Experiment: Tween 20 (C), 20C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt A4249_RS10755 and A4249_RS10760 are separated by 114 nucleotides A4249_RS10760 and A4249_RS10765 overlap by 4 nucleotides A4249_RS10765 and A4249_RS10770 overlap by 2 nucleotides A4249_RS10770 and A4249_RS10775 are separated by 71 nucleotides A4249_RS10775 and A4249_RS10780 are separated by 14 nucleotides
A4249_RS10755: A4249_RS10755 - homogentisate 1,2-dioxygenase, at 2,161,232 to 2,162,527
_RS10755
A4249_RS10760: A4249_RS10760 - hypothetical protein, at 2,162,642 to 2,163,145
_RS10760
A4249_RS10765: A4249_RS10765 - Lrp/AsnC ligand binding domain-containing protein, at 2,163,142 to 2,163,399
_RS10765
A4249_RS10770: A4249_RS10770 - alpha/beta hydrolase, at 2,163,398 to 2,164,252
_RS10770
A4249_RS10775: A4249_RS10775 - bifunctional 4-hydroxy-2-oxoglutarate aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase, at 2,164,324 to 2,165,004
_RS10775
A4249_RS10780: A4249_RS10780 - periplasmic heavy metal sensor, at 2,165,019 to 2,165,531
_RS10780
Position (kb)
2163
2164
2165 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2162.407 kb on - strand at 2162.502 kb on + strand at 2162.502 kb on + strand at 2162.502 kb on + strand at 2162.502 kb on + strand at 2162.502 kb on + strand at 2162.503 kb on - strand at 2162.503 kb on - strand at 2162.503 kb on - strand at 2162.540 kb on + strand at 2162.541 kb on - strand at 2162.648 kb on + strand at 2162.648 kb on + strand at 2162.648 kb on + strand at 2162.648 kb on + strand at 2162.648 kb on + strand at 2162.648 kb on + strand at 2162.648 kb on + strand at 2162.648 kb on + strand at 2162.648 kb on + strand at 2162.648 kb on + strand at 2162.648 kb on + strand at 2162.648 kb on + strand at 2162.648 kb on + strand at 2162.648 kb on + strand at 2162.648 kb on + strand at 2162.648 kb on + strand at 2162.649 kb on - strand at 2162.649 kb on - strand at 2162.649 kb on - strand at 2162.649 kb on - strand at 2162.649 kb on - strand at 2162.649 kb on - strand at 2162.795 kb on + strand, within A4249_RS10760 at 2162.795 kb on + strand, within A4249_RS10760 at 2162.795 kb on + strand, within A4249_RS10760 at 2162.796 kb on - strand, within A4249_RS10760 at 2162.796 kb on - strand, within A4249_RS10760 at 2162.796 kb on - strand, within A4249_RS10760 at 2162.796 kb on - strand, within A4249_RS10760 at 2162.796 kb on - strand, within A4249_RS10760 at 2162.834 kb on + strand, within A4249_RS10760 at 2162.834 kb on + strand, within A4249_RS10760 at 2162.834 kb on + strand, within A4249_RS10760 at 2162.834 kb on + strand, within A4249_RS10760 at 2162.834 kb on + strand, within A4249_RS10760 at 2162.834 kb on + strand, within A4249_RS10760 at 2162.834 kb on + strand, within A4249_RS10760 at 2162.834 kb on + strand, within A4249_RS10760 at 2162.834 kb on + strand, within A4249_RS10760 at 2162.834 kb on + strand, within A4249_RS10760 at 2162.834 kb on + strand, within A4249_RS10760 at 2162.835 kb on - strand, within A4249_RS10760 at 2162.835 kb on - strand, within A4249_RS10760 at 2162.835 kb on - strand, within A4249_RS10760 at 2162.835 kb on - strand, within A4249_RS10760 at 2162.835 kb on - strand, within A4249_RS10760 at 2162.835 kb on - strand, within A4249_RS10760 at 2162.835 kb on - strand, within A4249_RS10760 at 2162.835 kb on - strand, within A4249_RS10760 at 2162.997 kb on - strand, within A4249_RS10760 at 2163.143 kb on + strand at 2163.144 kb on - strand at 2163.299 kb on + strand, within A4249_RS10765 at 2163.299 kb on + strand, within A4249_RS10765 at 2163.299 kb on + strand, within A4249_RS10765 at 2163.299 kb on + strand, within A4249_RS10765 at 2163.300 kb on - strand, within A4249_RS10765 at 2163.300 kb on - strand, within A4249_RS10765 at 2163.300 kb on - strand, within A4249_RS10765 at 2163.300 kb on - strand, within A4249_RS10765 at 2163.300 kb on - strand, within A4249_RS10765 at 2163.300 kb on - strand, within A4249_RS10765 at 2163.300 kb on - strand, within A4249_RS10765 at 2163.300 kb on - strand, within A4249_RS10765 at 2163.356 kb on + strand, within A4249_RS10765 at 2163.432 kb on + strand at 2163.433 kb on - strand at 2163.491 kb on + strand, within A4249_RS10770 at 2163.491 kb on + strand, within A4249_RS10770 at 2163.491 kb on + strand, within A4249_RS10770 at 2163.492 kb on - strand, within A4249_RS10770 at 2163.492 kb on - strand, within A4249_RS10770 at 2163.492 kb on - strand, within A4249_RS10770 at 2163.492 kb on - strand, within A4249_RS10770 at 2163.492 kb on - strand, within A4249_RS10770 at 2163.492 kb on - strand, within A4249_RS10770 at 2163.492 kb on - strand, within A4249_RS10770 at 2163.593 kb on + strand, within A4249_RS10770 at 2163.593 kb on + strand, within A4249_RS10770 at 2163.593 kb on + strand, within A4249_RS10770 at 2163.758 kb on + strand, within A4249_RS10770 at 2163.758 kb on + strand, within A4249_RS10770 at 2163.758 kb on + strand, within A4249_RS10770 at 2163.758 kb on + strand, within A4249_RS10770 at 2163.758 kb on + strand, within A4249_RS10770 at 2163.759 kb on - strand, within A4249_RS10770 at 2163.759 kb on - strand, within A4249_RS10770 at 2163.759 kb on - strand, within A4249_RS10770 at 2163.759 kb on - strand, within A4249_RS10770 at 2163.759 kb on - strand, within A4249_RS10770 at 2163.759 kb on - strand, within A4249_RS10770 at 2163.759 kb on - strand, within A4249_RS10770 at 2163.759 kb on - strand, within A4249_RS10770 at 2163.881 kb on - strand, within A4249_RS10770 at 2163.881 kb on - strand, within A4249_RS10770 at 2164.304 kb on + strand at 2164.304 kb on + strand at 2164.304 kb on + strand at 2164.304 kb on + strand at 2164.304 kb on + strand at 2164.304 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Tween 20 (C), 20C remove 2,162,407 - -1.0 2,162,502 + +0.7 2,162,502 + +0.0 2,162,502 + -1.3 2,162,502 + +0.1 2,162,502 + -1.2 2,162,503 - -1.0 2,162,503 - +1.0 2,162,503 - +1.0 2,162,540 + -0.3 2,162,541 - +0.0 2,162,648 + -1.6 2,162,648 + -1.3 2,162,648 + +1.6 2,162,648 + -0.4 2,162,648 + +0.2 2,162,648 + +2.5 2,162,648 + -0.1 2,162,648 + -1.2 2,162,648 + +0.3 2,162,648 + -1.1 2,162,648 + +0.0 2,162,648 + -0.6 2,162,648 + -1.0 2,162,648 + -0.6 2,162,648 + +0.6 2,162,648 + +0.6 2,162,649 - +0.2 2,162,649 - +0.0 2,162,649 - +0.5 2,162,649 - -0.4 2,162,649 - +0.3 2,162,649 - -0.8 2,162,795 + A4249_RS10760 0.30 +0.3 2,162,795 + A4249_RS10760 0.30 +0.3 2,162,795 + A4249_RS10760 0.30 +1.2 2,162,796 - A4249_RS10760 0.31 +0.4 2,162,796 - A4249_RS10760 0.31 -0.8 2,162,796 - A4249_RS10760 0.31 -1.6 2,162,796 - A4249_RS10760 0.31 +1.8 2,162,796 - A4249_RS10760 0.31 +0.0 2,162,834 + A4249_RS10760 0.38 +0.1 2,162,834 + A4249_RS10760 0.38 -0.6 2,162,834 + A4249_RS10760 0.38 -1.2 2,162,834 + A4249_RS10760 0.38 +0.3 2,162,834 + A4249_RS10760 0.38 +1.6 2,162,834 + A4249_RS10760 0.38 +0.4 2,162,834 + A4249_RS10760 0.38 -1.5 2,162,834 + A4249_RS10760 0.38 +0.2 2,162,834 + A4249_RS10760 0.38 -0.3 2,162,834 + A4249_RS10760 0.38 -0.1 2,162,834 + A4249_RS10760 0.38 +0.2 2,162,835 - A4249_RS10760 0.38 -2.8 2,162,835 - A4249_RS10760 0.38 +0.3 2,162,835 - A4249_RS10760 0.38 -1.0 2,162,835 - A4249_RS10760 0.38 -0.4 2,162,835 - A4249_RS10760 0.38 +0.0 2,162,835 - A4249_RS10760 0.38 -0.4 2,162,835 - A4249_RS10760 0.38 +1.6 2,162,835 - A4249_RS10760 0.38 +0.4 2,162,997 - A4249_RS10760 0.70 +2.0 2,163,143 + -0.7 2,163,144 - -1.2 2,163,299 + A4249_RS10765 0.61 -0.8 2,163,299 + A4249_RS10765 0.61 -0.8 2,163,299 + A4249_RS10765 0.61 +0.4 2,163,299 + A4249_RS10765 0.61 -0.2 2,163,300 - A4249_RS10765 0.61 -1.4 2,163,300 - A4249_RS10765 0.61 +1.1 2,163,300 - A4249_RS10765 0.61 +0.0 2,163,300 - A4249_RS10765 0.61 +0.0 2,163,300 - A4249_RS10765 0.61 -0.9 2,163,300 - A4249_RS10765 0.61 +0.0 2,163,300 - A4249_RS10765 0.61 -1.6 2,163,300 - A4249_RS10765 0.61 -0.6 2,163,356 + A4249_RS10765 0.83 -0.1 2,163,432 + +0.8 2,163,433 - +0.5 2,163,491 + A4249_RS10770 0.11 +0.4 2,163,491 + A4249_RS10770 0.11 +0.1 2,163,491 + A4249_RS10770 0.11 +0.0 2,163,492 - A4249_RS10770 0.11 +0.4 2,163,492 - A4249_RS10770 0.11 -3.2 2,163,492 - A4249_RS10770 0.11 +0.6 2,163,492 - A4249_RS10770 0.11 +0.2 2,163,492 - A4249_RS10770 0.11 +1.2 2,163,492 - A4249_RS10770 0.11 -0.6 2,163,492 - A4249_RS10770 0.11 +0.7 2,163,593 + A4249_RS10770 0.23 -0.9 2,163,593 + A4249_RS10770 0.23 +0.4 2,163,593 + A4249_RS10770 0.23 +0.9 2,163,758 + A4249_RS10770 0.42 +0.0 2,163,758 + A4249_RS10770 0.42 -1.3 2,163,758 + A4249_RS10770 0.42 +0.8 2,163,758 + A4249_RS10770 0.42 +0.4 2,163,758 + A4249_RS10770 0.42 -0.2 2,163,759 - A4249_RS10770 0.42 -0.4 2,163,759 - A4249_RS10770 0.42 +1.1 2,163,759 - A4249_RS10770 0.42 +0.4 2,163,759 - A4249_RS10770 0.42 -1.7 2,163,759 - A4249_RS10770 0.42 +2.0 2,163,759 - A4249_RS10770 0.42 -0.2 2,163,759 - A4249_RS10770 0.42 +0.3 2,163,759 - A4249_RS10770 0.42 +0.0 2,163,881 - A4249_RS10770 0.56 +0.3 2,163,881 - A4249_RS10770 0.56 -1.2 2,164,304 + +0.6 2,164,304 + -0.4 2,164,304 + -0.4 2,164,304 + -0.1 2,164,304 + -0.3 2,164,304 + -0.3
Or see this region's nucleotide sequence