - strand, within QF045_RS06605at 1487.086 kb on - strandat 1487.089 kb on - strandat 1487.098 kb on + strandat 1487.098 kb on + strandat 1487.098 kb on + strandat 1487.098 kb on + strandat 1487.104 kb on - strandat 1487.106 kb on - strandat 1487.106 kb on - strandat 1487.106 kb on - strandat 1487.106 kb on - strandat 1487.106 kb on - strandat 1487.116 kb on + strandat 1487.144 kb on + strandat 1487.144 kb on + strandat 1487.145 kb on + strandat 1487.218 kb on + strand, within QF045_RS06610at 1487.311 kb on - strand, within QF045_RS06610at 1487.608 kb on + strand, within QF045_RS06615at 1487.613 kb on + strand, within QF045_RS06615at 1487.613 kb on + strand, within QF045_RS06615at 1487.613 kb on + strand, within QF045_RS06615at 1487.621 kb on - strand, within QF045_RS06615at 1487.621 kb on - strand, within QF045_RS06615at 1487.621 kb on - strand, within QF045_RS06615at 1487.626 kb on - strand, within QF045_RS06615at 1487.663 kb on - strand, within QF045_RS06615at 1487.715 kb on + strand, within QF045_RS06615at 1487.715 kb on + strand, within QF045_RS06615at 1487.720 kb on + strand, within QF045_RS06615at 1487.723 kb on - strand, within QF045_RS06615at 1487.723 kb on - strand, within QF045_RS06615at 1487.759 kb on - strand, within QF045_RS06615at 1487.790 kb on + strand, within QF045_RS06615at 1487.790 kb on + strand, within QF045_RS06615at 1487.856 kb on - strand, within QF045_RS06615at 1487.863 kb on + strand, within QF045_RS06615at 1487.865 kb on + strand, within QF045_RS06615at 1487.870 kb on + strand, within QF045_RS06615at 1487.870 kb on + strand, within QF045_RS06615at 1487.870 kb on + strand, within QF045_RS06615at 1487.870 kb on + strand, within QF045_RS06615at 1487.870 kb on + strand, within QF045_RS06615at 1487.878 kb on - strand, within QF045_RS06615at 1487.878 kb on - strand, within QF045_RS06615at 1487.878 kb on - strand, within QF045_RS06615at 1487.878 kb on - strand, within QF045_RS06615at 1487.878 kb on - strand, within QF045_RS06615at 1487.885 kb on + strand, within QF045_RS06615at 1488.018 kb on + strandat 1488.018 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction no stress control with NaOH
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1,485,637 + QF045_RS06595 0.90 -0.3
1,486,591 + -1.3
1,486,651 + -0.4
1,486,659 - QF045_RS06605 0.11 +0.0
1,486,722 + QF045_RS06605 0.23 -1.3
1,486,888 + QF045_RS06605 0.55 +0.2
1,486,894 + QF045_RS06605 0.56 -0.1
1,486,894 + QF045_RS06605 0.56 -1.1
1,486,941 + QF045_RS06605 0.66 +1.5
1,486,964 - QF045_RS06605 0.70 -2.2
1,486,964 - QF045_RS06605 0.70 -2.2
1,487,086 - +0.1
1,487,089 - -0.8
1,487,098 + -1.3
1,487,098 + -0.3
1,487,098 + -0.1
1,487,098 + +0.4
1,487,104 - -0.3
1,487,106 - -0.8
1,487,106 - -0.3
1,487,106 - +0.0
1,487,106 - +0.7
1,487,106 - +0.5
1,487,116 + +1.2
1,487,144 + -1.4
1,487,144 + +0.1
1,487,145 + -1.2
1,487,218 + QF045_RS06610 0.10 +0.2
1,487,311 - QF045_RS06610 0.41 -0.1
1,487,608 + QF045_RS06615 0.12 -0.4
1,487,613 + QF045_RS06615 0.13 +1.3
1,487,613 + QF045_RS06615 0.13 -1.1
1,487,613 + QF045_RS06615 0.13 +0.1
1,487,621 - QF045_RS06615 0.15 +0.4
1,487,621 - QF045_RS06615 0.15 -0.4
1,487,621 - QF045_RS06615 0.15 +0.4
1,487,626 - QF045_RS06615 0.16 +0.5
1,487,663 - QF045_RS06615 0.23 -0.4
1,487,715 + QF045_RS06615 0.33 +2.2
1,487,715 + QF045_RS06615 0.33 -0.1
1,487,720 + QF045_RS06615 0.34 -1.2
1,487,723 - QF045_RS06615 0.35 -0.2
1,487,723 - QF045_RS06615 0.35 +0.6
1,487,759 - QF045_RS06615 0.42 -1.7
1,487,790 + QF045_RS06615 0.48 +0.1
1,487,790 + QF045_RS06615 0.48 +0.3
1,487,856 - QF045_RS06615 0.61 +1.2
1,487,863 + QF045_RS06615 0.62 +0.0
1,487,865 + QF045_RS06615 0.63 -0.0
1,487,870 + QF045_RS06615 0.64 +0.2
1,487,870 + QF045_RS06615 0.64 +0.0
1,487,870 + QF045_RS06615 0.64 -0.6
1,487,870 + QF045_RS06615 0.64 -0.3
1,487,870 + QF045_RS06615 0.64 +1.1
1,487,878 - QF045_RS06615 0.65 +0.3
1,487,878 - QF045_RS06615 0.65 +0.3
1,487,878 - QF045_RS06615 0.65 +2.0
1,487,878 - QF045_RS06615 0.65 -0.9
1,487,878 - QF045_RS06615 0.65 -0.5
1,487,885 + QF045_RS06615 0.66 -0.2
1,488,018 + -0.2
1,488,018 + +0.6

Or see this region's nucleotide sequence